Antigen: orb protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schedl, P.
Isotype: MIgG1, lambda light chain
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Multiple isoforms; major bands have an apparent MW between 97 and 100 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant fusion protein of phage T7 gene Phi10 and Oo18 RNA-binding protein (aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7])
Alternate Gene Names: CG10868
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7], containing the rrm (RNA recognition motif) regions
Uniprot ID: Q9VCN7
Immunogen Sequence: Partial protein
Entrez Gene ID: 42752
Antibody Registry ID: AB_528418
Additional Information: orb is a RNA binding protein
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
orb 4H8 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product orb 4H8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References