Antigen: Frizzled protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Adler, P.N.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Biology Department, University of Virginia
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Immunohistochemistry is positive under correct conditions.
Antigen Molecular Weight: 62 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: A fusion protein made in E. coli was used as the immunogen.
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Sp 2/0 - Ag 14
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_528256
Additional Information: The origin of the immunogen is the first 252 amino acids of Fz were linked to beta-galactosidase. RRID:AB_528256
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1C11 was deposited to the DSHB by Adler, P.N. (DSHB Hybridoma Product 1C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).