Catalog Fields
Antigen: Trio
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Hama, C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Kyoto Sangyo University, Faculty of Life Sciences
Antigen Molecular Weight: Multiple isoforms; Predicted: 46, 73, 88, and 257 kDa; Apparent: 130 and 257 kDa
Depositors Notes: 9.4A stains the gamma and alpha’/beta’ lobes of mushroom body.
Immunogen: Recombinant Trio (aa 1697–1925 of isoform A)
Gene: trio
Alternate Antibody Name:
Alternate Gene Names: CG18214
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3-X63-Ag8-U1
Epitope Mapped: Yes
Uniprot ID: Q7KVD1
Epitope Location or Sequence: aa 1697–1925 of isoform A
Entrez Gene ID: 43974
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528494
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: This antibody should recognize isoforms A and D and may recognize isoforms B and E.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.4A anti-Trio was deposited to the DSHB by Hama, C. (DSHB Hybridoma Product 9.4A anti-Trio)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
The Drosophila trio plays an essential role in patterning of axons by regulating their directional extension.
Hama C
Neuron 26.1 (2000 Apr): 119-31.