C555.6D

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$40.00
SKU: C555.6D
View product citations for antibody C555.6D on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: slit protein, C-terminal
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Artavanis-Tsakonas, S.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody was generated against the C-terminal region of the protein slit
Antigen Molecular Weight: Predicted; 169 kDa; Apparent 190 kDa and smaller proteolytic fragments containing the C-terminal region of the Slit protein
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: sli
Immunogen: Recombinant fusion protein containing the C-terminal region of slit, aa 1311-1480 (isoformorm A; Uniprot ID P24014-2)
Alternate Gene Names: CG43758
Alternate Antibody Name: anti-slit, anti-Slit-C
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8
Epitope Location or Sequence: C-terminal portion, aa 1311-1480 [see depositor notes]
Uniprot ID: P24014 
Immunogen Sequence: Partial protein
Entrez Gene ID: 36746 
Additional Characterization:
Antibody Registry ID: AB_528470 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C555.6D was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C555.6D)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes and development 4.12A (1990 Dec): 2169-87.

    Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos.
    Wood W
    Development (Cambridge, England) 137.10 (2010 May): 1625-33.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.
    Yang X
    Developmental biology 325.1 (2009 Jan 1): 15-23.

    Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
    Carmena A
    Development (Cambridge, England) 138.8 (2011 Apr): 1563-71.

    ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin.
    Hiromi Y
    Nature neuroscience 9.1 (2006 Jan): 58-66.

    The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion.
    Lee S
    Development (Cambridge, England) 134.9 (2007 May): 1767-77.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    Slit and Robo control the development of dendrites in Drosophila CNS.
    Chiba A
    Development (Cambridge, England) 134.21 (2007 Nov): 3795-804.

    WB References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes and development 4.12A (1990 Dec): 2169-87.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    Slit and Robo control the development of dendrites in Drosophila CNS.
    Chiba A
    Development (Cambridge, England) 134.21 (2007 Nov): 3795-804.

    Regulation of axon guidance by slit and netrin signaling in the Drosophila ventral nerve cord.
    Krishnan S
    Genetics 176.4 (2007 Aug): 2235-46.

    Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.
    Bashaw GJ
    PLoS genetics 11.9 (2015 Sep): e1005402.

    IHC References
    IP References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes and development 4.12A (1990 Dec): 2169-87.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    All References

    slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
    Artavanis-Tsakonas S
    Genes and development 4.12A (1990 Dec): 2169-87.

    Regulation of axon guidance by slit and netrin signaling in the Drosophila ventral nerve cord.
    Krishnan S
    Genetics 176.4 (2007 Aug): 2235-46.

    Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos.
    Wood W
    Development (Cambridge, England) 137.10 (2010 May): 1625-33.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis.
    Yang X
    Developmental biology 325.1 (2009 Jan 1): 15-23.

    Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
    Carmena A
    Development (Cambridge, England) 138.8 (2011 Apr): 1563-71.

    ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin.
    Hiromi Y
    Nature neuroscience 9.1 (2006 Jan): 58-66.

    The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion.
    Lee S
    Development (Cambridge, England) 134.9 (2007 May): 1767-77.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    Slit and Robo control the development of dendrites in Drosophila CNS.
    Chiba A
    Development (Cambridge, England) 134.21 (2007 Nov): 3795-804.

    Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.
    Bashaw GJ
    PLoS genetics 11.9 (2015 Sep): e1005402.

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