anti-dorsal 7A4
Catalog Fields
Antigen: Dorsal, embryonic polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Steward, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Rutgers University, Waksman Institute
Positive Tested Species Reactivity: Drosophila
Depositors Notes: MAb 7a4 recognizes the C-terminal region of dorsal (404-677) of isoform A (D). This sequence is not shared with isoform C (F).
Predicted Species Reactivity:
Gene: dl
Immunogen: Recombinant fusion protein (isoform A or D).
Alternate Gene Names: Dmel\CG6667; NFkappaB;
Alternate Antibody Name: anti-dorsal; anti-dl; 7A4-39
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 404-677
Uniprot ID: E1JHK3
Immunogen Sequence: Full length protein
Entrez Gene ID: 35047
Antibody Registry ID: AB_528204
Additional Information: Dorsal is the homolog of the mammalian NF-KappaB (homology to NF-KappaB in the N-terminal region of dorsal not recognized by the mAb 7A4).
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-dorsal 7A4 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-dorsal 7A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
21 References
Initial Publication
Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal.
Steward R
The Journal of cell biology 123.3 (1993 Nov): 523-34.