13C9 anti-Robo

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$40.00
SKU: 13C9 anti-Robo
View product citations for antibody 13C9 anti-Robo on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Robo (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence: AAF46887.1
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not cross-react with Robo2 or Robo3; Use fresh, not MeOH stored, embryo; Works well with with Tween, not Triton.
Antigen Molecular Weight: Predicted: 151.7 kDa; Apparent: 180kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: robo
Immunogen: extracellular portion (amino acids 404_725) of Robo turn from the commissure (Figure 5B) into a longitudinal
Alternate Gene Names: BEST:GM07780; CG13521; CT32892; D-Robo1; Dmel\CG13521; dRobo; dRobo1; Robo; ROBO; Robo-1; robo1; Robo1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3
Epitope Location or Sequence: Extracellular domain aa. 404_725
Uniprot ID: Q7KVK3 
Immunogen Sequence: 9458045
Entrez Gene ID: 37603 
Additional Characterization:
Antibody Registry ID: AB_2181861 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
13C9 anti-Robo was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 13C9 anti-Robo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The axon-guidance roundabout gene alters the pace of the Drosophila circadian clock.
    Ceriani MF
    The European journal of neuroscience 27.2 (2008 Jan): 396-407.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    The actin-binding protein Canoe/AF-6 forms a complex with Robo and is required for Slit-Robo signaling during axon pathfinding at the CNS midline.
    Carmena A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.29 (2012 Jul 18): 10035-44.

    A genetic screen for mutations affecting gonad formation in Drosophila reveals a role for the slit/robo pathway.
    Van Doren M
    Developmental biology 353.2 (2011 May 15): 217-28.

    Roundabout is required in the visceral mesoderm for proper microvillus length in the hindgut epithelium.
    Kramer SG
    Developmental dynamics : an official publication of the American Association of Anatomists 241.4 (2012 Apr): 759-69.

    WB References

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    The actin-binding protein Canoe/AF-6 forms a complex with Robo and is required for Slit-Robo signaling during axon pathfinding at the CNS midline.
    Carmena A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.29 (2012 Jul 18): 10035-44.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    IHC References

    NETRIN and SLIT guide salivary gland migration.
    Beckendorf SK
    Developmental biology 284.1 (2005 Aug 1): 102-11.

    Intra-axonal patterning: intrinsic compartmentalization of the axonal membrane in Drosophila neurons.
    Hiromi Y
    Neuron 64.2 (2009 Oct 29): 188-99.

    Roundabout 2 regulates migration of sensory neurons by signaling in trans.
    Zinn K
    Current biology : CB 14.15 (2004 Aug 10): 1319-29.

    The actin-binding protein Canoe/AF-6 forms a complex with Robo and is required for Slit-Robo signaling during axon pathfinding at the CNS midline.
    Carmena A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.29 (2012 Jul 18): 10035-44.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    Heparan sulfate proteoglycan specificity during axon pathway formation in the Drosophila embryo.
    Johnson KG
    Developmental neurobiology 71.7 (2011 Jul): 608-18.

    IP References

    The actin-binding protein Canoe/AF-6 forms a complex with Robo and is required for Slit-Robo signaling during axon pathfinding at the CNS midline.
    Carmena A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.29 (2012 Jul 18): 10035-44.

    Epitope Map References
    All References

    NETRIN and SLIT guide salivary gland migration.
    Beckendorf SK
    Developmental biology 284.1 (2005 Aug 1): 102-11.

    Intra-axonal patterning: intrinsic compartmentalization of the axonal membrane in Drosophila neurons.
    Hiromi Y
    Neuron 64.2 (2009 Oct 29): 188-99.

    Roundabout 2 regulates migration of sensory neurons by signaling in trans.
    Zinn K
    Current biology : CB 14.15 (2004 Aug 10): 1319-29.

    The actin-binding protein Canoe/AF-6 forms a complex with Robo and is required for Slit-Robo signaling during axon pathfinding at the CNS midline.
    Carmena A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.29 (2012 Jul 18): 10035-44.

    Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.
    Garrity PA
    Development (Cambridge, England) 131.23 (2004 Dec): 5935-45.

    Heparan sulfate proteoglycan specificity during axon pathway formation in the Drosophila embryo.
    Johnson KG
    Developmental neurobiology 71.7 (2011 Jul): 608-18.

    The axon-guidance roundabout gene alters the pace of the Drosophila circadian clock.
    Ceriani MF
    The European journal of neuroscience 27.2 (2008 Jan): 396-407.

    Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance.
    Bhat MA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.16 (2010 Apr 21): 5653-67.

    A genetic screen for mutations affecting gonad formation in Drosophila reveals a role for the slit/robo pathway.
    Van Doren M
    Developmental biology 353.2 (2011 May 15): 217-28.

    Roundabout is required in the visceral mesoderm for proper microvillus length in the hindgut epithelium.
    Kramer SG
    Developmental dynamics : an official publication of the American Association of Anatomists 241.4 (2012 Apr): 759-69.

    Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors.
    Tear G
    Cell 92.2 (1998 Jan 23): 205-15.

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