2B8
Catalog Fields
Antigen: Even-skipped protein
Available to For-Profits: Yes
Antigen Species: Grasshopper
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Zinn, K.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Beetle, Drosophila, Insect
Depositors Institution: California Institute of Technology
Antigen Molecular Weight: Predicted: 29KDa
Depositors Notes: Published reports indicate this antibody recognizes specific protein in drosophila, grasshopper, locusts, crickets, beetles, mosquitos fly,springtail and honey bees.
Immunogen: C-terminal 2/3 of even-skipped.
Gene: Eve
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: HL-1
Epitope Mapped: No
Uniprot ID: Q26472
Epitope Location or Sequence:
Entrez Gene ID: 36039
Immunogen Sequence: Starting Nucleotide 304 of cDNA
Antibody Registry ID: AB_528230
Recommended Applications: Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B8 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 2B8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Pair-rule expression patterns of even-skipped are found in both short- and long-germ beetles.
Zinn K
Nature 367.6462 (1994 Feb 3): 429-34.