10C9 anti-late bloomer

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$40.00
SKU: 10C9 anti-late bloomer
View product citations for antibody 10C9 anti-late bloomer on CiteAb

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Available: 70

DSHB Data Sheet

Catalog Fields

Antigen: Late Bloomer Protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California - Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody binds to non-permeabilized cells and is specific to motoneurons and some pheripheral neurons.
Antigen Molecular Weight: 23 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: lbm
Immunogen: Recombinant late bloomer extracellular domain between TM2 and TM3
Alternate Gene Names: CG2374
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q24188 
Immunogen Sequence:
Entrez Gene ID: 35623 
Additional Characterization:  
Antibody Registry ID: AB_528344 
Additional Information: FBgn0016032. RRID: AB_528344
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10C9 anti-late bloomer was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 10C9 anti-late bloomer)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    A neural tetraspanin, encoded by late bloomer, that facilitates synapse formation.
    Goodman CS
    Science (New York, N.Y.) 271.5257 (1996 Mar 29): 1867-70.

    IF References

    Nerfin-1 is required for early axon guidance decisions in the developing Drosophila CNS.
    Odenwald WF
    Developmental biology 277.2 (2005 Jan 15): 347-65.

    WB References

    Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons.
    Budnik V
    The Journal of biological chemistry 287.20 (2012 May 11): 16820-34.

    IHC References

    A neural tetraspanin, encoded by late bloomer, that facilitates synapse formation.
    Goodman CS
    Science (New York, N.Y.) 271.5257 (1996 Mar 29): 1867-70.

    All References

    A neural tetraspanin, encoded by late bloomer, that facilitates synapse formation.
    Goodman CS
    Science (New York, N.Y.) 271.5257 (1996 Mar 29): 1867-70.

    Nerfin-1 is required for early axon guidance decisions in the developing Drosophila CNS.
    Odenwald WF
    Developmental biology 277.2 (2005 Jan 15): 347-65.

    Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons.
    Budnik V
    The Journal of biological chemistry 287.20 (2012 May 11): 16820-34.

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