Dally-like (13G8)

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SKU: Dally-like (13G8)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Dally-like (13G8)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: dlp
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528191 
Uniprot ID: Q9VUG1 
RRID:  
Entrez Gene ID: 39596 
Clonality: Monoclonal
Immunogen: Recombinant Drosophila Dally-like protein (aa 523-702)/human IgG Fc domain fusion
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Dally-like protein (Dlp)
Epitope Location or Sequence: aa 523-702
Alternate Antigen Name:
Deposit Date: 10/21/2003
Antigen Molecular Weight: Predicted: 85 kDa
Depositor: Beachy, P.A.
Antigen Sequence:
Depositor Institution: Stanford University
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Recognizes an extracellular epitope. Dally-like protein (Dlp) is a cell surface protein member of the glypican family of heparan sulfate proteoglycans (HSPGs).
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Dally-like (13G8) was deposited to the DSHB by Beachy, P.A. (DSHB Hybridoma Product Dally-like (13G8))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    IF References

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    Heparan sulfate proteoglycan specificity during axon pathway formation in the Drosophila embryo.
    Johnson KG
    Developmental neurobiology 71.7 (2011 Jul): 608-18.

    Cellular trafficking of the glypican Dally-like is required for full-strength Hedgehog signaling and wingless transcytosis.
    Thérond PP
    Developmental cell 14.5 (2008 May): 712-25.

    Dally and Notum regulate the switch between low and high level Hedgehog pathway signalling.
    Gallet A
    Development (Cambridge, England) 139.17 (2012 Sep): 3168-79.

    Increased avidity for Dpp/BMP2 maintains the proliferation of progenitors-like cells in the Drosophila eye.
    Casares F
    Developmental biology 418.1 (2016 Oct 1): 98-107.

    Dally Proteoglycan Mediates the Autonomous and Nonautonomous Effects on Tissue Growth Caused by Activation of the PI3K and TOR Pathways.
    Milán M
    PLoS biology 13.8 (2015 Aug): e1002239.

    A matrix metalloproteinase mediates long-distance attenuation of stem cell proliferation.
    Page-McCaw A
    The Journal of cell biology 206.7 (2014 Sep 29): 923-36.

    Fragile X mental retardation protein regulates trans-synaptic signaling in Drosophila.
    Broadie K
    Disease models & mechanisms 6.6 (2013 Nov): 1400-13.

    A targeted glycan-related gene screen reveals heparan sulfate proteoglycan sulfation regulates WNT and BMP trans-synaptic signaling.
    Broadie K
    PLoS genetics 8.11 (2012): e1003031.

    Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs.
    Vincent JP
    Nature cell biology 18.4 (2016 Apr): 451-7.

    Two classes of matrix metalloproteinases reciprocally regulate synaptogenesis.
    Broadie K
    Development (Cambridge, England) 143.1 (2016 Jan 1): 75-87.

    Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
    Tanentzapf G
    Current biology : CB 30.17 (2020 Sep 7): 3316-3329.e5.

    WB References

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    Feedback regulation of Drosophila BMP signaling by the novel extracellular protein larval translucida.
    Gibson MC
    Development (Cambridge, England) 138.4 (2011 Feb): 715-24.

    IP References

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    Feedback regulation of Drosophila BMP signaling by the novel extracellular protein larval translucida.
    Gibson MC
    Development (Cambridge, England) 138.4 (2011 Feb): 715-24.

    ELISA References

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    Epitope Map References

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    All References

    Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
    Beachy PA
    Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.

    Heparan sulfate proteoglycan specificity during axon pathway formation in the Drosophila embryo.
    Johnson KG
    Developmental neurobiology 71.7 (2011 Jul): 608-18.

    Cellular trafficking of the glypican Dally-like is required for full-strength Hedgehog signaling and wingless transcytosis.
    Thérond PP
    Developmental cell 14.5 (2008 May): 712-25.

    Dally and Notum regulate the switch between low and high level Hedgehog pathway signalling.
    Gallet A
    Development (Cambridge, England) 139.17 (2012 Sep): 3168-79.

    Increased avidity for Dpp/BMP2 maintains the proliferation of progenitors-like cells in the Drosophila eye.
    Casares F
    Developmental biology 418.1 (2016 Oct 1): 98-107.

    Dally Proteoglycan Mediates the Autonomous and Nonautonomous Effects on Tissue Growth Caused by Activation of the PI3K and TOR Pathways.
    Milán M
    PLoS biology 13.8 (2015 Aug): e1002239.

    A matrix metalloproteinase mediates long-distance attenuation of stem cell proliferation.
    Page-McCaw A
    The Journal of cell biology 206.7 (2014 Sep 29): 923-36.

    Fragile X mental retardation protein regulates trans-synaptic signaling in Drosophila.
    Broadie K
    Disease models & mechanisms 6.6 (2013 Nov): 1400-13.

    A targeted glycan-related gene screen reveals heparan sulfate proteoglycan sulfation regulates WNT and BMP trans-synaptic signaling.
    Broadie K
    PLoS genetics 8.11 (2012): e1003031.

    Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs.
    Vincent JP
    Nature cell biology 18.4 (2016 Apr): 451-7.

    Two classes of matrix metalloproteinases reciprocally regulate synaptogenesis.
    Broadie K
    Development (Cambridge, England) 143.1 (2016 Jan 1): 75-87.

    Integrins Modulate Extracellular Matrix Organization to Control Cell Signaling during Hematopoiesis.
    Tanentzapf G
    Current biology : CB 30.17 (2020 Sep 7): 3316-3329.e5.

    Feedback regulation of Drosophila BMP signaling by the novel extracellular protein larval translucida.
    Gibson MC
    Development (Cambridge, England) 138.4 (2011 Feb): 715-24.

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