Cmnb-1

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SKU: Cmnb-1

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: Cmnb-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Numb
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_528417 
Uniprot ID: Q9QZS3 
RRID:  
Entrez Gene ID: 18222 
Clonality: Monoclonal
Immunogen: Recombinant GST/Numb (C-terminal 491 aa from long isoform) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: x63Ag8.653
Epitope Mapped: Yes
Antigen Name: Numb
Epitope Location or Sequence: aa 163-653 (long isoform)
Alternate Antigen Name:
Deposit Date: 12/21/2004
Antigen Molecular Weight: Predicted (multiple isoforms): 64, 66, 69, and 71 kDa; Apparent: 65, 66, 71, and 72 kDa
Depositor: Saner, C.
Antigen Sequence:
Depositor Institution: ISREC, Epalinges, Switzerland
Antigen Species: mouse
Depositor Notes: Recognizes all isoforms by western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cmnb-1 was deposited to the DSHB by Saner, C. (DSHB Hybridoma Product Cmnb-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Multiple roles of mouse Numb in tuning developmental cell fates.
    Aguet M
    Current biology : CB 11.7 (2001 Apr 3): 494-501.

    IF References

    Portrayal of the Notch system in embryonic stem cell-derived embryoid bodies.
    Cidadão A
    Cells, tissues, organs 193.4 (2011): 239-52.

    WB References

    Multiple roles of mouse Numb in tuning developmental cell fates.
    Aguet M
    Current biology : CB 11.7 (2001 Apr 3): 494-501.

    All References

    Portrayal of the Notch system in embryonic stem cell-derived embryoid bodies.
    Cidadão A
    Cells, tissues, organs 193.4 (2011): 239-52.

    Multiple roles of mouse Numb in tuning developmental cell fates.
    Aguet M
    Current biology : CB 11.7 (2001 Apr 3): 494-501.

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