aTH
Catalog Fields
Antigen: Tyrosine hydroxylase (Tyrosine 3-monooxygenase)
Available to For-Profits: Yes
Antigen Species: Quail
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Le Douarin, N. / Ziller, C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Cuttlefish, Quail
Depositors Institution: Institut d’Embryologie Cellulaire et Moleculaire du C.N.R.S. et du College de France
Antigen Molecular Weight: Predicted: 56 kDa; Apparent: 63 kDa
Depositors Notes: This antibody gives an intracytoplasmic staining of dopaminergic neurons.
Immunogen: Recombinant TH (aa 60-368)/beta galactosidase fusion protein
Gene: TH
Alternate Antibody Name: alphaQTOH
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P11982
Epitope Location or Sequence: aa 60-368
Entrez Gene ID: 107314555
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528490
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: This protein is involved in the synthesis of dopamine from L-tyrosine.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
aTH was deposited to the DSHB by Le Douarin, N. / Ziller, C. (DSHB Hybridoma Product aTH)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
A monoclonal antibody directed against quail tyrosine hydroxylase: description and use in immunocytochemical studies on differentiating neural crest cells.
Ziller C
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 37.8 (1989 Aug): 1197-205.