9.4A anti-Trio

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SKU: 9.4A anti-Trio
View product citations for antibody 9.4A anti-Trio on CiteAb

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Available: 69

DSHB Data Sheet

Catalog Fields

Antigen: Trio
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Hama, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kyoto Sangyo University, Faculty of Life Sciences
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 9.4A stains the gamma and alpha’/beta’ lobes of mushroom body.
Antigen Molecular Weight: Multiple isoforms; Predicted: 46, 73, 88, and 257 kDa; Apparent: 130 and 257 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: trio
Immunogen: Recombinant Trio (aa 1697–1925 of isoform A)
Alternate Gene Names: CG18214
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-U1
Epitope Location or Sequence: aa 1697–1925 of isoform A
Uniprot ID: Q7KVD1 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43974 
Additional Characterization:  
Antibody Registry ID: AB_528494 
Additional Information: This antibody should recognize isoforms A and D and may recognize isoforms B and E.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.4A anti-Trio was deposited to the DSHB by Hama, C. (DSHB Hybridoma Product 9.4A anti-Trio)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The Drosophila trio plays an essential role in patterning of axons by regulating their directional extension.
    Hama C
    Neuron 26.1 (2000 Apr): 119-31.

    L1CAM/Neuroglian controls the axon-axon interactions establishing layered and lobular mushroom body architecture.
    Pielage J
    The Journal of cell biology 208.7 (2015 Mar 30): 1003-18.

    The TRIM-NHL protein Brat promotes axon maintenance by repressing src64B expression.
    Besse F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.41 (2014 Oct 8): 13855-64.

    Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitors.
    Bray S
    Journal of cell science 127.Pt 21 (2014 Nov 1): 4634-44.

    Retrograde BMP signaling controls synaptic growth at the NMJ by regulating trio expression in motor neurons.
    Haghighi AP
    Neuron 66.4 (2010 May 27): 536-49.

    The RhoGEF trio functions in sculpting class specific dendrite morphogenesis in Drosophila sensory neurons.
    Cox DN
    PloS one 7.3 (2012): e33634.

    Precise control of fasciclin II expression is required for adult mushroom body development in Drosophila.
    Tsujimura H
    Development, growth & differentiation 49.3 (2007 Apr): 215-27.

    Rac1 modulation of the apical domain is negatively regulated by β (Heavy)-spectrin.
    Thomas GH
    Mechanisms of development 128.1-2 (2011 Jan-Feb): 116-28.

    Glia-derived temporal signals orchestrate neurogenesis in the <i>Drosophila</i> mushroom body.
    Ho MS
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

    WB References
    Epitope Map References
    All References

    The Drosophila trio plays an essential role in patterning of axons by regulating their directional extension.
    Hama C
    Neuron 26.1 (2000 Apr): 119-31.

    L1CAM/Neuroglian controls the axon-axon interactions establishing layered and lobular mushroom body architecture.
    Pielage J
    The Journal of cell biology 208.7 (2015 Mar 30): 1003-18.

    The TRIM-NHL protein Brat promotes axon maintenance by repressing src64B expression.
    Besse F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.41 (2014 Oct 8): 13855-64.

    Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitors.
    Bray S
    Journal of cell science 127.Pt 21 (2014 Nov 1): 4634-44.

    Retrograde BMP signaling controls synaptic growth at the NMJ by regulating trio expression in motor neurons.
    Haghighi AP
    Neuron 66.4 (2010 May 27): 536-49.

    The RhoGEF trio functions in sculpting class specific dendrite morphogenesis in Drosophila sensory neurons.
    Cox DN
    PloS one 7.3 (2012): e33634.

    Precise control of fasciclin II expression is required for adult mushroom body development in Drosophila.
    Tsujimura H
    Development, growth & differentiation 49.3 (2007 Apr): 215-27.

    Rac1 modulation of the apical domain is negatively regulated by β (Heavy)-spectrin.
    Thomas GH
    Mechanisms of development 128.1-2 (2011 Jan-Feb): 116-28.

    Glia-derived temporal signals orchestrate neurogenesis in the <i>Drosophila</i> mushroom body.
    Ho MS
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

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