TS1.15H
Catalog Fields
Clone ID/Product Name: TS1.15H
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: JAG1
Ab Isotype: RIgG2a
Gene Name:
Entrez Gene ID: 182
Clonality: Monoclonal
Immunogen: Intracellular domain-GST fusion protein
Clone:
Immunogen Sequence: Protein domain
Myeloma Strain: Ag8 cells
Epitope Mapped: No
Antigen Name: Jagged 1 protein
Epitope Location or Sequence: Intracellular domain of human jagged1
Alternate Antigen Name:
Deposit Date: 5/2/2000
Antigen Molecular Weight: 134 kDa
Depositor: Artavanis-Tsakonas, S.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Human
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_528317
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1.15H was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product TS1.15H)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Human ligands of the Notch receptor.
Artavanis-Tsakonas S
The American journal of pathology 154.3 (1999 Mar): 785-94.