anti-eyeless

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SKU: anti-eyeless
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DSHB Data Sheet

Catalog Fields

Antigen: Eyeless protein, linker region
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Callaerts, P.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: VIB Lab of Behavioral & Developmental Genetics, KU Leuven
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: The depositor indicates this antibody works in western blot applications.
Antigen Molecular Weight: Predicted: 47-48kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ey
Immunogen: Recombinant protein fragment (residues 226-470 (the linker region) of the Eyeless protein, transcript variant D)
Alternate Gene Names: Dmel\CG1464; DPax-6; EYEL; OK107; Pax6
Alternate Antibody Name: anti-Ey
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2OAg14
Epitope Location or Sequence: a.a. 185-429
Uniprot ID: O18381 
Immunogen Sequence: Residues 226-470 of transcript variant D correspond to 185-429 on the canonical Larval isoform [Uniprot O18381]
Entrez Gene ID: 43812 
Additional Characterization:  
Antibody Registry ID: AB_2253542 
Additional Information: The epitope is not in the Paired DNA binding domain.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-eyeless was deposited to the DSHB by Callaerts, P. (DSHB Hybridoma Product anti-eyeless)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • Epitope Map References
  • All References
  • Initial Publication

    Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons.
    Callaerts P
    Proceedings of the National Academy of Sciences of the United States of America 105.42 (2008 Oct 21): 16183-8.

    IF References

    Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons.
    Callaerts P
    Proceedings of the National Academy of Sciences of the United States of America 105.42 (2008 Oct 21): 16183-8.

    Hipk promotes photoreceptor differentiation through the repression of Twin of eyeless and Eyeless expression.
    Verheyen EM
    Developmental biology 390.1 (2014 Jun 1): 14-25.

    Regulation of ocellar specification and size by twin of eyeless and homothorax.
    Casares F
    Developmental dynamics : an official publication of the American Association of Anatomists 240.1 (2011 Jan): 75-85.

    Conserved role for the Dachshund protein with Drosophila Pax6 homolog Eyeless in insulin expression.
    Nishimura T
    Proceedings of the National Academy of Sciences of the United States of America 109.7 (2012 Feb 14): 2406-11.

    Cell migration in Drosophila optic lobe neurons is controlled by eyeless/Pax6.
    Desplan C
    Development (Cambridge, England) 138.4 (2011 Feb): 687-93.

    Temporal patterning of Drosophila medulla neuroblasts controls neural fates.
    Desplan C
    Nature 498.7455 (2013 Jun 27): 456-62.

    SWI/SNF complex prevents lineage reversion and induces temporal patterning in neural stem cells.
    Knoblich JA
    Cell 156.6 (2014 Mar 13): 1259-1273.

    hth maintains the pool of eye progenitors and its downregulation by Dpp and Hh couples retinal fate acquisition with cell cycle exit.
    Casares F
    Developmental biology 339.1 (2010 Mar 1): 78-88.

    Dual transcriptional activities of SIX proteins define their roles in normal and ectopic eye development.
    Kumar JP
    Development (Cambridge, England) 139.5 (2012 Mar): 991-1000.

    A temporal mechanism that produces neuronal diversity in the Drosophila visual center.
    Sato M
    Developmental biology 380.1 (2013 Aug 1): 12-24.

    The BTB-zinc finger transcription factor abrupt acts as an epithelial oncogene in Drosophila melanogaster through maintaining a progenitor-like cell state.
    Brumby AM
    PLoS genetics 9.7 (2013): e1003627.

    Protection of neuronal diversity at the expense of neuronal numbers during nutrient restriction in the Drosophila visual system.
    Maurange C
    Cell reports 3.3 (2013 Mar 28): 587-94.

    Temporal patterning of neuroblasts controls Notch-mediated cell survival through regulation of Hid or Reaper.
    Desplan C
    Cell 158.5 (2014 Aug 28): 1173-1186.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Epitope Map References

    Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons.
    Callaerts P
    Proceedings of the National Academy of Sciences of the United States of America 105.42 (2008 Oct 21): 16183-8.

    All References

    Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons.
    Callaerts P
    Proceedings of the National Academy of Sciences of the United States of America 105.42 (2008 Oct 21): 16183-8.

    Hipk promotes photoreceptor differentiation through the repression of Twin of eyeless and Eyeless expression.
    Verheyen EM
    Developmental biology 390.1 (2014 Jun 1): 14-25.

    Regulation of ocellar specification and size by twin of eyeless and homothorax.
    Casares F
    Developmental dynamics : an official publication of the American Association of Anatomists 240.1 (2011 Jan): 75-85.

    Conserved role for the Dachshund protein with Drosophila Pax6 homolog Eyeless in insulin expression.
    Nishimura T
    Proceedings of the National Academy of Sciences of the United States of America 109.7 (2012 Feb 14): 2406-11.

    Cell migration in Drosophila optic lobe neurons is controlled by eyeless/Pax6.
    Desplan C
    Development (Cambridge, England) 138.4 (2011 Feb): 687-93.

    Temporal patterning of Drosophila medulla neuroblasts controls neural fates.
    Desplan C
    Nature 498.7455 (2013 Jun 27): 456-62.

    SWI/SNF complex prevents lineage reversion and induces temporal patterning in neural stem cells.
    Knoblich JA
    Cell 156.6 (2014 Mar 13): 1259-1273.

    hth maintains the pool of eye progenitors and its downregulation by Dpp and Hh couples retinal fate acquisition with cell cycle exit.
    Casares F
    Developmental biology 339.1 (2010 Mar 1): 78-88.

    Dual transcriptional activities of SIX proteins define their roles in normal and ectopic eye development.
    Kumar JP
    Development (Cambridge, England) 139.5 (2012 Mar): 991-1000.

    A temporal mechanism that produces neuronal diversity in the Drosophila visual center.
    Sato M
    Developmental biology 380.1 (2013 Aug 1): 12-24.

    The BTB-zinc finger transcription factor abrupt acts as an epithelial oncogene in Drosophila melanogaster through maintaining a progenitor-like cell state.
    Brumby AM
    PLoS genetics 9.7 (2013): e1003627.

    Protection of neuronal diversity at the expense of neuronal numbers during nutrient restriction in the Drosophila visual system.
    Maurange C
    Cell reports 3.3 (2013 Mar 28): 587-94.

    Temporal patterning of neuroblasts controls Notch-mediated cell survival through regulation of Hid or Reaper.
    Desplan C
    Cell 158.5 (2014 Aug 28): 1173-1186.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    The Drosophila Wilms׳ Tumor 1-Associating Protein (WTAP) homolog is required for eye development.
    Kumar JP
    Developmental biology 390.2 (2014 Jun 15): 170-80.

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