Antigen: Ultrabithorax and abdominal-A proteins (all isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Crustacean, Drosophila, Drosophila virilis, Gerridae, Grasshopper, Limnoporus, Manduca, Metrobates, Onychophora, Rhagovelia, Spider
Depositors Notes: Broad species specificity
Antigen Molecular Weight: Predicted: multiple isoforms, from 35 to 40kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant Drosophila Ultrabithorax lacking the first 26 aa from N-terminus fusion protein
Alternate Gene Names: CG10388, bx, Cbx, pbx, abx, bxd, DmUbx
Alternate Antibody Name: FP6.87, anti-UbdA
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 350-363 (Uniprot# P83949-1) region contains at least part of the epitope.
Uniprot ID: P83949 P29555
Immunogen Sequence: Partial protein
Entrez Gene ID: 42034 42037
Antibody Registry ID: AB_10660834
Additional Information: This antibody cross-reacts with abdominal-A and recognizes all isoforms of the two proteins. Based on sequence homology, this antibody should also work in Aedes, Bombyx, Apis, and Tribolium [PMID 7514518].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ubx/ABD-A FP6.87 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Ubx/ABD-A FP6.87)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References