orb 6H4

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$40.00
SKU: orb 6H4
View product citations for antibody orb 6H4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: orb protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schedl, P.
Isotype: MIgG2a, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple isoforms; major bands have an apparent MW between 97 and 100 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: orb
Immunogen: Recombinant fusion protein of phage T7 gene Phi10 and oo18 RNA-binding protein (aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7])
Alternate Gene Names: CG10868
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7], containing the rrm (RNA recognition motif) regions
Uniprot ID: Q9VCN7 
Immunogen Sequence: Partial protein
Entrez Gene ID: 42752 
Additional Characterization:  
Antibody Registry ID: AB_528419 
Additional Information: orb is a RNA binding protein
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
orb 6H4 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product orb 6H4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster.
    Schedl P
    PLoS genetics 8.11 (2012): e1003079.

    The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
    Schedl P
    Genes & development 8.5 (1994 Mar 1): 598-613.

    A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
    St Johnston D
    Nature 421.6921 (2003 Jan 23): 379-84.

    Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway.
    Hazelrigg T
    Development (Cambridge, England) 129.1 (2002 Jan): 197-209.

    The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.
    Jongens TA
    Developmental cell 8.3 (2005 Mar): 331-42.

    Cup blocks the precocious activation of the orb autoregulatory loop.
    Schedl P
    PloS one 6.12 (2011): e28261.

    Rasputin functions as a positive regulator of orb in Drosophila oogenesis.
    Schedl P
    PloS one 8.9 (2013): e72864.

    Rab11 maintains connections between germline stem cells and niche cells in the Drosophila ovary.
    Cohen RS
    Development (Cambridge, England) 134.19 (2007 Oct): 3413-8.

    The homeobox gene mirror links EGF signalling to embryonic dorso-ventral axis formation through notch activation.
    Ruohola-Baker H
    Nature genetics 24.4 (2000 Apr): 429-33.

    Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate.
    Lehmann R
    Nature cell biology 6.5 (2004 May): 427-35.

    The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila.
    McKim KS
    Current biology : CB 13.4 (2003 Feb 18): 276-85.

    Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila.
    González-Reyes A
    PLoS biology 10.4 (2012): e1001298.

    WB References
    IP References
    Epitope Map References
    All References

    The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster.
    Schedl P
    PLoS genetics 8.11 (2012): e1003079.

    The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
    Schedl P
    Genes & development 8.5 (1994 Mar 1): 598-613.

    A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
    St Johnston D
    Nature 421.6921 (2003 Jan 23): 379-84.

    Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway.
    Hazelrigg T
    Development (Cambridge, England) 129.1 (2002 Jan): 197-209.

    The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.
    Jongens TA
    Developmental cell 8.3 (2005 Mar): 331-42.

    Cup blocks the precocious activation of the orb autoregulatory loop.
    Schedl P
    PloS one 6.12 (2011): e28261.

    Rasputin functions as a positive regulator of orb in Drosophila oogenesis.
    Schedl P
    PloS one 8.9 (2013): e72864.

    Rab11 maintains connections between germline stem cells and niche cells in the Drosophila ovary.
    Cohen RS
    Development (Cambridge, England) 134.19 (2007 Oct): 3413-8.

    The homeobox gene mirror links EGF signalling to embryonic dorso-ventral axis formation through notch activation.
    Ruohola-Baker H
    Nature genetics 24.4 (2000 Apr): 429-33.

    Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate.
    Lehmann R
    Nature cell biology 6.5 (2004 May): 427-35.

    The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila.
    McKim KS
    Current biology : CB 13.4 (2003 Feb 18): 276-85.

    Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila.
    González-Reyes A
    PLoS biology 10.4 (2012): e1001298.

    PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila.
    Simonelig M
    Development (Cambridge, England) 135.11 (2008 Jun): 1969-79.

    An essential cytoplasmic function for the nuclear poly(A) binding protein, PABP2, in poly(A) tail length control and early development in Drosophila.
    Simonelig M
    Developmental cell 9.4 (2005 Oct): 511-22.

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