Ubx/ABD-A FP6.87
Catalog Fields
Antigen: Ultrabithorax and abdominal-A proteins (all isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Crustacean, Drosophila, Drosophila virilis, Gerridae, Grasshopper, Limnoporus, Manduca, Metrobates, Onychophora, Rhagovelia, Spider
Depositors Notes: Broad species specificity
Predicted Species Reactivity:
Gene: Ubx/abd-A
Immunogen: Recombinant Drosophila Ultrabithorax lacking the first 26 aa from N-terminus fusion protein
Alternate Gene Names: CG10388, bx, Cbx, pbx, abx, bxd, DmUbx
Alternate Antibody Name: FP6.87, anti-UbdA
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 350-363 (Uniprot# P83949-1) region contains at least part of the epitope.
Uniprot ID: P83949 P29555
Immunogen Sequence: Partial protein
Entrez Gene ID: 42034 42037
Antibody Registry ID: AB_10660834
Additional Information: This antibody cross-reacts with abdominal-A and recognizes all isoforms of the two proteins. Based on sequence homology, this antibody should also work in Aedes, Bombyx, Apis, and Tribolium [PMID 7514518].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ubx/ABD-A FP6.87 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Ubx/ABD-A FP6.87)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins.
Akam M
Developmental genetics 15.1 (1994): 19-31.