3C10 anti-even skipped

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$40.00
SKU: 3C10 anti-even skipped
View product citations for antibody 3C10 anti-even skipped on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Even-skipped protein (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila, Grasshopper
Depositors Notes: This antibody is useful for following the development of a small set of neurons including qCC, pCC, RP2, and CQ and EL clusters [see PMID: 501154].
Antigen Molecular Weight: Predicted: 40kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Eve
Immunogen: Even-skipped protein expressed in E. coli.
Alternate Gene Names: Eve; EVE; eve2; even;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P06602 
Immunogen Sequence: Recombinant even-skipped protein
Entrez Gene ID: 36039 
Additional Characterization:
Antibody Registry ID: AB_528229 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3C10 anti-even skipped was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 3C10 anti-even skipped)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Nature
    Goodman CS
    Nature 357.6376 (1992 May 28): 339-42.

    IF References

    Developmental biology
    Carthew RW
    Developmental biology 385.2 (2014 Jan 15): 263-78.

    Gene expression patterns : GEP
    De Robertis EM
    Gene expression patterns : GEP 11.7 (2011 Oct): 456-63.

    Genetics
    Rebay I
    Genetics 193.2 (2013 Feb): 633-49.

    Proceedings of the National Academy of Sciences of the United States of America
    Keshishian H
    Proceedings of the National Academy of Sciences of the United States of America 98.22 (2001 Oct 23): 12596-601.

    Cell
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    Journal of neurophysiology
    Levine RB
    Journal of neurophysiology 107.5 (2012 Mar): 1356-65.

    Development (Cambridge, England)
    Doe CQ
    Development (Cambridge, England) 138.9 (2011 May): 1727-35.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Keshishian H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.24 (2004 Jun 16): 5482-91.

    Gene expression patterns : GEP
    Doe CQ
    Gene expression patterns : GEP 12.1-2 (2012 Jan-Feb): 11-7.

    Nature methods
    Stern DL
    Nature methods 10.8 (2013 Aug): 762-7.

    PloS one
    Vervoort M
    PloS one 6.6 (2011): e21213.

    The Journal of comparative neurology
    Kjaerulff O
    The Journal of comparative neurology 517.3 (2009 Nov 20): 313-32.

    Molecular and cellular biology
    Rebay I
    Molecular and cellular biology 30.5 (2010 Mar): 1158-70.

    WB References

    Development (Cambridge, England)
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    IHC References

    Nature
    Goodman CS
    Nature 357.6376 (1992 May 28): 339-42.

    Development (Cambridge, England)
    Russell S
    Development (Cambridge, England) 129.18 (2002 Sep): 4219-28.

    Development (Cambridge, England)
    Cadigan KM
    Development (Cambridge, England) 126.18 (1999 Sep): 4175-86.

    Journal of cell science
    Onel SF
    Journal of cell science 121.Pt 8 (2008 Apr 15): 1303-13.

    PloS one
    Vervoort M
    PloS one 6.6 (2011): e21213.

    Developmental biology
    Dearden PK
    Developmental biology 335.1 (2009 Nov 1): 276-87.

    All References

    Nature
    Goodman CS
    Nature 357.6376 (1992 May 28): 339-42.

    Development (Cambridge, England)
    Russell S
    Development (Cambridge, England) 129.18 (2002 Sep): 4219-28.

    Development (Cambridge, England)
    Cadigan KM
    Development (Cambridge, England) 126.18 (1999 Sep): 4175-86.

    Journal of cell science
    Onel SF
    Journal of cell science 121.Pt 8 (2008 Apr 15): 1303-13.

    PloS one
    Vervoort M
    PloS one 6.6 (2011): e21213.

    Developmental biology
    Dearden PK
    Developmental biology 335.1 (2009 Nov 1): 276-87.

    Developmental biology
    Carthew RW
    Developmental biology 385.2 (2014 Jan 15): 263-78.

    Gene expression patterns : GEP
    De Robertis EM
    Gene expression patterns : GEP 11.7 (2011 Oct): 456-63.

    Genetics
    Rebay I
    Genetics 193.2 (2013 Feb): 633-49.

    Proceedings of the National Academy of Sciences of the United States of America
    Keshishian H
    Proceedings of the National Academy of Sciences of the United States of America 98.22 (2001 Oct 23): 12596-601.

    Cell
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    Journal of neurophysiology
    Levine RB
    Journal of neurophysiology 107.5 (2012 Mar): 1356-65.

    Development (Cambridge, England)
    Doe CQ
    Development (Cambridge, England) 138.9 (2011 May): 1727-35.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Keshishian H
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.24 (2004 Jun 16): 5482-91.

    Gene expression patterns : GEP
    Doe CQ
    Gene expression patterns : GEP 12.1-2 (2012 Jan-Feb): 11-7.

    Nature methods
    Stern DL
    Nature methods 10.8 (2013 Aug): 762-7.

    The Journal of comparative neurology
    Kjaerulff O
    The Journal of comparative neurology 517.3 (2009 Nov 20): 313-32.

    Molecular and cellular biology
    Rebay I
    Molecular and cellular biology 30.5 (2010 Mar): 1158-70.

    Development (Cambridge, England)
    Datta S
    Development (Cambridge, England) 128.10 (2001 May): 1899-909.

    Journal of biomechanics
    Brand RA
    Journal of biomechanics 33.1 (2000 Jan): 63-71.

    Developmental biology
    Sibon OC
    Developmental biology 313.2 (2008 Jan 15): 802-13.

    Development (Cambridge, England)
    Johnson WA
    Development (Cambridge, England) 127.11 (2000 Jun): 2395-405.

    Development (Cambridge, England)
    Terman JR
    Development (Cambridge, England) 134.12 (2007 Jun): 2337-47.

    Neural development
    Wyman RJ
    Neural development 4. (2009 Aug 17): 31.

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