anti-cactus 3H12

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SKU: anti-cactus 3H12

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DSHB Data Sheet

Catalog Fields

Product Name/ID: anti-cactus 3H12
Available to For-Profits: Yes
Alternate Antibody Name: 3H12-29
Gene Name: cact
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528109 
Uniprot ID: Q03017 
Entrez Gene ID: 34969 
Clonality: Monoclonal
Immunogen: Recombinant GST/cactus fusion protein
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: cactus
Epitope Location or Sequence: C-terminal (aa 262-458)
Alternate Antigen Name:
Deposit Date: 3/20/2003
Antigen Molecular Weight: Predicted: 52 and 54 kDa; Apparent: 69, 71, and 72 kDa
Depositor: Steward, R.
Antigen Sequence:
Depositor Institution: Rutgers University, Waksman Institute
Antigen Species: Drosophila melanogaster
Depositor Notes: Works for immunoprecipitation, but not very well.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Interaction of dorsal with cactus appears to make the 3H12 epitope inaccessible. 3H12 recognizes all isoforms.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-cactus 3H12 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-cactus 3H12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    Epitope Map References
    All References

    Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal.
    Steward R
    The Journal of cell biology 123.3 (1993 Nov): 523-34.

    The Ca2+-dependent protease Calpain A regulates Cactus/I kappaB levels during Drosophila development in response to maternal Dpp signals.
    Araujo H
    Mechanisms of development 126.8-9 (2009 Aug-Sep): 737-51.

    Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae.
    Govind S
    PLoS pathogens 6.12 (2010 Dec 23): e1001234.

    A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting.
    van Meyel DJ
    Genetics 176.4 (2007 Aug): 2247-63.

    Calpain A modulates Toll responses by limited Cactus/IκB proteolysis.
    Araujo H
    Molecular biology of the cell 24.18 (2013 Sep): 2966-80.

    Notch and PKC are involved in formation of the lateral region of the dorso-ventral axis in Drosophila embryos.
    Wesley CS
    PloS one 8.7 (2013): e67789.

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis.
    Steward R
    Molecular and cellular biology 16.3 (1996 Mar): 1103-14.

    Toll-6 and Toll-7 function as neurotrophin receptors in the Drosophila melanogaster CNS.
    Hidalgo A
    Nature neuroscience 16.9 (2013 Sep): 1248-56.

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