anti-dFmr1, 5A11

(0) No Reviews yet
$40.00
SKU: anti-dFmr1, 5A11
View product citations for antibody anti-dFmr1, 5A11 on CiteAb

In Stock

Available: 193

DSHB Data Sheet

Catalog Fields

Antigen: dFmr1 protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Siomi, H.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Keio University School of Medicine
Positive Tested Species Reactivity: Drosophila
Depositors Notes: MW: 78 kDa (+82 kDa minor band)
Antigen Molecular Weight: Multiple isforms; Predicted: between 60 and 76 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Fmr1
Immunogen: Recombinant His-tagged dFmr1 protein
Alternate Gene Names: CG6203, dFMRP, dFXR1, dFXRP
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q9NFU0 
Immunogen Sequence: Full length protein
Entrez Gene ID: 37528 
Additional Characterization:
Antibody Registry ID: AB_528252 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-dFmr1, 5A11 was deposited to the DSHB by Siomi, H. (DSHB Hybridoma Product anti-dFmr1, 5A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways.
    Siomi MC
    Genes and development 18.14 (2004 Jul 15): 1655-66.

    IF References

    Short- and long-term memory are modulated by multiple isoforms of the fragile X mental retardation protein.
    Dockendorff TC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.19 (2010 May 12): 6782-92.

    Motility screen identifies Drosophila IGF-II mRNA-binding protein--zipcode-binding protein acting in oogenesis and synaptogenesis.
    Hays TS
    PLoS genetics 4.2 (2008 Feb): e36.

    The Drosophila fragile X mental retardation protein participates in the piRNA pathway.
    Giangrande A
    Journal of cell science 128.11 (2015 Jun 1): 2070-84.

    TORC2 mediates the heat stress response in Drosophila by promoting the formation of stress granules.
    Rabouille C
    Journal of cell science 128.14 (2015 Jul 15): 2497-508.

    A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation.
    Rabouille C
    eLife 3. (2014 Nov 11): .

    FMRP and Ataxin-2 function together in long-term olfactory habituation and neuronal translational control.
    Ramaswami M
    Proceedings of the National Academy of Sciences of the United States of America 111.1 (2014 Jan 7): E99-E108.

    Bicaudal-D regulates fragile X mental retardation protein levels, motility, and function during neuronal morphogenesis.
    Bullock SL
    Current biology : CB 20.16 (2010 Aug 24): 1487-92.

    Mutational analysis establishes a critical role for the N terminus of fragile X mental retardation protein FMRP.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.12 (2008 Mar 19): 3221-6.

    WB References

    Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways.
    Siomi MC
    Genes and development 18.14 (2004 Jul 15): 1655-66.

    The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila.
    Gao FB
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.46 (2008 Nov 12): 11883-9.

    Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects.
    Jongens TA
    PloS one 4.10 (2009 Oct 27): e7618.

    Circadian phenotypes of Drosophila fragile x mutants in alternative genetic backgrounds.
    Shimoda M
    Zoological science 25.6 (2008 Jun): 561-71.

    Short- and long-term memory are modulated by multiple isoforms of the fragile X mental retardation protein.
    Dockendorff TC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.19 (2010 May 12): 6782-92.

    Substitution of critical isoleucines in the KH domains of Drosophila fragile X protein results in partial loss-of-function phenotypes.
    Dockendorff TC
    Genetics 175.3 (2007 Mar): 1241-50.

    Motility screen identifies Drosophila IGF-II mRNA-binding protein--zipcode-binding protein acting in oogenesis and synaptogenesis.
    Hays TS
    PLoS genetics 4.2 (2008 Feb): e36.

    The Drosophila fragile X mental retardation protein participates in the piRNA pathway.
    Giangrande A
    Journal of cell science 128.11 (2015 Jun 1): 2070-84.

    Bicaudal-D regulates fragile X mental retardation protein levels, motility, and function during neuronal morphogenesis.
    Bullock SL
    Current biology : CB 20.16 (2010 Aug 24): 1487-92.

    Mutational analysis establishes a critical role for the N terminus of fragile X mental retardation protein FMRP.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.12 (2008 Mar 19): 3221-6.

    IP References
    All References

    Short- and long-term memory are modulated by multiple isoforms of the fragile X mental retardation protein.
    Dockendorff TC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.19 (2010 May 12): 6782-92.

    Motility screen identifies Drosophila IGF-II mRNA-binding protein--zipcode-binding protein acting in oogenesis and synaptogenesis.
    Hays TS
    PLoS genetics 4.2 (2008 Feb): e36.

    The Drosophila fragile X mental retardation protein participates in the piRNA pathway.
    Giangrande A
    Journal of cell science 128.11 (2015 Jun 1): 2070-84.

    TORC2 mediates the heat stress response in Drosophila by promoting the formation of stress granules.
    Rabouille C
    Journal of cell science 128.14 (2015 Jul 15): 2497-508.

    A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation.
    Rabouille C
    eLife 3. (2014 Nov 11): .

    FMRP and Ataxin-2 function together in long-term olfactory habituation and neuronal translational control.
    Ramaswami M
    Proceedings of the National Academy of Sciences of the United States of America 111.1 (2014 Jan 7): E99-E108.

    Bicaudal-D regulates fragile X mental retardation protein levels, motility, and function during neuronal morphogenesis.
    Bullock SL
    Current biology : CB 20.16 (2010 Aug 24): 1487-92.

    Mutational analysis establishes a critical role for the N terminus of fragile X mental retardation protein FMRP.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.12 (2008 Mar 19): 3221-6.

    Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways.
    Siomi MC
    Genes and development 18.14 (2004 Jul 15): 1655-66.

    The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila.
    Gao FB
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.46 (2008 Nov 12): 11883-9.

    Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects.
    Jongens TA
    PloS one 4.10 (2009 Oct 27): e7618.

    Circadian phenotypes of Drosophila fragile x mutants in alternative genetic backgrounds.
    Shimoda M
    Zoological science 25.6 (2008 Jun): 561-71.

    Substitution of critical isoleucines in the KH domains of Drosophila fragile X protein results in partial loss-of-function phenotypes.
    Dockendorff TC
    Genetics 175.3 (2007 Mar): 1241-50.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review