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14C9 anti-Robo3 extracellular

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SKU: 14C9 anti-Robo3 extracellular
View product citations for antibody 14C9 anti-Robo3 extracellular on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Robo3 extracellular (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Depositors Institution: University of California, Berkeley, Department of Molecular and Cell Biology
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Drosophila
Epitope Mapped: No
Immunogen: Drosophila protein made in E. coli
Additional Information: During immunization a route of intraperitoneal was used and the adjuvant was RIBI. RRID:AB_528454
Myeloma Strain: Designation of P3
Antibody Registry ID: AB_528454 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14C9 anti-Robo3 extracellular was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 14C9 anti-Robo3 extracellular)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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