anti-achaete

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$40.00
SKU: anti-achaete
View product citations for antibody anti-achaete on CiteAb

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Available: 86

DSHB Data Sheet

Catalog Fields

Antigen: Achaete protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Skeath, J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University, St. Louis
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This monoclonal antibody is also known as 990ESF1.
Antigen Molecular Weight: 22.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ac
Immunogen: Achaete protein domain
Alternate Gene Names: Dmel_CG3796
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: Q24639 
Immunogen Sequence: 178 a.a.
Entrez Gene ID: 30981 
Additional Characterization:  
Antibody Registry ID: AB_528066 
Additional Information: FBgn0012861. RRID: AB_528066
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-achaete was deposited to the DSHB by Skeath, J. (DSHB Hybridoma Product anti-achaete)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling.
    Schejter ED
    The Journal of cell biology 152.1 (2001 Jan 8): 1-13.

    Cullin-3 regulates pattern formation, external sensory organ development and cell survival during Drosophila development.
    Skeath JB
    Mechanisms of development 121.12 (2004 Dec): 1495-507.

    Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing.
    Truman JW
    Development (Cambridge, England) 132.23 (2005 Dec): 5239-48.

    A mosaic genetic screen for novel mutations affecting Drosophila neuroblast divisions.
    Overton PM
    BMC genetics 7. (2006 Jun 2): 33.

    NF-kappaB/Rel-mediated regulation of the neural fate in Drosophila.
    Simpson P
    PloS one 2.11 (2007 Nov 14): e1178.

    Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling.
    Freeman M
    Development (Cambridge, England) 136.3 (2009 Feb): 473-81.

    A screen for modifiers of notch signaling uncovers Amun, a protein with a critical role in sensory organ development.
    Muskavitch MA
    Genetics 182.4 (2009 Aug): 1061-76.

    The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila.
    Newfeld SJ
    PloS one 5.7 (2010 Jul 16): e11619.

    Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation.
    Nakato H
    Developmental biology 345.2 (2010 Sep 15): 204-14.

    Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster.
    Vaccari T
    Autophagy 12.3 (2016): 499-514.

    IHC References
    All References

    Gene regulation in two dimensions: the proneural achaete and scute genes are controlled by combinations of axis-patterning genes through a common intergenic control region.
    Carroll SB
    Genes & development 6.12B (1992 Dec): 2606-19.

    Regulation of achaete-scute gene expression and sensory organ pattern formation in the Drosophila wing.
    Carroll SB
    Genes & development 5.6 (1991 Jun): 984-95.

    Regulation of proneural gene expression and cell fate during neuroblast segregation in the Drosophila embryo.
    Carroll SB
    Development (Cambridge, England) 114.4 (1992 Apr): 939-46.

    Identifying targets of the Sox domain protein Dichaete in the Drosophila CNS via targeted expression of dominant negative proteins.
    Russell S
    BMC developmental biology 13. (2013 Jan 5): 1.

    Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling.
    Schejter ED
    The Journal of cell biology 152.1 (2001 Jan 8): 1-13.

    Cullin-3 regulates pattern formation, external sensory organ development and cell survival during Drosophila development.
    Skeath JB
    Mechanisms of development 121.12 (2004 Dec): 1495-507.

    Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing.
    Truman JW
    Development (Cambridge, England) 132.23 (2005 Dec): 5239-48.

    A mosaic genetic screen for novel mutations affecting Drosophila neuroblast divisions.
    Overton PM
    BMC genetics 7. (2006 Jun 2): 33.

    NF-kappaB/Rel-mediated regulation of the neural fate in Drosophila.
    Simpson P
    PloS one 2.11 (2007 Nov 14): e1178.

    Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling.
    Freeman M
    Development (Cambridge, England) 136.3 (2009 Feb): 473-81.

    A screen for modifiers of notch signaling uncovers Amun, a protein with a critical role in sensory organ development.
    Muskavitch MA
    Genetics 182.4 (2009 Aug): 1061-76.

    The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila.
    Newfeld SJ
    PloS one 5.7 (2010 Jul 16): e11619.

    Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation.
    Nakato H
    Developmental biology 345.2 (2010 Sep 15): 204-14.

    Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster.
    Vaccari T
    Autophagy 12.3 (2016): 499-514.

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