9B8 anti-sidestep

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$45.00 to $425.00
SKU: 9B8 anti-sidestep
View product citations for antibody 9B8 anti-sidestep on CiteAb

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Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: sidestep
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Goodman, C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of California, Berkeley
Antigen Molecular Weight: Predicted: multiple isoforms between 100 and 107 kDa; Apparent: around 120 kDa
Depositors Notes: Works better on fresh rather than MeOH stored embryos, stronger CNS, weaker muscle staining, Best results with use of VectaStain kit .
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant 6× His-tagged sidestep protein fragment (aa 241–786)
Gene: side
Alternate Antibody Name: 9B8
Alternate Gene Names: CG31062
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3 x 63 Ag8.653
Epitope Mapped: Yes
Uniprot ID: Q9VB35 
Epitope Location or Sequence: aa 241–786 (extracellular)
Entrez Gene ID: 43300 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528468 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9B8 anti-sidestep was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 9B8 anti-sidestep)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
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