bam
Catalog Fields
Antigen: Fly Bag-of-marbles
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: McKearin, D.
Isotype: MIgG1
Depositors Institution: Howard Hughes Medical Institute
Host Species: mouse
Depositors Notes: Weakly positive in western blots.
Positive Tested Species Reactivity: Drosophila
Gene: bam
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 55kDa
Alternate Gene Names: CG10422, BamC
Immunogen: Recombinant Bag-of-marbles protein fragment fused to His-tag
Clonality: Monoclonal
Alternate Antibody Name: anti-BamC
Immunogen Sequence: Partial protein
Uniprot ID: P22745
Entrez Gene ID: 43038
Antibody Registry ID: AB_10570327
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bam was deposited to the DSHB by McKearin, D. (DSHB Hybridoma Product bam)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.
Maines JZ
Proceedings of the National Academy of Sciences of the United States of America 106.23 (2009 Jun 9): 9304-9.