C555.6D

(0)No Reviews yet
$50.00 to $600.00
SKU: C555.6D

In Stock

Available: 64

DSHB Data Sheet

Catalog Fields

Product Name/ID: C555.6D
Available to For-Profits: Yes
Alternate Antibody Name: anti-slit, anti-Slit-C
Gene Name: sli
Ab Isotype: MIgG2b, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528470 
Uniprot ID: P24014 
RRID:  
Entrez Gene ID: 36746 
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing the C-terminal region of slit, aa 1311-1480 (isoformorm A; Uniprot ID P24014-2)
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: Ag8
Epitope Mapped: Yes
Antigen Name: slit protein, C-terminal
Epitope Location or Sequence: C-terminal portion, aa 1311-1480 [see depositor notes]
Alternate Antigen Name:
Deposit Date: 5/2/2000
Antigen Molecular Weight: Predicted; 169 kDa; Apparent 190 kDa and smaller proteolytic fragments containing the C-terminal region of the Slit protein
Depositor: Artavanis-Tsakonas, S.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Drosophila
Depositor Notes: This antibody was generated against the C-terminal region of the protein slit
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C555.6D was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C555.6D)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes & development 4.12A (1990 Dec): 2169-87.

    Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos.
    Wood W
    Development (Cambridge, England) 137.10 (2010 May): 1625-33.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.
    Yang X
    Developmental biology 325.1 (2009 Jan 1): 15-23.

    Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
    Carmena A
    Development (Cambridge, England) 138.8 (2011 Apr): 1563-71.

    ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin.
    Hiromi Y
    Nature neuroscience 9.1 (2006 Jan): 58-66.

    The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion.
    Lee S
    Development (Cambridge, England) 134.9 (2007 May): 1767-77.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    Slit and Robo control the development of dendrites in Drosophila CNS.
    Chiba A
    Development (Cambridge, England) 134.21 (2007 Nov): 3795-804.

    WB References
    IHC References
    IP References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes & development 4.12A (1990 Dec): 2169-87.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    All References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes & development 4.12A (1990 Dec): 2169-87.

    Regulation of axon guidance by slit and netrin signaling in the Drosophila ventral nerve cord.
    Krishnan S
    Genetics 176.4 (2007 Aug): 2235-46.

    Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos.
    Wood W
    Development (Cambridge, England) 137.10 (2010 May): 1625-33.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.
    Yang X
    Developmental biology 325.1 (2009 Jan 1): 15-23.

    Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
    Carmena A
    Development (Cambridge, England) 138.8 (2011 Apr): 1563-71.

    ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin.
    Hiromi Y
    Nature neuroscience 9.1 (2006 Jan): 58-66.

    The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion.
    Lee S
    Development (Cambridge, England) 134.9 (2007 May): 1767-77.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    Slit and Robo control the development of dendrites in Drosophila CNS.
    Chiba A
    Development (Cambridge, England) 134.21 (2007 Nov): 3795-804.

    Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.
    Bashaw GJ
    PLoS genetics 11.9 (2015 Sep): e1005402.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review