C555.6D
Catalog Fields
Antigen: slit protein, C-terminal
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b, kappa light chain
Depositor: Artavanis-Tsakonas, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Harvard Medical School
Antigen Molecular Weight: Predicted; 169 kDa; Apparent 190 kDa and smaller proteolytic fragments containing the C-terminal region of the Slit protein
Depositors Notes: This antibody was generated against the C-terminal region of the protein slit
Immunogen: Recombinant fusion protein containing the C-terminal region of slit, aa 1311-1480 (isoformorm A; Uniprot ID P24014-2)
Gene: sli
Alternate Antibody Name: anti-slit, anti-Slit-C
Alternate Gene Names: CG43758
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Ag8
Epitope Mapped: Yes
Uniprot ID: P24014
Epitope Location or Sequence: C-terminal portion, aa 1311-1480 [see depositor notes]
Entrez Gene ID: 36746
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528470
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C555.6D was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C555.6D)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains.
Artavanis-Tsakonas S
Genes & development 4.12A (1990 Dec): 2169-87.