AD4.4(EcR-B1)
Catalog Fields
Antigen: ecdysone receptor (EcR-B1)
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Thummel, C. / Hogness, D.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Sciara coprophila
Depositors Institution: University of Utah
Antigen Molecular Weight: Predicted: 94 kDa; Apparent: 105 kDa
Depositors Notes:
Immunogen: TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).
Gene: EcR
Alternate Antibody Name:
Alternate Gene Names: NR1H1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P34021
Epitope Location or Sequence: aa 68-222
Entrez Gene ID: 35540
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2154902
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AD4.4(EcR-B1) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product AD4.4(EcR-B1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
18 References
Initial Publication
Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
Hogness DS
Cell 73.7 (1993 Jul 2): 1323-37.