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14C9 anti-Robo3 extracellular

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$40.00
SKU: 14C9 anti-Robo3 extracellular
View product citations for antibody 14C9 anti-Robo3 extracellular on CiteAb

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Available: 145

DSHB Data Sheet

Catalog Fields

Antigen: Robo3 extracellular (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley, Department of Molecular and Cell Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Drosophila protein made in E. coli
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation of P3
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528454 
Additional Information: During immunization a route of intraperitoneal was used and the adjuvant was RIBI. RRID:AB_528454
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14C9 anti-Robo3 extracellular was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 14C9 anti-Robo3 extracellular)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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