C651.6DbHN
Catalog Fields
Antigen: Notch2 intracellular domain (human)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Artavanis-Tsakonas, S.
Isotype: RIgG1, kappa light chain
Depositors Institution: Harvard Medical School
Host Species: rat
Depositors Notes: Works well for Immunoprecipitation
Positive Tested Species Reactivity: Canine, Human, Mouse
Gene: NOTCH2
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110 kDa
Alternate Gene Names: hN2; AGS2; HJCYS
Immunogen: Recombinant fusion protein GST-Notch2 intrcellular domain
Clonality: Monoclonal
Alternate Antibody Name: 6D, bhN6D
Epitope Mapped: Yes
Myeloma Strain: Ag8/ATCC CRL1580
Epitope Location or Sequence: intracellular domain
Uniprot ID: Q04721
Immunogen Sequence: Partial protein
Entrez Gene ID: 4853
Antibody Registry ID: AB_528412
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C651.6DbHN was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C651.6DbHN)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Alterations in Notch signaling in neoplastic lesions of the human cervix.
Artavanis-Tsakonas S
Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.