Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Nusse, R.
Isotype: MIgG2a, lambda light chain
Host Species: mouse
Depositors Institution: Department of Developmental Biology/HHMI
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: a fusion protein expressed in E. coli
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Designation of SP2/0
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_528257
Additional Information: The origin of the antibody is the cytoplasmic tail of fz2; the last 89 amino acids were fused to GST. Immunization used 30 µg/boost in 1997/1998. RRID:AB_528257
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12A7 was deposited to the DSHB by Nusse, R. (DSHB Hybridoma Product 12A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).