3C10 anti-even skipped

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 3C10 anti-even skipped
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Eve
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528229 
Uniprot ID: P06602 
RRID:  
Entrez Gene ID: 36039 
Clonality: Monoclonal
Immunogen: Even-skipped protein expressed in E. coli.
Clone:
Immunogen Sequence: Recombinant even-skipped protein
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Even-skipped protein (Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight: Predicted: 40kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: This antibody is useful for following the development of a small set of neurons including qCC, pCC, RP2, and CQ and EL clusters [see PMID: 501154].
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila, Grasshopper
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3C10 anti-even skipped was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 3C10 anti-even skipped)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
    Carthew RW
    Developmental biology 385.2 (2014 Jan 15): 263-78.

    Temporal pattern of the posterior expression of Wingless in Drosophila blastoderm.
    De Robertis EM
    Gene expression patterns : GEP 11.7 (2011 Oct): 456-63.

    The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Genetics 193.2 (2013 Feb): 633-49.

    A conditional tissue-specific transgene expression system using inducible GAL4.
    Keshishian H
    Proceedings of the National Academy of Sciences of the United States of America 98.22 (2001 Oct 23): 12596-601.

    Developmentally regulated subnuclear genome reorganization restricts neural progenitor competence in Drosophila.
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    Segmental differences in firing properties and potassium currents in Drosophila larval motoneurons.
    Levine RB
    Journal of neurophysiology 107.5 (2012 Mar): 1356-65.

    The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity.
    Doe CQ
    Development (Cambridge, England) 138.9 (2011 May): 1727-35.

    Drosophila serpin 4 functions as a neuroserpin-like inhibitor of subtilisin-like proprotein convertases.
    Keshishian H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.24 (2004 Jun 16): 5482-91.

    Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons.
    Doe CQ
    Gene expression patterns : GEP 12.1-2 (2012 Jan-Feb): 11-7.

    TALE-mediated modulation of transcriptional enhancers in vivo.
    Stern DL
    Nature methods 10.8 (2013 Aug): 762-7.

    Coe genes are expressed in differentiating neurons in the central nervous system of protostomes.
    Vervoort M
    PloS one 6.6 (2011): e21213.

    PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system.
    Kjaerulff O
    The Journal of comparative neurology 517.3 (2009 Nov 20): 313-32.

    Sterile alpha motif domain-mediated self-association plays an essential role in modulating the activity of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Molecular and cellular biology 30.5 (2010 Mar): 1158-70.

    WB References
    IHC References
    All References

    Changing role of even-skipped during the evolution of insect pattern formation.
    Goodman CS
    Nature 357.6376 (1992 May 28): 339-42.

    Evidence for differential and redundant function of the Sox genes Dichaete and SoxN during CNS development in Drosophila.
    Russell S
    Development (Cambridge, England) 129.18 (2002 Sep): 4219-28.

    Frizzled and Dfrizzled-2 function as redundant receptors for Wingless during Drosophila embryonic development.
    Cadigan KM
    Development (Cambridge, England) 126.18 (1999 Sep): 4175-86.

    WASP and SCAR have distinct roles in activating the Arp2/3 complex during myoblast fusion.
    Onel SF
    Journal of cell science 121.Pt 8 (2008 Apr 15): 1303-13.

    Coe genes are expressed in differentiating neurons in the central nervous system of protostomes.
    Vervoort M
    PloS one 6.6 (2011): e21213.

    Tailless patterning functions are conserved in the honeybee even in the absence of Torso signaling.
    Dearden PK
    Developmental biology 335.1 (2009 Nov 1): 276-87.

    A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
    Carthew RW
    Developmental biology 385.2 (2014 Jan 15): 263-78.

    Temporal pattern of the posterior expression of Wingless in Drosophila blastoderm.
    De Robertis EM
    Gene expression patterns : GEP 11.7 (2011 Oct): 456-63.

    The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Genetics 193.2 (2013 Feb): 633-49.

    A conditional tissue-specific transgene expression system using inducible GAL4.
    Keshishian H
    Proceedings of the National Academy of Sciences of the United States of America 98.22 (2001 Oct 23): 12596-601.

    Developmentally regulated subnuclear genome reorganization restricts neural progenitor competence in Drosophila.
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    Segmental differences in firing properties and potassium currents in Drosophila larval motoneurons.
    Levine RB
    Journal of neurophysiology 107.5 (2012 Mar): 1356-65.

    The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity.
    Doe CQ
    Development (Cambridge, England) 138.9 (2011 May): 1727-35.

    Drosophila serpin 4 functions as a neuroserpin-like inhibitor of subtilisin-like proprotein convertases.
    Keshishian H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.24 (2004 Jun 16): 5482-91.

    Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons.
    Doe CQ
    Gene expression patterns : GEP 12.1-2 (2012 Jan-Feb): 11-7.

    TALE-mediated modulation of transcriptional enhancers in vivo.
    Stern DL
    Nature methods 10.8 (2013 Aug): 762-7.

    PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system.
    Kjaerulff O
    The Journal of comparative neurology 517.3 (2009 Nov 20): 313-32.

    Sterile alpha motif domain-mediated self-association plays an essential role in modulating the activity of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Molecular and cellular biology 30.5 (2010 Mar): 1158-70.

    even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone.
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    Primary human bone cultures from older patients do not respond at continuum levels of in vivo strain magnitudes.
    Brand RA
    Journal of biomechanics 33.1 (2000 Jan): 63-71.

    Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31.
    Sibon OC
    Developmental biology 313.2 (2008 Jan 15): 802-13.

    Regulation of central neuron synaptic targeting by the Drosophila POU protein, Acj6.
    Johnson WA
    Development (Cambridge, England) 127.11 (2000 Jun): 2395-405.

    Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development.
    Terman JR
    Development (Cambridge, England) 134.12 (2007 Jun): 2337-47.

    The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding.
    Wyman RJ
    Neural development 4. (2009 Aug 17): 31.

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