bam
Catalog Fields
Clone ID/Product Name: bam
Available to For-Profits: Yes
Alternate Antibody Name: anti-BamC
Gene Symbol: bam
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_10570327
Uniprot ID: P22745
Entrez Gene ID: 43038
Clonality: Monoclonal
Immunogen: Recombinant Bag-of-marbles protein fragment fused to His-tag
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Fly Bag-of-marbles
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/31/2009
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 55kDa
Depositor: McKearin, D.
Antigen Sequence:
Depositor Institution: Howard Hughes Medical Institute
Antigen Species: Drosophila
Depositor Notes: Weakly positive in western blots.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
bam was deposited to the DSHB by McKearin, D. (DSHB Hybridoma Product bam)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.
Maines JZ
Proceedings of the National Academy of Sciences of the United States of America 106.23 (2009 Jun 9): 9304-9.