Dally-like (13G8)
Catalog Fields
Antigen: Dally-like protein (Dlp)
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Beachy, P.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Stanford University
Antigen Molecular Weight: Predicted: 85 kDa
Depositors Notes:
Immunogen: Recombinant Drosophila Dally-like protein (aa 523-702)/human IgG Fc domain fusion
Gene: dlp
Alternate Antibody Name:
Alternate Gene Names: CG32146, dly, Dally-like
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: Yes
Uniprot ID: Q9VUG1
Epitope Location or Sequence: aa 523-702
Entrez Gene ID: 39596
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528191
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: Recognizes an extracellular epitope. Dally-like protein (Dlp) is a cell surface protein member of the glypican family of heparan sulfate proteoglycans (HSPGs).
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Dally-like (13G8) was deposited to the DSHB by Beachy, P.A. (DSHB Hybridoma Product Dally-like (13G8))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
Beachy PA
Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.