anti-cactus 3H12

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$40.00
SKU: anti-cactus 3H12
View product citations for antibody anti-cactus 3H12 on CiteAb

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Available: 131

DSHB Data Sheet

Catalog Fields

Antigen: cactus
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Steward, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Waksman Institute, Rutgers University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Works for immunoprecipitation, but not very well.
Antigen Molecular Weight: Predicted: 52 and 54 kDa; Apparent: 69, 71, and 72 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cact
Immunogen: Recombinant GST/cactus fusion protein
Alternate Gene Names: CG5848, cac, dip6, IkappaB
Alternate Antibody Name: 3H12-29
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: C-terminal (aa 262-458)
Uniprot ID: Q03017 
Immunogen Sequence: Full length protein
Entrez Gene ID: 34969 
Additional Characterization:  
Antibody Registry ID: AB_528109 
Additional Information: Interaction of dorsal with cactus appears to make the 3H12 epitope inaccessible. 3H12 recognizes all isoforms.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-cactus 3H12 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-cactus 3H12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    Epitope Map References
    All References

    Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal.
    Steward R
    The Journal of cell biology 123.3 (1993 Nov): 523-34.

    The Ca2+-dependent protease Calpain A regulates Cactus/I kappaB levels during Drosophila development in response to maternal Dpp signals.
    Araujo H
    Mechanisms of development 126.8-9 (2009 Aug-Sep): 737-51.

    Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae.
    Govind S
    PLoS pathogens 6.12 (2010 Dec 23): e1001234.

    A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting.
    van Meyel DJ
    Genetics 176.4 (2007 Aug): 2247-63.

    Calpain A modulates Toll responses by limited Cactus/IκB proteolysis.
    Araujo H
    Molecular biology of the cell 24.18 (2013 Sep): 2966-80.

    Notch and PKC are involved in formation of the lateral region of the dorso-ventral axis in Drosophila embryos.
    Wesley CS
    PloS one 8.7 (2013): e67789.

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis.
    Steward R
    Molecular and cellular biology 16.3 (1996 Mar): 1103-14.

    Toll-6 and Toll-7 function as neurotrophin receptors in the Drosophila melanogaster CNS.
    Hidalgo A
    Nature neuroscience 16.9 (2013 Sep): 1248-56.

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