Ubx/ABD-A FP6.87
Catalog Fields
Clone ID/Product Name: Ubx/ABD-A FP6.87
Available to For-Profits: Yes
Alternate Antibody Name: FP6.87, anti-UbdA
Gene Symbol: Ubx/abd-A
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_10660834
Uniprot ID: P83949 P29555
Entrez Gene ID: 42034 42037
Clonality: Monoclonal
Immunogen: Recombinant Drosophila Ultrabithorax lacking the first 26 aa from N-terminus fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NSO
Epitope Mapped: Yes
Antigen Name: Ultrabithorax and abdominal-A proteins (all isoforms)
Epitope Location or Sequence: aa 350-363 (Uniprot# P83949-1) region contains at least part of the epitope.
Alternate Antigen Name:
Deposit Date: 5/25/2010
Antigen Molecular Weight: Predicted: multiple isoforms, from 35 to 40kDa
Depositor: White, R.
Antigen Sequence:
Depositor Institution: University of Cambridge
Antigen Species: Drosophila
Depositor Notes: Broad species specificity
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Crustacean, Drosophila, Drosophila virilis, Gerridae, Grasshopper, Limnoporus, Manduca, Metrobates, Onychophora, Rhagovelia, Spider
Additional Information: This antibody cross-reacts with abdominal-A and recognizes all isoforms of the two proteins. Based on sequence homology, this antibody should also work in Aedes, Bombyx, Apis, and Tribolium [PMID 7514518].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ubx/ABD-A FP6.87 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Ubx/ABD-A FP6.87)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins.
Akam M
Developmental genetics 15.1 (1994): 19-31.