Ubx/ABD-A FP6.87

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$40.00
SKU: Ubx/ABD-A FP6.87
View product citations for antibody Ubx/ABD-A FP6.87 on CiteAb

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Available: 112

DSHB Data Sheet

Catalog Fields

Antigen: Ultrabithorax and abdominal-A proteins (all isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Crustacean, Drosophila, Drosophila virilis, Gerridae, Grasshopper, Limnoporus, Manduca, Metrobates, Onychophora, Rhagovelia, Spider
Depositors Notes: Broad species specificity
Antigen Molecular Weight: Predicted: multiple isoforms, from 35 to 40kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ubx/abd-A
Immunogen: Recombinant Drosophila Ultrabithorax lacking the first 26 aa from N-terminus fusion protein
Alternate Gene Names: CG10388, bx, Cbx, pbx, abx, bxd, DmUbx
Alternate Antibody Name: FP6.87, anti-UbdA
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 350-363 (Uniprot# P83949-1) region contains at least part of the epitope.
Uniprot ID: P83949 P29555 
Immunogen Sequence: Partial protein
Entrez Gene ID: 42034 42037 
Additional Characterization:  
Antibody Registry ID: AB_10660834 
Additional Information: This antibody cross-reacts with abdominal-A and recognizes all isoforms of the two proteins. Based on sequence homology, this antibody should also work in Aedes, Bombyx, Apis, and Tribolium [PMID 7514518].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ubx/ABD-A FP6.87 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Ubx/ABD-A FP6.87)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins.
    Akam M
    Developmental genetics 15.1 (1994): 19-31.

    Crustacean appendage evolution associated with changes in Hox gene expression.
    Patel NH
    Nature 388.6643 (1997 Aug 14): 682-6.

    Functional evolution of the Ultrabithorax protein.
    Carroll SB
    Proceedings of the National Academy of Sciences of the United States of America 97.2 (2000 Jan 18): 704-9.

    A role of Ultrabithorax in morphological differences between Drosophila species.
    Stern DL
    Nature 396.6710 (1998 Dec 3): 463-6.

    Evolution of the entire arthropod Hox gene set predated the origin and radiation of the onychophoran/arthropod clade.
    Carroll S
    Current biology : CB 7.8 (1997 Aug 1): 547-53.

    Developmental origin and evolution of bacteriocytes in the aphid-Buchnera symbiosis.
    Stern DL
    PLoS biology 1.1 (2003 Oct): E21.

    Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.
    Tsuda L
    Genes to cells : devoted to molecular & cellular mechanisms 18.11 (2013 Nov): 909-20.

    Knockdown of Parhyale Ultrabithorax recapitulates evolutionary changes in crustacean appendage morphology.
    Patel NH
    Proceedings of the National Academy of Sciences of the United States of America 106.33 (2009 Aug 18): 13892-6.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
    Stathopoulos A
    Developmental cell 47.6 (2018 Dec 17): 773-784.e6.

    IHC References
    Epitope Map References
    All References

    Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins.
    Akam M
    Developmental genetics 15.1 (1994): 19-31.

    Crustacean appendage evolution associated with changes in Hox gene expression.
    Patel NH
    Nature 388.6643 (1997 Aug 14): 682-6.

    Comparative functional analyses of ultrabithorax reveal multiple steps and paths to diversification of legs in the adaptive radiation of semi-aquatic insects.
    Rowe L
    Evolution; international journal of organic evolution 68.8 (2014 Aug): 2159-70.

    Homeotic gene expression in the wild-type and a homeotic mutant of the moth Manduca sexta.
    Booker R
    Development genes and evolution 209.8 (1999 Aug): 460-72.

    A conserved mode of head segmentation in arthropods revealed by the expression pattern of Hox genes in a spider.
    Tautz D
    Proceedings of the National Academy of Sciences of the United States of America 95.18 (1998 Sep 1): 10665-70.

    Evolution of a novel appendage ground plan in water striders is driven by changes in the Hox gene Ultrabithorax.
    Rowe L
    PLoS genetics 5.7 (2009 Jul): e1000583.

    Functional evolution of the Ultrabithorax protein.
    Carroll SB
    Proceedings of the National Academy of Sciences of the United States of America 97.2 (2000 Jan 18): 704-9.

    A role of Ultrabithorax in morphological differences between Drosophila species.
    Stern DL
    Nature 396.6710 (1998 Dec 3): 463-6.

    Evolution of the entire arthropod Hox gene set predated the origin and radiation of the onychophoran/arthropod clade.
    Carroll S
    Current biology : CB 7.8 (1997 Aug 1): 547-53.

    Developmental origin and evolution of bacteriocytes in the aphid-Buchnera symbiosis.
    Stern DL
    PLoS biology 1.1 (2003 Oct): E21.

    Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.
    Tsuda L
    Genes to cells : devoted to molecular & cellular mechanisms 18.11 (2013 Nov): 909-20.

    Knockdown of Parhyale Ultrabithorax recapitulates evolutionary changes in crustacean appendage morphology.
    Patel NH
    Proceedings of the National Academy of Sciences of the United States of America 106.33 (2009 Aug 18): 13892-6.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
    Stathopoulos A
    Developmental cell 47.6 (2018 Dec 17): 773-784.e6.

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