orb2 2D11

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SKU: orb2 2D11
View product citations for antibody orb2 2D11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: orb2 protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schedl, P.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 75 kDa; Apparent: 80 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: orb2
Immunogen: Recombinant fusion protein of GST and aa 443-699 (C-terminal) of the 75-kDa isoform of Orb2.
Alternate Gene Names: CG43782
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: C-terminal rrm domains af all isoforms (aa 443-669 of Uniprot ID Q9VSR3-1)
Uniprot ID: Q9VSR3 
Immunogen Sequence: Partial protein
Entrez Gene ID: 39018 
Additional Characterization:  
Antibody Registry ID: AB_2619565 
Additional Information: orb2 is a RNA binding protein
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
orb2 2D11 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product orb2 2D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    ELISA References
    Epitope Map References
    All References

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