mAB sca1

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$40.00
SKU: mAB sca1
View product citations for antibody mAB sca1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: scabrous protein, Drosophila
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Baker, N.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Albert Einstein College of Medicine
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Monospecific in immunohistology or immunoblotting
Antigen Molecular Weight: Predicted: 90 kDa; Apparent: 300 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: sca
Immunogen: Recombinant sca-GST fusion protein
Alternate Gene Names: CG17579,
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63-Ag8.653
Epitope Location or Sequence: aa 41-275
Uniprot ID: P21520 
Immunogen Sequence: Full length protein
Entrez Gene ID: 36411 
Additional Characterization:  
Antibody Registry ID: AB_528459 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB sca1 was deposited to the DSHB by Baker, N. (DSHB Hybridoma Product mAB sca1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Scabrous overexpression in the eye affects R3/R4 cell fate specification and inhibits notch signaling.
    Paricio N
    Developmental dynamics : an official publication of the American Association of Anatomists 245.2 (2016 Feb): 166-74.

    A primary role for the epidermal growth factor receptor in ommatidial spacing in the Drosophila eye.
    Freeman M
    Current biology : CB 11.6 (2001 Mar 20): 396-404.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Echinoid limits R8 photoreceptor specification by inhibiting inappropriate EGF receptor signalling within R8 equivalence groups.
    Jarman AP
    Development (Cambridge, England) 130.16 (2003 Aug): 3715-24.

    Scabrous controls ommatidial rotation in the Drosophila compound eye.
    Chien CT
    Developmental cell 3.6 (2002 Dec): 839-50.

    Genetic analysis of hook, a gene required for endocytic trafficking in drosophila.
    Phistry M
    Genetics 151.2 (1999 Feb): 675-84.

    Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta.
    Jarman AP
    Development (Cambridge, England) 130.26 (2003 Dec): 6475-84.

    Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.
    Choi KW
    Developmental biology 308.2 (2007 Aug 15): 322-30.

    Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition.
    Bellen HJ
    Journal of cell science 126.Pt 16 (2013 Aug 15): 3686-96.

    WB References
    IHC References
    IP References

    The scabrous gene encodes a secreted glycoprotein dimer and regulates proneural development in Drosophila eyes.
    Baker NE
    Molecular and cellular biology 16.3 (1996 Mar): 1179-88.

    Delta activity independent of its activity as a ligand of Notch.
    Wesley C
    BMC developmental biology 5. (2005 Mar 10): 6.

    Epitope Map References
    All References

    Nicastrin is required for gamma-secretase cleavage of the Drosophila Notch receptor.
    Fortini ME
    Developmental cell 2.1 (2002 Jan): 69-78.

    Functional analysis of the fibrinogen-related scabrous gene from Drosophila melanogaster identifies potential effector and stimulatory protein domains.
    Baker NE
    Genetics 150.2 (1998 Oct): 663-73.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Scabrous overexpression in the eye affects R3/R4 cell fate specification and inhibits notch signaling.
    Paricio N
    Developmental dynamics : an official publication of the American Association of Anatomists 245.2 (2016 Feb): 166-74.

    A primary role for the epidermal growth factor receptor in ommatidial spacing in the Drosophila eye.
    Freeman M
    Current biology : CB 11.6 (2001 Mar 20): 396-404.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Echinoid limits R8 photoreceptor specification by inhibiting inappropriate EGF receptor signalling within R8 equivalence groups.
    Jarman AP
    Development (Cambridge, England) 130.16 (2003 Aug): 3715-24.

    Scabrous controls ommatidial rotation in the Drosophila compound eye.
    Chien CT
    Developmental cell 3.6 (2002 Dec): 839-50.

    Genetic analysis of hook, a gene required for endocytic trafficking in drosophila.
    Phistry M
    Genetics 151.2 (1999 Feb): 675-84.

    Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta.
    Jarman AP
    Development (Cambridge, England) 130.26 (2003 Dec): 6475-84.

    Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.
    Choi KW
    Developmental biology 308.2 (2007 Aug 15): 322-30.

    Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition.
    Bellen HJ
    Journal of cell science 126.Pt 16 (2013 Aug 15): 3686-96.

    The scabrous gene encodes a secreted glycoprotein dimer and regulates proneural development in Drosophila eyes.
    Baker NE
    Molecular and cellular biology 16.3 (1996 Mar): 1179-88.

    Delta activity independent of its activity as a ligand of Notch.
    Wesley C
    BMC developmental biology 5. (2005 Mar 10): 6.

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