9.4A anti-Trio

(0) No Reviews yet
SKU: 9.4A anti-Trio
View product citations for antibody 9.4A anti-Trio on CiteAb

In Stock

Available: 95

DSHB Data Sheet

Catalog Fields

Antigen: Trio
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Hama, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Faculty of Life Sciences, Kyoto Sangyo University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 9.4A stains the gamma and alpha’/beta’ lobes of mushroom body.
Antigen Molecular Weight: Multiple isoforms; Predicted: 46, 73, 88, and 257 kDa; Apparent: 130 and 257 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: trio
Immunogen: Recombinant Trio (aa 1697–1925 of isoform A)
Alternate Gene Names: CG18214
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-U1
Epitope Location or Sequence: aa 1697–1925 of isoform A
Uniprot ID: Q7KVD1 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43974 
Additional Characterization:  
Antibody Registry ID: AB_528494 
Additional Information: This antibody should recognize isoforms A and D and may recognize isoforms B and E.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.4A anti-Trio was deposited to the DSHB by Hama, C. (DSHB Hybridoma Product 9.4A anti-Trio)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review