orb 4H8
Catalog Fields
Antigen: orb protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, lambda light chain
Depositor: Schedl, P.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Princeton University
Antigen Molecular Weight: Multiple isoforms; major bands have an apparent MW between 97 and 100 kDa
Depositors Notes:
Immunogen: Recombinant fusion protein of phage T7 gene Phi10 and Oo18 RNA-binding protein (aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7])
Gene: orb
Alternate Antibody Name:
Alternate Gene Names: CG10868
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: Q9VCN7
Epitope Location or Sequence: aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7], containing the rrm (RNA recognition motif) regions
Entrez Gene ID: 42752
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528418
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: orb is a RNA binding protein
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
orb 4H8 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product orb 4H8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
Schedl P
Genes & development 8.5 (1994 Mar 1): 598-613.