bam

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SKU: bam
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DSHB Data Sheet

Catalog Fields

Antigen: Fly Bag-of-marbles
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: McKearin, D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Howard Hughes Medical Institute
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Weakly positive in western blots.
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 55kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: bam
Immunogen: Recombinant Bag-of-marbles protein fragment fused to His-tag
Alternate Gene Names: CG10422, BamC
Alternate Antibody Name: anti-BamC
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P22745 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43038 
Additional Characterization:  
Antibody Registry ID: AB_10570327 
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bam was deposited to the DSHB by McKearin, D. (DSHB Hybridoma Product bam)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.
    Maines JZ
    Proceedings of the National Academy of Sciences of the United States of America 106.23 (2009 Jun 9): 9304-9.

    IF References

    Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.
    Maines JZ
    Proceedings of the National Academy of Sciences of the United States of America 106.23 (2009 Jun 9): 9304-9.

    Somatic cell lineage is required for differentiation and not maintenance of germline stem cells in Drosophila testes.
    Fuller MT
    Proceedings of the National Academy of Sciences of the United States of America 109.45 (2012 Nov 6): 18477-81.

    Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling.
    Lai EC
    Current biology : CB 23.15 (2013 Aug 5): 1442-8.

    Dmp53 is sequestered to nuclear bodies in spermatogonia of Drosophila melanogaster.
    Hime GR
    Cell and tissue research 350.2 (2012 Nov): 385-94.

    dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary.
    Li M
    Developmental biology 379.2 (2013 Jul 15): 167-81.

    The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    Li M
    Developmental biology 384.1 (2013 Dec 1): 1-12.

    Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling.
    Buszczak M
    Development (Cambridge, England) 139.9 (2012 May): 1547-56.

    Mei-p26 cooperates with Bam, Bgcn and Sxl to promote early germline development in the Drosophila ovary.
    Buszczak M
    PloS one 8.3 (2013): e58301.

    Germ line differentiation factor Bag of Marbles is a regulator of hematopoietic progenitor maintenance during Drosophila hematopoiesis.
    Schulz RA
    Development (Cambridge, England) 138.18 (2011 Sep): 3879-84.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    MicroRNAs downregulate Bag of marbles to ensure proper terminal differentiation in the Drosophila male germline.
    Chen X
    Development (Cambridge, England) 140.1 (2013 Jan 1): 23-30.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    Self-limiting stem-cell niche signaling through degradation of a stem-cell receptor.
    Inaba M
    PLoS biology 18.12 (2020 Dec): e3001003.

    All References

    Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.
    Maines JZ
    Proceedings of the National Academy of Sciences of the United States of America 106.23 (2009 Jun 9): 9304-9.

    Somatic cell lineage is required for differentiation and not maintenance of germline stem cells in Drosophila testes.
    Fuller MT
    Proceedings of the National Academy of Sciences of the United States of America 109.45 (2012 Nov 6): 18477-81.

    Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling.
    Lai EC
    Current biology : CB 23.15 (2013 Aug 5): 1442-8.

    Dmp53 is sequestered to nuclear bodies in spermatogonia of Drosophila melanogaster.
    Hime GR
    Cell and tissue research 350.2 (2012 Nov): 385-94.

    dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary.
    Li M
    Developmental biology 379.2 (2013 Jul 15): 167-81.

    The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    Li M
    Developmental biology 384.1 (2013 Dec 1): 1-12.

    Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling.
    Buszczak M
    Development (Cambridge, England) 139.9 (2012 May): 1547-56.

    Mei-p26 cooperates with Bam, Bgcn and Sxl to promote early germline development in the Drosophila ovary.
    Buszczak M
    PloS one 8.3 (2013): e58301.

    Germ line differentiation factor Bag of Marbles is a regulator of hematopoietic progenitor maintenance during Drosophila hematopoiesis.
    Schulz RA
    Development (Cambridge, England) 138.18 (2011 Sep): 3879-84.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    MicroRNAs downregulate Bag of marbles to ensure proper terminal differentiation in the Drosophila male germline.
    Chen X
    Development (Cambridge, England) 140.1 (2013 Jan 1): 23-30.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    Self-limiting stem-cell niche signaling through degradation of a stem-cell receptor.
    Inaba M
    PLoS biology 18.12 (2020 Dec): e3001003.

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