9B8 anti-sidestep

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SKU: 9B8 anti-sidestep
View product citations for antibody 9B8 anti-sidestep on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: sidestep
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Works better on fresh rather than MeOH stored embryos, stronger CNS, weaker muscle staining, Best results with use of VectaStain kit .
Antigen Molecular Weight: Predicted: multiple isoforms between 100 and 107 kDa; Apparent: around 120 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: side
Immunogen: Recombinant 6× His-tagged sidestep protein fragment (aa 241–786)
Alternate Gene Names: CG31062
Alternate Antibody Name: 9B8
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence: aa 241–786 (extracellular)
Uniprot ID: Q9VB35 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43300 
Additional Characterization:  
Antibody Registry ID: AB_528468 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9B8 anti-sidestep was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 9B8 anti-sidestep)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
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