anti-cactus 3H12
Catalog Fields
Antigen: cactus
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Steward, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Waksman Institute, Rutgers University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Works for immunoprecipitation, but not very well.
Antigen Molecular Weight: Predicted: 52 and 54 kDa; Apparent: 69, 71, and 72 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: cact
Immunogen: Recombinant GST/cactus fusion protein
Alternate Gene Names: CG5848, cac, dip6, IkappaB
Alternate Antibody Name: 3H12-29
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: C-terminal (aa 262-458)
Uniprot ID: Q03017
Immunogen Sequence: Full length protein
Entrez Gene ID: 34969
Additional Characterization:
Antibody Registry ID: AB_528109
Additional Information: Interaction of dorsal with cactus appears to make the 3H12 epitope inaccessible. 3H12 recognizes all isoforms.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-cactus 3H12 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-cactus 3H12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal.
Steward R
The Journal of cell biology 123.3 (1993 Nov): 523-34.