anti-eyeless
Catalog Fields
Product Name/ID: anti-eyeless
Available to For-Profits: Yes
Alternate Antibody Name: anti-Ey
Gene Name: ey
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2253542
Uniprot ID: O18381
Entrez Gene ID: 43812
Clonality: Monoclonal
Immunogen: Recombinant protein fragment (residues 226-470 (the linker region) of the Eyeless protein, transcript variant D)
Clone:
Immunogen Sequence: Residues 226-470 of transcript variant D correspond to 185-429 on the canonical Larval isoform [Uniprot O18381]
Myeloma Strain: SP2OAg14
Epitope Mapped: Yes
Antigen Name: Eyeless protein, linker region
Epitope Location or Sequence: a.a. 185-429
Alternate Antigen Name:
Deposit Date: 8/12/2009
Antigen Molecular Weight: Predicted: 47-48kDa
Depositor: Callaerts, P.
Antigen Sequence:
Depositor Institution: KU Leuven, VIB Lab of Behavioral & Developmental Genetics
Antigen Species: Drosophila
Depositor Notes: The depositor indicates this antibody works in western blot applications.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila melanogaster
Additional Information: The epitope is not in the Paired DNA binding domain.
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-eyeless was deposited to the DSHB by Callaerts, P. (DSHB Hybridoma Product anti-eyeless)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons.
Callaerts P
Proceedings of the National Academy of Sciences of the United States of America 105.42 (2008 Oct 21): 16183-8.