9B2.1 anti-glass

(0) No Reviews yet
SKU: 9B2.1 anti-glass
View product citations for antibody 9B2.1 anti-glass on CiteAb

In Stock

Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Glass
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California - Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 9B2.1 Ab recognizes the Drosophila protein glass. Glass is expressed at high levels in the nucleus of the developing photoreceptor cells in a sequence that reflects their acquisition of neuronal identity. 9B2.1 gives strong staining of the morphogenetic furrow and all of the cells that arise from the furrow.
Antigen Molecular Weight: 62.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: gl
Immunogen: Glass-trp E fusion protein (see PMID: 2010085)
Alternate Gene Names: CG7672
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3 x 63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P13360 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 42210 
Additional Characterization:  
Antibody Registry ID: AB_528268 
Additional Information: RRID: AB_528268. FBgn0004618. Use periodate-lysine-paraformaldehyde (PLP) fix only.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9B2.1 anti-glass was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 9B2.1 anti-glass)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review