AD4.4(EcR-B1)

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$40.00
SKU: AD4.4(EcR-B1)
View product citations for antibody AD4.4(EcR-B1) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR-B1)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Thummel, C. / Hogness, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Utah
Positive Tested Species Reactivity: Drosophila, Sciara coprophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 94 kDa; Apparent: 105 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: EcR
Immunogen: TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).
Alternate Gene Names: NR1H1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: aa 68-222
Uniprot ID: P34021 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35540 
Additional Characterization:
Antibody Registry ID: AB_2154902 
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AD4.4(EcR-B1) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product AD4.4(EcR-B1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
    Hogness DS
    Cell 73.7 (1993 Jul 2): 1323-37.

    The ecdysone receptor signalling regulates microvilli formation in follicular epithelial cells.
    Cavaliere V
    Cellular and molecular life sciences : CMLS 73.2 (2016 Jan): 409-25.

    Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function.
    Sokol NS
    Development (Cambridge, England) 139.10 (2012 May): 1788-97.

    EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis.
    Cavaliere V
    Developmental biology 328.2 (2009 Apr 15): 541-51.

    Ecdysone receptor expression in the CNS correlates with stage-specific responses to ecdysteroids during Drosophila and Manduca development.
    Hogness DS
    Development (Cambridge, England) 120.1 (1994 Jan): 219-34.

    The ecdysone receptor and ultraspiracle regulate the timing and progression of ovarian morphogenesis during Drosophila metamorphosis.
    Riddiford LM
    Development genes and evolution 208.6 (1998 Aug): 304-17.

    Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor.
    Truman JW
    Mechanisms of development 120.8 (2003 Aug): 909-18.

    Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis.
    Bender M
    Development (Cambridge, England) 125.11 (1998 Jun): 2053-62.

    The Drosophila ecdysone receptor (EcR) gene is required maternally for normal oogenesis.
    Bender M
    Genetics 154.3 (2000 Mar): 1203-11.

    Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila.
    Luo L
    Neuron 28.3 (2000 Dec): 807-18.

    The dual role of ultraspiracle, the Drosophila retinoid X receptor, in the ecdysone response.
    McKeown M
    Proceedings of the National Academy of Sciences of the United States of America 98.7 (2001 Mar 27): 3867-72.

    Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
    Cavaliere V
    Genetics 181.1 (2009 Jan): 165-75.

    WB References
    IHC References
    Epitope Map References
    Gel Supershift References
    All References

    Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
    Hogness DS
    Cell 73.7 (1993 Jul 2): 1323-37.

    Ecdysone receptor expression and activity in adult Drosophila melanogaster.
    Carney GE
    Journal of insect physiology 57.7 (2011 Jul): 899-907.

    Ecdysone receptor expression in the CNS correlates with stage-specific responses to ecdysteroids during Drosophila and Manduca development.
    Hogness DS
    Development (Cambridge, England) 120.1 (1994 Jan): 219-34.

    The ecdysone receptor signalling regulates microvilli formation in follicular epithelial cells.
    Cavaliere V
    Cellular and molecular life sciences : CMLS 73.2 (2016 Jan): 409-25.

    Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function.
    Sokol NS
    Development (Cambridge, England) 139.10 (2012 May): 1788-97.

    EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis.
    Cavaliere V
    Developmental biology 328.2 (2009 Apr 15): 541-51.

    The ecdysone receptor and ultraspiracle regulate the timing and progression of ovarian morphogenesis during Drosophila metamorphosis.
    Riddiford LM
    Development genes and evolution 208.6 (1998 Aug): 304-17.

    Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor.
    Truman JW
    Mechanisms of development 120.8 (2003 Aug): 909-18.

    Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis.
    Bender M
    Development (Cambridge, England) 125.11 (1998 Jun): 2053-62.

    The Drosophila ecdysone receptor (EcR) gene is required maternally for normal oogenesis.
    Bender M
    Genetics 154.3 (2000 Mar): 1203-11.

    Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila.
    Luo L
    Neuron 28.3 (2000 Dec): 807-18.

    The dual role of ultraspiracle, the Drosophila retinoid X receptor, in the ecdysone response.
    McKeown M
    Proceedings of the National Academy of Sciences of the United States of America 98.7 (2001 Mar 27): 3867-72.

    Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
    Cavaliere V
    Genetics 181.1 (2009 Jan): 165-75.

    An arginine-histone methyltransferase, CARMER, coordinates ecdysone-mediated apoptosis in Drosophila cells.
    Kumar S
    The Journal of biological chemistry 279.18 (2004 Apr 30): 18467-71.

    DNA binding properties of the ecdysteroid receptor in the salivary gland of the female ixodid tick, Amblyomma hebraeum.
    Kaufman WR
    Insect biochemistry and molecular biology 28.12 (1998 Dec): 947-57.

    Ecdysone induces transcription and amplification in Sciara coprophila DNA puff II/9A.
    Gerbi SA
    Developmental biology 299.1 (2006 Nov 1): 151-63.

    Programmed cell death in the Drosophila CNS is ecdysone-regulated and coupled with a specific ecdysone receptor isoform.
    Truman JW
    Development (Cambridge, England) 119.4 (1993 Dec): 1251-9.

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