3C10 anti-even skipped

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$40.00
SKU: 3C10 anti-even skipped
View product citations for antibody 3C10 anti-even skipped on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Even-skipped protein (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila, Grasshopper
Depositors Notes: This antibody is useful for following the development of a small set of neurons including qCC, pCC, RP2, and CQ and EL clusters [see PMID: 501154].
Antigen Molecular Weight: Predicted: 40kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Eve
Immunogen: Even-skipped protein expressed in E. coli.
Alternate Gene Names: Eve; EVE; eve2; even;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P06602 
Immunogen Sequence: Recombinant even-skipped protein
Entrez Gene ID: 36039 
Additional Characterization:
Antibody Registry ID: AB_528229 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3C10 anti-even skipped was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 3C10 anti-even skipped)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
    Carthew RW
    Developmental biology 385.2 (2014 Jan 15): 263-78.

    Temporal pattern of the posterior expression of Wingless in Drosophila blastoderm.
    De Robertis EM
    Gene expression patterns : GEP 11.7 (2011 Oct): 456-63.

    The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Genetics 193.2 (2013 Feb): 633-49.

    A conditional tissue-specific transgene expression system using inducible GAL4.
    Keshishian H
    Proceedings of the National Academy of Sciences of the United States of America 98.22 (2001 Oct 23): 12596-601.

    Developmentally regulated subnuclear genome reorganization restricts neural progenitor competence in Drosophila.
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    Segmental differences in firing properties and potassium currents in Drosophila larval motoneurons.
    Levine RB
    Journal of neurophysiology 107.5 (2012 Mar): 1356-65.

    The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity.
    Doe CQ
    Development (Cambridge, England) 138.9 (2011 May): 1727-35.

    Drosophila serpin 4 functions as a neuroserpin-like inhibitor of subtilisin-like proprotein convertases.
    Keshishian H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.24 (2004 Jun 16): 5482-91.

    Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons.
    Doe CQ
    Gene expression patterns : GEP 12.1-2 (2012 Jan-Feb): 11-7.

    TALE-mediated modulation of transcriptional enhancers in vivo.
    Stern DL
    Nature methods 10.8 (2013 Aug): 762-7.

    Coe genes are expressed in differentiating neurons in the central nervous system of protostomes.
    Vervoort M
    PloS one 6.6 (2011): e21213.

    PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system.
    Kjaerulff O
    The Journal of comparative neurology 517.3 (2009 Nov 20): 313-32.

    Sterile alpha motif domain-mediated self-association plays an essential role in modulating the activity of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Molecular and cellular biology 30.5 (2010 Mar): 1158-70.

    WB References
    IHC References
    All References

    Changing role of even-skipped during the evolution of insect pattern formation.
    Goodman CS
    Nature 357.6376 (1992 May 28): 339-42.

    Evidence for differential and redundant function of the Sox genes Dichaete and SoxN during CNS development in Drosophila.
    Russell S
    Development (Cambridge, England) 129.18 (2002 Sep): 4219-28.

    Frizzled and Dfrizzled-2 function as redundant receptors for Wingless during Drosophila embryonic development.
    Cadigan KM
    Development (Cambridge, England) 126.18 (1999 Sep): 4175-86.

    WASP and SCAR have distinct roles in activating the Arp2/3 complex during myoblast fusion.
    Onel SF
    Journal of cell science 121.Pt 8 (2008 Apr 15): 1303-13.

    Coe genes are expressed in differentiating neurons in the central nervous system of protostomes.
    Vervoort M
    PloS one 6.6 (2011): e21213.

    Tailless patterning functions are conserved in the honeybee even in the absence of Torso signaling.
    Dearden PK
    Developmental biology 335.1 (2009 Nov 1): 276-87.

    A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
    Carthew RW
    Developmental biology 385.2 (2014 Jan 15): 263-78.

    Temporal pattern of the posterior expression of Wingless in Drosophila blastoderm.
    De Robertis EM
    Gene expression patterns : GEP 11.7 (2011 Oct): 456-63.

    The relationship between long-range chromatin occupancy and polymerization of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Genetics 193.2 (2013 Feb): 633-49.

    A conditional tissue-specific transgene expression system using inducible GAL4.
    Keshishian H
    Proceedings of the National Academy of Sciences of the United States of America 98.22 (2001 Oct 23): 12596-601.

    Developmentally regulated subnuclear genome reorganization restricts neural progenitor competence in Drosophila.
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    Segmental differences in firing properties and potassium currents in Drosophila larval motoneurons.
    Levine RB
    Journal of neurophysiology 107.5 (2012 Mar): 1356-65.

    The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity.
    Doe CQ
    Development (Cambridge, England) 138.9 (2011 May): 1727-35.

    Drosophila serpin 4 functions as a neuroserpin-like inhibitor of subtilisin-like proprotein convertases.
    Keshishian H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.24 (2004 Jun 16): 5482-91.

    Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons.
    Doe CQ
    Gene expression patterns : GEP 12.1-2 (2012 Jan-Feb): 11-7.

    TALE-mediated modulation of transcriptional enhancers in vivo.
    Stern DL
    Nature methods 10.8 (2013 Aug): 762-7.

    PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system.
    Kjaerulff O
    The Journal of comparative neurology 517.3 (2009 Nov 20): 313-32.

    Sterile alpha motif domain-mediated self-association plays an essential role in modulating the activity of the Drosophila ETS family transcriptional repressor Yan.
    Rebay I
    Molecular and cellular biology 30.5 (2010 Mar): 1158-70.

    even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone.
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    Primary human bone cultures from older patients do not respond at continuum levels of in vivo strain magnitudes.
    Brand RA
    Journal of biomechanics 33.1 (2000 Jan): 63-71.

    Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31.
    Sibon OC
    Developmental biology 313.2 (2008 Jan 15): 802-13.

    Regulation of central neuron synaptic targeting by the Drosophila POU protein, Acj6.
    Johnson WA
    Development (Cambridge, England) 127.11 (2000 Jun): 2395-405.

    Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development.
    Terman JR
    Development (Cambridge, England) 134.12 (2007 Jun): 2337-47.

    The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding.
    Wyman RJ
    Neural development 4. (2009 Aug 17): 31.

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