orb2 2D11

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SKU: orb2 2D11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: orb2 2D11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: orb2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619565 
Uniprot ID: Q9VSR3 
RRID:  
Entrez Gene ID: 39018 
Clonality: Monoclonal
Immunogen: Recombinant fusion protein of GST and aa 443-699 (C-terminal) of the 75-kDa isoform of Orb2.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: orb2 protein
Epitope Location or Sequence: C-terminal rrm domains af all isoforms (aa 443-669 of Uniprot ID Q9VSR3-1)
Alternate Antigen Name:
Deposit Date: 7/2/2009
Antigen Molecular Weight: Predicted: 75 kDa; Apparent: 80 kDa
Depositor: Schedl, P.
Antigen Sequence:
Depositor Institution: Princeton University
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: orb2 is a RNA binding protein
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
orb2 2D11 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product orb2 2D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    ELISA References
    Epitope Map References
    All References

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