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SKU: 12A7
View product citations for antibody 12A7 on CiteAb

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Available: 149

DSHB Data Sheet

Catalog Fields

Antigen: Frizzled2
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Nusse, R.
Isotype: MIgG2a, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Developmental Biology/HHMI
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: a fusion protein expressed in E. coli
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of SP2/0
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528257 
Additional Information: The origin of the antibody is the cytoplasmic tail of fz2; the last 89 amino acids were fused to GST. Immunization used 30 µg/boost in 1997/1998. RRID:AB_528257
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12A7 was deposited to the DSHB by Nusse, R. (DSHB Hybridoma Product 12A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Development (Cambridge, England)
    Wieschaus E
    Development (Cambridge, England) 126.3 (1999 Feb): 577-86.

    All References

    Development (Cambridge, England)
    Wieschaus E
    Development (Cambridge, England) 126.3 (1999 Feb): 577-86.

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