2B8

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$40.00
SKU: 2B8
View product citations for antibody 2B8 on CiteAb

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Available: 48

DSHB Data Sheet

Catalog Fields

Antigen: Even-skipped protein
Hybridoma Cells Available: Yes
Antigen Species: Grasshopper
Depositor: Zinn, K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila, Insect
Depositors Notes: Published reports indicate this antibody recognizes specific protein in drosophila, grasshopper, locusts, crickets, beetles, mosquitos fly,springtail and honey bees.
Antigen Molecular Weight: Predicted: 29KDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Eve
Immunogen: C-terminal 2/3 of even-skipped.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: HL-1
Epitope Location or Sequence:
Uniprot ID: Q26472 
Immunogen Sequence: Starting Nucleotide 304 of cDNA
Entrez Gene ID: 36039 
Additional Characterization:
Antibody Registry ID: AB_528230 
Additional Information:
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B8 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 2B8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Maternal and zygotic requirements for src64 during Drosophila cellularization.
    Thomas JH
    Genesis (New York, N.Y. : 2000) 49.12 (2011 Dec): 912-8.

    IHC References

    Pair-rule expression patterns of even-skipped are found in both short- and long-germ beetles.
    Zinn K
    Nature 367.6462 (1994 Feb 3): 429-34.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    even-skipped has gap-like, pair-rule-like, and segmental functions in the cricket Gryllus bimaculatus, a basal, intermediate germ insect (Orthoptera).
    Noji S
    Developmental biology 303.1 (2007 Mar 1): 202-13.

    The repressor activity of Even-skipped is highly conserved, and is sufficient to activate engrailed and to regulate both the spacing and stability of parasegment boundaries.
    Jaynes JB
    Development (Cambridge, England) 129.19 (2002 Oct): 4411-21.

    Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods.
    Patel NH
    Development (Cambridge, England) 126.11 (1999 Jun): 2327-34.

    Even-skipped, acting as a repressor, regulates axonal projections in Drosophila.
    Jaynes JB
    Development (Cambridge, England) 130.22 (2003 Nov): 5385-400.

    Caudal regulates the spatiotemporal dynamics of pair-rule waves in Tribolium.
    Brown SJ
    PLoS genetics 10.10 (2014 Oct): e1004677.

    even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone.
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    pygopus Encodes a nuclear protein essential for wingless/Wnt signaling.
    Lin X
    Development (Cambridge, England) 129.17 (2002 Sep): 4089-101.

    The Batten disease Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development.
    Denefrio C
    PloS one 5.12 (2010 Dec 22): e14402.

    Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein.
    Yang X
    Molecular and cellular biology 22.12 (2002 Jun): 4230-40.

    All References

    Pair-rule expression patterns of even-skipped are found in both short- and long-germ beetles.
    Zinn K
    Nature 367.6462 (1994 Feb 3): 429-34.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    even-skipped has gap-like, pair-rule-like, and segmental functions in the cricket Gryllus bimaculatus, a basal, intermediate germ insect (Orthoptera).
    Noji S
    Developmental biology 303.1 (2007 Mar 1): 202-13.

    The repressor activity of Even-skipped is highly conserved, and is sufficient to activate engrailed and to regulate both the spacing and stability of parasegment boundaries.
    Jaynes JB
    Development (Cambridge, England) 129.19 (2002 Oct): 4411-21.

    Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods.
    Patel NH
    Development (Cambridge, England) 126.11 (1999 Jun): 2327-34.

    Even-skipped, acting as a repressor, regulates axonal projections in Drosophila.
    Jaynes JB
    Development (Cambridge, England) 130.22 (2003 Nov): 5385-400.

    Caudal regulates the spatiotemporal dynamics of pair-rule waves in Tribolium.
    Brown SJ
    PLoS genetics 10.10 (2014 Oct): e1004677.

    even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone.
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    pygopus Encodes a nuclear protein essential for wingless/Wnt signaling.
    Lin X
    Development (Cambridge, England) 129.17 (2002 Sep): 4089-101.

    The Batten disease Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development.
    Denefrio C
    PloS one 5.12 (2010 Dec 22): e14402.

    Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein.
    Yang X
    Molecular and cellular biology 22.12 (2002 Jun): 4230-40.

    Maternal and zygotic requirements for src64 during Drosophila cellularization.
    Thomas JH
    Genesis (New York, N.Y. : 2000) 49.12 (2011 Dec): 912-8.

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