2B8

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SKU: 2B8

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 2B8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Eve
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528230 
Uniprot ID: Q26472 
RRID:  
Entrez Gene ID: 36039 
Clonality: Monoclonal
Immunogen: C-terminal 2/3 of even-skipped.
Clone:
Immunogen Sequence: Starting Nucleotide 304 of cDNA
Myeloma Strain: HL-1
Epitope Mapped: No
Antigen Name: Even-skipped protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/2/1998
Antigen Molecular Weight: Predicted: 29KDa
Depositor: Zinn, K.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Grasshopper
Depositor Notes: Published reports indicate this antibody recognizes specific protein in drosophila, grasshopper, locusts, crickets, beetles, mosquitos fly,springtail and honey bees.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Beetle, Drosophila, Insect
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B8 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 2B8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

15 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References

    Pair-rule expression patterns of even-skipped are found in both short- and long-germ beetles.
    Zinn K
    Nature 367.6462 (1994 Feb 3): 429-34.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    even-skipped has gap-like, pair-rule-like, and segmental functions in the cricket Gryllus bimaculatus, a basal, intermediate germ insect (Orthoptera).
    Noji S
    Developmental biology 303.1 (2007 Mar 1): 202-13.

    The repressor activity of Even-skipped is highly conserved, and is sufficient to activate engrailed and to regulate both the spacing and stability of parasegment boundaries.
    Jaynes JB
    Development (Cambridge, England) 129.19 (2002 Oct): 4411-21.

    Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods.
    Patel NH
    Development (Cambridge, England) 126.11 (1999 Jun): 2327-34.

    Even-skipped, acting as a repressor, regulates axonal projections in Drosophila.
    Jaynes JB
    Development (Cambridge, England) 130.22 (2003 Nov): 5385-400.

    Caudal regulates the spatiotemporal dynamics of pair-rule waves in Tribolium.
    Brown SJ
    PLoS genetics 10.10 (2014 Oct): e1004677.

    even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone.
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    pygopus Encodes a nuclear protein essential for wingless/Wnt signaling.
    Lin X
    Development (Cambridge, England) 129.17 (2002 Sep): 4089-101.

    The Batten disease Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development.
    Denefrio C
    PloS one 5.12 (2010 Dec 22): e14402.

    Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein.
    Yang X
    Molecular and cellular biology 22.12 (2002 Jun): 4230-40.

    A pair-rule function of odd-skipped in germband stages of Tribolium development.
    Choe CP
    Developmental biology 465.1 (2020 Sep 1): 58-65.

    All References

    Pair-rule expression patterns of even-skipped are found in both short- and long-germ beetles.
    Zinn K
    Nature 367.6462 (1994 Feb 3): 429-34.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    even-skipped has gap-like, pair-rule-like, and segmental functions in the cricket Gryllus bimaculatus, a basal, intermediate germ insect (Orthoptera).
    Noji S
    Developmental biology 303.1 (2007 Mar 1): 202-13.

    The repressor activity of Even-skipped is highly conserved, and is sufficient to activate engrailed and to regulate both the spacing and stability of parasegment boundaries.
    Jaynes JB
    Development (Cambridge, England) 129.19 (2002 Oct): 4411-21.

    Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods.
    Patel NH
    Development (Cambridge, England) 126.11 (1999 Jun): 2327-34.

    Even-skipped, acting as a repressor, regulates axonal projections in Drosophila.
    Jaynes JB
    Development (Cambridge, England) 130.22 (2003 Nov): 5385-400.

    Caudal regulates the spatiotemporal dynamics of pair-rule waves in Tribolium.
    Brown SJ
    PLoS genetics 10.10 (2014 Oct): e1004677.

    even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone.
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    pygopus Encodes a nuclear protein essential for wingless/Wnt signaling.
    Lin X
    Development (Cambridge, England) 129.17 (2002 Sep): 4089-101.

    The Batten disease Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development.
    Denefrio C
    PloS one 5.12 (2010 Dec 22): e14402.

    Membrane targeting and asymmetric localization of Drosophila partner of inscuteable are discrete steps controlled by distinct regions of the protein.
    Yang X
    Molecular and cellular biology 22.12 (2002 Jun): 4230-40.

    A pair-rule function of odd-skipped in germband stages of Tribolium development.
    Choe CP
    Developmental biology 465.1 (2020 Sep 1): 58-65.

    Maternal and zygotic requirements for src64 during Drosophila cellularization.
    Thomas JH
    Genesis (New York, N.Y. : 2000) 49.12 (2011 Dec): 912-8.

    A novel temporal identity window generates alternating Eve(+)/Nkx6(+) motor neuron subtypes in a single progenitor lineage.
    Doe CQ
    Neural development 15.1 (2020 Jul 28): 9.

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