C651.6DbHN
Catalog Fields
Antigen: Notch2 intracellular domain (human)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Artavanis-Tsakonas, S.
Isotype: RIgG1, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Notes: Works well for Immunoprecipitation. For IHC, citrate buffer antigen retrieval is recommended.
Antigen Molecular Weight: Apparent: 300 kDa processed to give a major intracellular product of 110 kDa
Predicted Species Reactivity:
Gene: NOTCH2
Immunogen: Recombinant fusion protein GST-Notch2 intrcellular domain
Alternate Gene Names: hN2; AGS2; HJCYS
Alternate Antibody Name: 6D, bhN6D
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8/ATCC CRL1580
Epitope Location or Sequence: intracellular domain
Uniprot ID: Q04721
Immunogen Sequence: Partial protein
Entrez Gene ID: 4853
Antibody Registry ID: AB_528412
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C651.6DbHN was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C651.6DbHN)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
16 References
Initial Publication
Alterations in Notch signaling in neoplastic lesions of the human cervix.
Artavanis-Tsakonas S
Proceedings of the National Academy of Sciences of the United States of America 92.14 (1995 Jul 3): 6414-8.