TS1.15H

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$45.00 to $425.00
SKU: TS1.15H
View product citations for antibody TS1.15H on CiteAb

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Available: 158

DSHB Data Sheet

Catalog Fields

Antigen: Jagged 1 protein
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Artavanis-Tsakonas, S.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Harvard Medical School
Antigen Molecular Weight: 134 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Intracellular domain-GST fusion protein
Gene: JAG1
Alternate Antibody Name:
Alternate Gene Names: AGS, AHD, AWS, CD339, HJ1, JAGL1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Ag8 cells
Epitope Mapped: No
Uniprot ID: P78504 
Epitope Location or Sequence: Intracellular domain of human jagged1
Entrez Gene ID: 182 
Immunogen Sequence: Protein domain
Antibody Registry ID: AB_528317 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
Additional Information: RRID:AB_528317
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1.15H was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product TS1.15H)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Human ligands of the Notch receptor.
    Artavanis-Tsakonas S
    The American journal of pathology 154.3 (1999 Mar): 785-94.

    IF References

    Human ligands of the Notch receptor.
    Artavanis-Tsakonas S
    The American journal of pathology 154.3 (1999 Mar): 785-94.

    Six2 and Wnt regulate self-renewal and commitment of nephron progenitors through shared gene regulatory networks.
    McMahon AP
    Developmental cell 23.3 (2012 Sep 11): 637-51.

    Single cell dissection of early kidney development: multilineage priming.
    Potter SS
    Development (Cambridge, England) 141.15 (2014 Aug): 3093-101.

    Molecular signatures identify immature mesenchymal progenitors in early mouse limb buds that respond differentially to morphogen signaling.
    Zeller R
    Development (Cambridge, England) 146.10 (2019 May 28): .

    WB References

    Human ligands of the Notch receptor.
    Artavanis-Tsakonas S
    The American journal of pathology 154.3 (1999 Mar): 785-94.

    Constitutively activated Notch signaling is involved in survival and apoptosis resistance of B-CLL cells.
    Marconi P
    Blood 113.4 (2009 Jan 22): 856-65.

    Notch-independent regulation of Hes-1 expression by c-Jun N-terminal kinase signaling in human endothelial cells.
    Foreman KE
    Laboratory investigation; a journal of technical methods and pathology 86.8 (2006 Aug): 842-52.

    Meningioma transcript profiles reveal deregulated Notch signaling pathway.
    Lal A
    Cancer research 65.12 (2005 Jun 15): 5070-5.

    Notch1 modulates mesenchymal stem cells mediated regulatory T-cell induction.
    Di Ianni M
    European journal of immunology 43.1 (2013 Jan): 182-7.

    The Hajdu Cheney mutation sensitizes mice to the osteolytic actions of tumor necrosis factor α.
    Canalis E
    The Journal of biological chemistry 294.39 (2019 Sep 27): 14203-14214.

    IHC References
    FFPE References

    Human ligands of the Notch receptor.
    Artavanis-Tsakonas S
    The American journal of pathology 154.3 (1999 Mar): 785-94.

    All References

    Human ligands of the Notch receptor.
    Artavanis-Tsakonas S
    The American journal of pathology 154.3 (1999 Mar): 785-94.

    Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma.
    Dörken B
    Blood 99.9 (2002 May 1): 3398-403.

    An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis.
    Sham MH
    eLife 6. (2017 Nov 15): .

    Six2 and Wnt regulate self-renewal and commitment of nephron progenitors through shared gene regulatory networks.
    McMahon AP
    Developmental cell 23.3 (2012 Sep 11): 637-51.

    Single cell dissection of early kidney development: multilineage priming.
    Potter SS
    Development (Cambridge, England) 141.15 (2014 Aug): 3093-101.

    Molecular signatures identify immature mesenchymal progenitors in early mouse limb buds that respond differentially to morphogen signaling.
    Zeller R
    Development (Cambridge, England) 146.10 (2019 May 28): .

    Constitutively activated Notch signaling is involved in survival and apoptosis resistance of B-CLL cells.
    Marconi P
    Blood 113.4 (2009 Jan 22): 856-65.

    Notch-independent regulation of Hes-1 expression by c-Jun N-terminal kinase signaling in human endothelial cells.
    Foreman KE
    Laboratory investigation; a journal of technical methods and pathology 86.8 (2006 Aug): 842-52.

    Meningioma transcript profiles reveal deregulated Notch signaling pathway.
    Lal A
    Cancer research 65.12 (2005 Jun 15): 5070-5.

    Notch1 modulates mesenchymal stem cells mediated regulatory T-cell induction.
    Di Ianni M
    European journal of immunology 43.1 (2013 Jan): 182-7.

    The Hajdu Cheney mutation sensitizes mice to the osteolytic actions of tumor necrosis factor α.
    Canalis E
    The Journal of biological chemistry 294.39 (2019 Sep 27): 14203-14214.

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