Cmnb-1

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$40.00
SKU: Cmnb-1
View product citations for antibody Cmnb-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Numb
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Saner, C.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: ISREC, Epalinges, Switzerland
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Recognizes all isoforms by western blotting.
Antigen Molecular Weight: Predicted (multiple isoforms): 64, 66, 69, and 71 kDa; Apparent: 65, 66, 71, and 72 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Numb
Immunogen: Recombinant GST/Numb (C-terminal 491 aa from long isoform) fusion protein
Alternate Gene Names: Nb
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: x63Ag8.653
Epitope Location or Sequence: aa 163-653 (long isoform)
Uniprot ID: Q9QZS3 
Immunogen Sequence: Partial protein
Entrez Gene ID: 18222 
Additional Characterization:  
Antibody Registry ID: AB_528417 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cmnb-1 was deposited to the DSHB by Saner, C. (DSHB Hybridoma Product Cmnb-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Multiple roles of mouse Numb in tuning developmental cell fates.
    Aguet M
    Current biology : CB 11.7 (2001 Apr 3): 494-501.

    IF References

    Portrayal of the Notch system in embryonic stem cell-derived embryoid bodies.
    Cidadão A
    Cells, tissues, organs 193.4 (2011): 239-52.

    WB References

    Multiple roles of mouse Numb in tuning developmental cell fates.
    Aguet M
    Current biology : CB 11.7 (2001 Apr 3): 494-501.

    All References

    Portrayal of the Notch system in embryonic stem cell-derived embryoid bodies.
    Cidadão A
    Cells, tissues, organs 193.4 (2011): 239-52.

    Multiple roles of mouse Numb in tuning developmental cell fates.
    Aguet M
    Current biology : CB 11.7 (2001 Apr 3): 494-501.

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