aTH

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$40.00
SKU: aTH
View product citations for antibody aTH on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Tyrosine hydroxylase (Tyrosine 3-monooxygenase)
Hybridoma Cells Available: Yes
Antigen Species: Quail
Depositor: Le Douarin, N. / Ziller, C.
Isotype: MIgG2a
Depositors Institution: Institut d’Embryologie Cellulaire et Moleculaire du C.N.R.S. et du College de France, Nogent sur Marne
Host Species: mouse
Depositors Notes: This antibody gives an intracytoplasmic staining of dopaminergic neurons.
Positive Tested Species Reactivity: Chicken, Cuttlefish, Quail
Gene: TH
Antigen Molecular Weight: Predicted: 56 kDa; Apparent: 63 kDa
Clonality: Monoclonal
Immunogen: Recombinant TH (aa 60-368)/beta galactosidase fusion protein
Epitope Mapped: Yes
Alternate Antibody Name: alphaQTOH
Epitope Location or Sequence: aa 60-368
Myeloma Strain: SP2/0
Immunogen Sequence: Partial protein
Uniprot ID: P11982 
Additional Information: This protein is involved in the synthesis of dopamine from L-tyrosine.
Entrez Gene ID: 107314555 
Antibody Registry ID: AB_528490 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
aTH was deposited to the DSHB by Le Douarin, N. / Ziller, C. (DSHB Hybridoma Product aTH)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody directed against quail tyrosine hydroxylase: description and use in immunocytochemical studies on differentiating neural crest cells.
    Ziller C
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 37.8 (1989 Aug): 1197-205.

    IF References

    A monoclonal antibody directed against quail tyrosine hydroxylase: description and use in immunocytochemical studies on differentiating neural crest cells.
    Ziller C
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 37.8 (1989 Aug): 1197-205.

    Restricted response of mesencephalic neural crest to sympathetic differentiation signals in the trunk.
    Baker CV
    Developmental biology 278.1 (2005 Feb 1): 175-92.

    CtBP2 downregulation during neural crest specification induces expression of Mitf and REST, resulting in melanocyte differentiation and sympathoadrenal lineage suppression.
    Andrisani OM
    Molecular and cellular biology 31.5 (2011 Mar): 955-70.

    Origins of the avian neural crest: the role of neural plate-epidermal interactions.
    Bronner-Fraser M
    Development (Cambridge, England) 121.2 (1995 Feb): 525-38.

    The tyrosine hydroxylase gene is expressed in endoderm and pancreas of early quail embryos.
    Fauquet M
    Anatomy and embryology 189.4 (1994 Apr): 307-15.

    Effect of insulin and insulin-like growth factor I on the expression of the catecholaminergic phenotype by neural crest cells.
    Monier S
    Brain research. Developmental brain research 69.1 (1992 Sep 18): 59-66.

    The expression of neuropeptide Y immunoreactivity in the avian sympathoadrenal system conforms with two models of coexpression development for neurons and chromaffin cells.
    Chévere-Colón I
    Development (Cambridge, England) 115.2 (1992 Jun): 617-27.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    WB References
    IHC References

    A monoclonal antibody directed against quail tyrosine hydroxylase: description and use in immunocytochemical studies on differentiating neural crest cells.
    Ziller C
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 37.8 (1989 Aug): 1197-205.

    Evidence of early nervous differentiation and early catecholaminergic sensory system during Sepia officinalis embryogenesis.
    Bonnaud L
    The Journal of comparative neurology 517.4 (2009 Dec 1): 539-49.

    Epitope Map References

    A monoclonal antibody directed against quail tyrosine hydroxylase: description and use in immunocytochemical studies on differentiating neural crest cells.
    Ziller C
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 37.8 (1989 Aug): 1197-205.

    All References

    A monoclonal antibody directed against quail tyrosine hydroxylase: description and use in immunocytochemical studies on differentiating neural crest cells.
    Ziller C
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 37.8 (1989 Aug): 1197-205.

    Evidence of early nervous differentiation and early catecholaminergic sensory system during Sepia officinalis embryogenesis.
    Bonnaud L
    The Journal of comparative neurology 517.4 (2009 Dec 1): 539-49.

    Restricted response of mesencephalic neural crest to sympathetic differentiation signals in the trunk.
    Baker CV
    Developmental biology 278.1 (2005 Feb 1): 175-92.

    CtBP2 downregulation during neural crest specification induces expression of Mitf and REST, resulting in melanocyte differentiation and sympathoadrenal lineage suppression.
    Andrisani OM
    Molecular and cellular biology 31.5 (2011 Mar): 955-70.

    Origins of the avian neural crest: the role of neural plate-epidermal interactions.
    Bronner-Fraser M
    Development (Cambridge, England) 121.2 (1995 Feb): 525-38.

    The tyrosine hydroxylase gene is expressed in endoderm and pancreas of early quail embryos.
    Fauquet M
    Anatomy and embryology 189.4 (1994 Apr): 307-15.

    Effect of insulin and insulin-like growth factor I on the expression of the catecholaminergic phenotype by neural crest cells.
    Monier S
    Brain research. Developmental brain research 69.1 (1992 Sep 18): 59-66.

    The expression of neuropeptide Y immunoreactivity in the avian sympathoadrenal system conforms with two models of coexpression development for neurons and chromaffin cells.
    Chévere-Colón I
    Development (Cambridge, England) 115.2 (1992 Jun): 617-27.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Novel expression patterns of Pax3/Pax7 in early trunk neural crest and its melanocyte and non-melanocyte lineages in amniote embryos.
    Domínguez L
    Pigment cell research 18.4 (2005 Aug): 243-51.

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