anti-Relish-C 21F3

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$40.00
SKU: anti-Relish-C 21F3
View product citations for antibody anti-Relish-C 21F3 on CiteAb

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Available: 270

DSHB Data Sheet

Catalog Fields

Antigen: Relish/NF-kappaB
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Stoven, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Umea University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is an important regulator of innate immunity in insects.
Antigen Molecular Weight: ~110 kDa/ ~100 kDa maternal relish
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rel
Immunogen: Synthetic peptide conjugated to KLH
Alternate Gene Names: CG11992
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: C-terminus
Uniprot ID: Q94527 
Immunogen Sequence: C-terminal PLGHGSDPQDRKWM
Entrez Gene ID: 41087 
Additional Characterization:  
Antibody Registry ID: AB_1553772 
Additional Information: FBgg0000480 / RRID:AB_1553772
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Relish-C 21F3 was deposited to the DSHB by Stoven, S. (DSHB Hybridoma Product anti-Relish-C 21F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Activation of the Drosophila NF-kappaB factor Relish by rapid endoproteolytic cleavage.
    Hultmark D
    EMBO reports 1.4 (2000 Oct): 347-52.

    Caspase-mediated processing of the Drosophila NF-kappaB factor Relish.
    Hultmark D
    Proceedings of the National Academy of Sciences of the United States of America 100.10 (2003 May 13): 5991-6.

    IHC References
    IP References
    Epitope Map References
    All References

    Activation of the Drosophila NF-kappaB factor Relish by rapid endoproteolytic cleavage.
    Hultmark D
    EMBO reports 1.4 (2000 Oct): 347-52.

    Caspase-mediated processing of the Drosophila NF-kappaB factor Relish.
    Hultmark D
    Proceedings of the National Academy of Sciences of the United States of America 100.10 (2003 May 13): 5991-6.

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