C651.6DbHN

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$40.00
SKU: C651.6DbHN
View product citations for antibody C651.6DbHN on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Notch2 intracellular domain (human)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Artavanis-Tsakonas, S.
Isotype: RIgG1, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Notes: Works well for Immunoprecipitation
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: NOTCH2
Immunogen: Recombinant fusion protein GST-Notch2 intrcellular domain
Alternate Gene Names: hN2; AGS2; HJCYS
Alternate Antibody Name: 6D, bhN6D
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8/ATCC CRL1580
Epitope Location or Sequence: intracellular domain
Uniprot ID: Q04721 
Immunogen Sequence: Partial protein
Entrez Gene ID: 4853 
Additional Characterization:  
Antibody Registry ID: AB_528412 
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C651.6DbHN was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C651.6DbHN)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    IF References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    TCF4 deficiency expands ventral diencephalon signaling and increases induction of pituitary progenitors.
    Camper SA
    Developmental biology 311.2 (2007 Nov 15): 396-407.

    Mechanisms underlying pituitary hypoplasia and failed cell specification in Lhx3-deficient mice.
    Camper SA
    Developmental biology 313.1 (2008 Jan 1): 118-29.

    Developmental regulation of Notch signaling genes in the embryonic pituitary: Prop1 deficiency affects Notch2 expression.
    Thomas PQ
    Developmental biology 265.2 (2004 Jan 15): 329-40.

    Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2.
    Zhao H
    Calcified tissue international 96.5 (2015 May): 465-75.

    WB References
    IHC References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Opposing role of Notch1 and Notch2 in a Kras(G12D)-driven murine non-small cell lung cancer model.
    Dechow T
    Oncogene 34.5 (2015 Jan 29): 578-88.

    Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.
    Xu K
    Oncogene 35.19 (2016 May 12): 2485-95.

    Expression and clinicopathological significance of notch signaling and cell-fate genes in biliary tract cancer.
    Siveke JT
    The American journal of gastroenterology 107.1 (2012 Jan): 126-35.

    Notch signaling controls hepatoblast differentiation by altering the expression of liver-enriched transcription factors.
    Miyajima A
    Journal of cell science 117.Pt 15 (2004 Jul 1): 3165-74.

    FACS References

    The Notch2-Jagged1 interaction mediates stem cell factor signaling in erythropoiesis.
    De Maria R
    Cell death and differentiation 18.2 (2011 Feb): 371-80.

    Epitope Map References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    All References

    Alterations in Notch signaling in neoplastic lesions of the human cervix.
    Artavanis-Tsakonas S
    Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.

    Opposing role of Notch1 and Notch2 in a Kras(G12D)-driven murine non-small cell lung cancer model.
    Dechow T
    Oncogene 34.5 (2015 Jan 29): 578-88.

    Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.
    Xu K
    Oncogene 35.19 (2016 May 12): 2485-95.

    Expression and clinicopathological significance of notch signaling and cell-fate genes in biliary tract cancer.
    Siveke JT
    The American journal of gastroenterology 107.1 (2012 Jan): 126-35.

    Notch signaling controls hepatoblast differentiation by altering the expression of liver-enriched transcription factors.
    Miyajima A
    Journal of cell science 117.Pt 15 (2004 Jul 1): 3165-74.

    TCF4 deficiency expands ventral diencephalon signaling and increases induction of pituitary progenitors.
    Camper SA
    Developmental biology 311.2 (2007 Nov 15): 396-407.

    Mechanisms underlying pituitary hypoplasia and failed cell specification in Lhx3-deficient mice.
    Camper SA
    Developmental biology 313.1 (2008 Jan 1): 118-29.

    Developmental regulation of Notch signaling genes in the embryonic pituitary: Prop1 deficiency affects Notch2 expression.
    Thomas PQ
    Developmental biology 265.2 (2004 Jan 15): 329-40.

    Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2.
    Zhao H
    Calcified tissue international 96.5 (2015 May): 465-75.

    Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane.
    Artavanis-Tsakonas S
    Cell 90.2 (1997 Jul 25): 281-91.

    17β-estradiol enhances signalling mediated by VEGF-A-delta-like ligand 4-notch1 axis in human endothelial cells.
    Ferrari R
    PloS one 8.8 (2013): e71440.

    Involvement of Notch-1 signaling in bone marrow stroma-mediated de novo drug resistance of myeloma and other malignant lymphoid cell lines.
    Gabrilovich DI
    Blood 103.9 (2004 May 1): 3503-10.

    Notch signaling is inactive but inducible in human embryonic stem cells.
    Condie BG
    Stem cells (Dayton, Ohio) 24.7 (2006 Jul): 1646-53.

    The Notch2-Jagged1 interaction mediates stem cell factor signaling in erythropoiesis.
    De Maria R
    Cell death and differentiation 18.2 (2011 Feb): 371-80.

    The Hajdu Cheney mutation sensitizes mice to the osteolytic actions of tumor necrosis factor α.
    Canalis E
    The Journal of biological chemistry 294.39 (2019 Sep 27): 14203-14214.

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