mAB sca1

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SKU: mAB sca1

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: mAB sca1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: sca
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_528459 
Uniprot ID: P21520 
RRID:  
Entrez Gene ID: 36411 
Clonality: Monoclonal
Immunogen: Recombinant sca-GST fusion protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: scabrous protein, Drosophila
Epitope Location or Sequence: aa 41-275
Alternate Antigen Name:
Deposit Date: 8/30/1995
Antigen Molecular Weight: Predicted: 90 kDa; Apparent: 300 kDa
Depositor: Baker, N.
Antigen Sequence:
Depositor Institution: Albert Einstein College of Medicine
Antigen Species: Drosophila
Depositor Notes: Monospecific in immunohistology or immunoblotting
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB sca1 was deposited to the DSHB by Baker, N. (DSHB Hybridoma Product mAB sca1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Scabrous overexpression in the eye affects R3/R4 cell fate specification and inhibits notch signaling.
    Paricio N
    Developmental dynamics : an official publication of the American Association of Anatomists 245.2 (2016 Feb): 166-74.

    A primary role for the epidermal growth factor receptor in ommatidial spacing in the Drosophila eye.
    Freeman M
    Current biology : CB 11.6 (2001 Mar 20): 396-404.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Echinoid limits R8 photoreceptor specification by inhibiting inappropriate EGF receptor signalling within R8 equivalence groups.
    Jarman AP
    Development (Cambridge, England) 130.16 (2003 Aug): 3715-24.

    Scabrous controls ommatidial rotation in the Drosophila compound eye.
    Chien CT
    Developmental cell 3.6 (2002 Dec): 839-50.

    Genetic analysis of hook, a gene required for endocytic trafficking in drosophila.
    Phistry M
    Genetics 151.2 (1999 Feb): 675-84.

    Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta.
    Jarman AP
    Development (Cambridge, England) 130.26 (2003 Dec): 6475-84.

    Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.
    Choi KW
    Developmental biology 308.2 (2007 Aug 15): 322-30.

    Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition.
    Bellen HJ
    Journal of cell science 126.Pt 16 (2013 Aug 15): 3686-96.

    WB References
    IHC References
    IP References

    The scabrous gene encodes a secreted glycoprotein dimer and regulates proneural development in Drosophila eyes.
    Baker NE
    Molecular and cellular biology 16.3 (1996 Mar): 1179-88.

    Delta activity independent of its activity as a ligand of Notch.
    Wesley C
    BMC developmental biology 5. (2005 Mar 10): 6.

    Epitope Map References
    All References

    Nicastrin is required for gamma-secretase cleavage of the Drosophila Notch receptor.
    Fortini ME
    Developmental cell 2.1 (2002 Jan): 69-78.

    Functional analysis of the fibrinogen-related scabrous gene from Drosophila melanogaster identifies potential effector and stimulatory protein domains.
    Baker NE
    Genetics 150.2 (1998 Oct): 663-73.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Scabrous overexpression in the eye affects R3/R4 cell fate specification and inhibits notch signaling.
    Paricio N
    Developmental dynamics : an official publication of the American Association of Anatomists 245.2 (2016 Feb): 166-74.

    A primary role for the epidermal growth factor receptor in ommatidial spacing in the Drosophila eye.
    Freeman M
    Current biology : CB 11.6 (2001 Mar 20): 396-404.

    Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development.
    Rebay I
    Mechanisms of development 124.9-10 (2007 Sep-Oct): 792-806.

    Echinoid limits R8 photoreceptor specification by inhibiting inappropriate EGF receptor signalling within R8 equivalence groups.
    Jarman AP
    Development (Cambridge, England) 130.16 (2003 Aug): 3715-24.

    Scabrous controls ommatidial rotation in the Drosophila compound eye.
    Chien CT
    Developmental cell 3.6 (2002 Dec): 839-50.

    Genetic analysis of hook, a gene required for endocytic trafficking in drosophila.
    Phistry M
    Genetics 151.2 (1999 Feb): 675-84.

    Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta.
    Jarman AP
    Development (Cambridge, England) 130.26 (2003 Dec): 6475-84.

    Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.
    Choi KW
    Developmental biology 308.2 (2007 Aug 15): 322-30.

    Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition.
    Bellen HJ
    Journal of cell science 126.Pt 16 (2013 Aug 15): 3686-96.

    The scabrous gene encodes a secreted glycoprotein dimer and regulates proneural development in Drosophila eyes.
    Baker NE
    Molecular and cellular biology 16.3 (1996 Mar): 1179-88.

    Delta activity independent of its activity as a ligand of Notch.
    Wesley C
    BMC developmental biology 5. (2005 Mar 10): 6.

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