orb 4H8

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$40.00
SKU: orb 4H8
View product citations for antibody orb 4H8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: orb protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schedl, P.
Isotype: MIgG1, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple isoforms; major bands have an apparent MW between 97 and 100 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: orb
Immunogen: Recombinant fusion protein of phage T7 gene Phi10 and Oo18 RNA-binding protein (aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7])
Alternate Gene Names: CG10868
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7], containing the rrm (RNA recognition motif) regions
Uniprot ID: Q9VCN7 
Immunogen Sequence: Partial protein
Entrez Gene ID: 42752 
Additional Characterization:  
Antibody Registry ID: AB_528418 
Additional Information: orb is a RNA binding protein
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
orb 4H8 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product orb 4H8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster.
    Schedl P
    PLoS genetics 8.11 (2012): e1003079.

    The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
    Schedl P
    Genes & development 8.5 (1994 Mar 1): 598-613.

    A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
    St Johnston D
    Nature 421.6921 (2003 Jan 23): 379-84.

    Cup blocks the precocious activation of the orb autoregulatory loop.
    Schedl P
    PloS one 6.12 (2011): e28261.

    The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila.
    McKim KS
    Current biology : CB 13.4 (2003 Feb 18): 276-85.

    Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila.
    González-Reyes A
    PLoS biology 10.4 (2012): e1001298.

    Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage.
    Knoblich JA
    Nature 454.7201 (2008 Jul 10): 241-5.

    Poly(ADP-ribose) controls DE-cadherin-dependent stem cell maintenance and oocyte localization.
    Tulin AV
    Nature communications 3. (2012 Mar 27): 760.

    H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila.
    Kobayashi S
    Biology open 4.2 (2015 Jan 8): 119-24.

    The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway.
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Bicaudal-C recruits CCR4-NOT deadenylase to target mRNAs and regulates oogenesis, cytoskeletal organization, and its own expression.
    Lasko P
    Developmental cell 13.5 (2007 Nov): 691-704.

    Kinase-activity-independent functions of atypical protein kinase C in Drosophila.
    Wodarz A
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3759-71.

    Windei, the Drosophila homolog of mAM/MCAF1, is an essential cofactor of the H3K9 methyl transferase dSETDB1/Eggless in germ line development.
    Wodarz A
    PLoS genetics 5.9 (2009 Sep): e1000644.

    Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis.
    Lasko P
    PloS one 10.4 (2015): e0122519.

    <i>Drosophila</i> Mon1 constitutes a novel node in the brain-gonad axis that is essential for female germline maturation.
    Ratnaparkhi A
    Development (Cambridge, England) 146.13 (2019 Jul 10): .

    WB References
    IP References
    Epitope Map References
    All References

    The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster.
    Schedl P
    PLoS genetics 8.11 (2012): e1003079.

    The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
    Schedl P
    Genes & development 8.5 (1994 Mar 1): 598-613.

    A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
    St Johnston D
    Nature 421.6921 (2003 Jan 23): 379-84.

    Cup blocks the precocious activation of the orb autoregulatory loop.
    Schedl P
    PloS one 6.12 (2011): e28261.

    The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila.
    McKim KS
    Current biology : CB 13.4 (2003 Feb 18): 276-85.

    Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila.
    González-Reyes A
    PLoS biology 10.4 (2012): e1001298.

    Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage.
    Knoblich JA
    Nature 454.7201 (2008 Jul 10): 241-5.

    Poly(ADP-ribose) controls DE-cadherin-dependent stem cell maintenance and oocyte localization.
    Tulin AV
    Nature communications 3. (2012 Mar 27): 760.

    H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila.
    Kobayashi S
    Biology open 4.2 (2015 Jan 8): 119-24.

    The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway.
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Bicaudal-C recruits CCR4-NOT deadenylase to target mRNAs and regulates oogenesis, cytoskeletal organization, and its own expression.
    Lasko P
    Developmental cell 13.5 (2007 Nov): 691-704.

    Kinase-activity-independent functions of atypical protein kinase C in Drosophila.
    Wodarz A
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3759-71.

    Windei, the Drosophila homolog of mAM/MCAF1, is an essential cofactor of the H3K9 methyl transferase dSETDB1/Eggless in germ line development.
    Wodarz A
    PLoS genetics 5.9 (2009 Sep): e1000644.

    Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis.
    Lasko P
    PloS one 10.4 (2015): e0122519.

    <i>Drosophila</i> Mon1 constitutes a novel node in the brain-gonad axis that is essential for female germline maturation.
    Ratnaparkhi A
    Development (Cambridge, England) 146.13 (2019 Jul 10): .

    RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.
    Olenina LV
    European journal of cell biology 95.9 (2016 Sep): 311-22.

    Rasputin functions as a positive regulator of orb in Drosophila oogenesis.
    Schedl P
    PloS one 8.9 (2013): e72864.

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