bam
Catalog Fields
Antigen: Fly Bag-of-marbles
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: McKearin, D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Howard Hughes Medical Institute
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Weakly positive in western blots.
Predicted Species Reactivity:
Gene: bam
Immunogen: Recombinant Bag-of-marbles protein fragment fused to His-tag
Alternate Gene Names: CG10422, BamC
Alternate Antibody Name: anti-BamC
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P22745
Immunogen Sequence: Partial protein
Entrez Gene ID: 43038
Antibody Registry ID: AB_10570327
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bam was deposited to the DSHB by McKearin, D. (DSHB Hybridoma Product bam)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.
Maines JZ
Proceedings of the National Academy of Sciences of the United States of America 106.23 (2009 Jun 9): 9304-9.