Antigen: Eyeless protein, linker region
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Callaerts, P.
Host Species: mouse
Depositors Institution: VIB Lab of Behavioral & Developmental Genetics, KU Leuven
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: The depositor indicates this antibody works in western blot applications.
Antigen Molecular Weight: Predicted: 47-48kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant protein fragment (residues 226-470 (the linker region) of the Eyeless protein, transcript variant D)
Alternate Gene Names: Dmel\CG1464; DPax-6; EYEL; OK107; Pax6
Alternate Antibody Name: anti-Ey
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2OAg14
Epitope Location or Sequence: a.a. 185-429
Uniprot ID: O18381
Immunogen Sequence: Residues 226-470 of transcript variant D correspond to 185-429 on the canonical Larval isoform [Uniprot O18381]
Entrez Gene ID: 43812
Antibody Registry ID: AB_2253542
Additional Information: The epitope is not in the Paired DNA binding domain.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-eyeless was deposited to the DSHB by Callaerts, P. (DSHB Hybridoma Product anti-eyeless)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References