anti-dorsal 7A4

(0) No Reviews yet
$40.00
SKU: anti-dorsal 7A4
View product citations for antibody anti-dorsal 7A4 on CiteAb

In Stock

Available: 251

DSHB Data Sheet

Catalog Fields

Antigen: Dorsal, embryonic polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Steward, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Rutgers University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: MAb 7a4 recognizes the C-terminal region of dorsal (404-677) of isoform A (D). This sequence is not shared with isoform C (F).
Antigen Molecular Weight: Predicted: 75kDa; Apparent: 85 to 90kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: dl
Immunogen: Recombinant fusion protein (isoform A or D).
Alternate Gene Names: Dmel\CG6667; NFkappaB;
Alternate Antibody Name: anti-dorsal; anti-dl; 7A4-39
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 404-677
Uniprot ID: E1JHK3 
Immunogen Sequence: Full length protein
Entrez Gene ID: 35047 
Additional Characterization:  
Antibody Registry ID: AB_528204 
Additional Information: Dorsal is the homolog of the mammalian NF-KappaB (homology to NF-KappaB in the N-terminal region of dorsal not recognized by the mAb 7A4).
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-dorsal 7A4 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-dorsal 7A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31.
    Sibon OC
    Developmental biology 313.2 (2008 Jan 15): 802-13.

    Graded maternal short gastrulation protein contributes to embryonic dorsal-ventral patterning by delayed induction.
    Araujo H
    Developmental biology 296.1 (2006 Aug 1): 203-18.

    Dynamics of the Dorsal morphogen gradient.
    Shvartsman SY
    Proceedings of the National Academy of Sciences of the United States of America 106.51 (2009 Dec 22): 21707-12.

    Persephone/Spätzle pathogen sensors mediate the activation of Toll receptor signaling in response to endogenous danger signals in apoptosis-deficient Drosophila.
    Miura M
    The Journal of biological chemistry 289.11 (2014 Mar 14): 7558-68.

    Hemolymph-dependent and -independent responses in Drosophila immune tissue.
    Ip YT
    Journal of cellular biochemistry 92.4 (2004 Jul 1): 849-63.

    Plasma membrane polarity and compartmentalization are established before cellularization in the fly embryo.
    Lippincott-Schwartz J
    Developmental cell 16.1 (2009 Jan): 93-104.

    Retromer promotes immune quiescence by suppressing Spätzle-Toll pathway in Drosophila.
    Lin X
    Journal of cellular physiology 229.4 (2014 Apr): 512-520.

    A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting.
    van Meyel DJ
    Genetics 176.4 (2007 Aug): 2247-63.

    Drosophila WntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo.
    Ip YT
    Development (Cambridge, England) 132.15 (2005 Aug): 3419-29.

    Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae.
    Govind S
    PLoS pathogens 6.12 (2010 Dec 23): e1001234.

    New gene evolution in the bonus-TIF1-γ/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network.
    Newfeld SJ
    Molecular biology and evolution 31.9 (2014 Sep): 2309-21.

    Calpain A modulates Toll responses by limited Cactus/IκB proteolysis.
    Araujo H
    Molecular biology of the cell 24.18 (2013 Sep): 2966-80.

    Quantitative imaging of the Dorsal nuclear gradient reveals limitations to threshold-dependent patterning in Drosophila.
    Stathopoulos A
    Proceedings of the National Academy of Sciences of the United States of America 106.52 (2009 Dec 29): 22317-22.

    Lipid-independent secretion of a Drosophila Wnt protein.
    Nusse R
    The Journal of biological chemistry 283.25 (2008 Jun 20): 17092-8.

    Polydnaviral ankyrin proteins aid parasitic wasp survival by coordinate and selective inhibition of hematopoietic and immune NF-kappa B signaling in insect hosts.
    Govind S
    PLoS pathogens 9.8 (2013): e1003580.

    Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis.
    Steward R
    Molecular and cellular biology 16.3 (1996 Mar): 1103-14.

    Mechanism and implications of morphogen shuttling: Lessons learned from dorsal and Cactus in Drosophila.
    Reeves GT
    Developmental biology 461.1 (2020 May 1): 13-18.

    WB References
    IHC References
    IP References
    Epitope Map References
    All References

    A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting.
    van Meyel DJ
    Genetics 176.4 (2007 Aug): 2247-63.

    Notch and PKC are involved in formation of the lateral region of the dorso-ventral axis in Drosophila embryos.
    Wesley CS
    PloS one 8.7 (2013): e67789.

    Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31.
    Sibon OC
    Developmental biology 313.2 (2008 Jan 15): 802-13.

    Graded maternal short gastrulation protein contributes to embryonic dorsal-ventral patterning by delayed induction.
    Araujo H
    Developmental biology 296.1 (2006 Aug 1): 203-18.

    Dynamics of the Dorsal morphogen gradient.
    Shvartsman SY
    Proceedings of the National Academy of Sciences of the United States of America 106.51 (2009 Dec 22): 21707-12.

    Persephone/Spätzle pathogen sensors mediate the activation of Toll receptor signaling in response to endogenous danger signals in apoptosis-deficient Drosophila.
    Miura M
    The Journal of biological chemistry 289.11 (2014 Mar 14): 7558-68.

    Hemolymph-dependent and -independent responses in Drosophila immune tissue.
    Ip YT
    Journal of cellular biochemistry 92.4 (2004 Jul 1): 849-63.

    Plasma membrane polarity and compartmentalization are established before cellularization in the fly embryo.
    Lippincott-Schwartz J
    Developmental cell 16.1 (2009 Jan): 93-104.

    Retromer promotes immune quiescence by suppressing Spätzle-Toll pathway in Drosophila.
    Lin X
    Journal of cellular physiology 229.4 (2014 Apr): 512-520.

    Drosophila WntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo.
    Ip YT
    Development (Cambridge, England) 132.15 (2005 Aug): 3419-29.

    Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae.
    Govind S
    PLoS pathogens 6.12 (2010 Dec 23): e1001234.

    New gene evolution in the bonus-TIF1-γ/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network.
    Newfeld SJ
    Molecular biology and evolution 31.9 (2014 Sep): 2309-21.

    Calpain A modulates Toll responses by limited Cactus/IκB proteolysis.
    Araujo H
    Molecular biology of the cell 24.18 (2013 Sep): 2966-80.

    Quantitative imaging of the Dorsal nuclear gradient reveals limitations to threshold-dependent patterning in Drosophila.
    Stathopoulos A
    Proceedings of the National Academy of Sciences of the United States of America 106.52 (2009 Dec 29): 22317-22.

    Lipid-independent secretion of a Drosophila Wnt protein.
    Nusse R
    The Journal of biological chemistry 283.25 (2008 Jun 20): 17092-8.

    Polydnaviral ankyrin proteins aid parasitic wasp survival by coordinate and selective inhibition of hematopoietic and immune NF-kappa B signaling in insect hosts.
    Govind S
    PLoS pathogens 9.8 (2013): e1003580.

    Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis.
    Steward R
    Molecular and cellular biology 16.3 (1996 Mar): 1103-14.

    Mechanism and implications of morphogen shuttling: Lessons learned from dorsal and Cactus in Drosophila.
    Reeves GT
    Developmental biology 461.1 (2020 May 1): 13-18.

    Toll-6 and Toll-7 function as neurotrophin receptors in the Drosophila melanogaster CNS.
    Hidalgo A
    Nature neuroscience 16.9 (2013 Sep): 1248-56.

    Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal.
    Steward R
    The Journal of cell biology 123.3 (1993 Nov): 523-34.

    Complex interactions between cis-regulatory modules in native conformation are critical for Drosophila snail expression.
    Stathopoulos A
    Development (Cambridge, England) 138.18 (2011 Sep): 4075-84.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review