15H2 anti-Robo3 cytoplasmic

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SKU: 15H2 anti-Robo3 cytoplasmic
View product citations for antibody 15H2 anti-Robo3 cytoplasmic on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 15H2 anti-Robo3 cytoplasmic
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_531800 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Drosophila protein made in E. coli
Immunogen Sequence:
Myeloma Strain: Designation of P3
Epitope Mapped: No
Antigen Name: Robo3 cytoplasmic (Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/1/2001
Antigen Molecular Weight:
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley, Department of Molecular and Cell Biology
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Immunization was administered through intraperitoneal and had an adjuvant Ribi.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications:  
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15H2 anti-Robo3 cytoplasmic was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 15H2 anti-Robo3 cytoplasmic)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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