Dally-like (13G8)
Catalog Fields
Antigen: Dally-like protein (Dlp)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Beachy, P.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Predicted Species Reactivity:
Gene: dlp
Immunogen: Recombinant Drosophila Dally-like protein (aa 523-702)/human IgG Fc domain fusion
Alternate Gene Names: CG32146, dly, Dally-like
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 523-702
Uniprot ID: Q9VUG1
Immunogen Sequence: Partial protein
Entrez Gene ID: 39596
Antibody Registry ID: AB_528191
Additional Information: Recognizes an extracellular epitope. Dally-like protein (Dlp) is a cell surface protein member of the glypican family of heparan sulfate proteoglycans (HSPGs).
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Dally-like (13G8) was deposited to the DSHB by Beachy, P.A. (DSHB Hybridoma Product Dally-like (13G8))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.
Beachy PA
Science (New York, N.Y.) 299.5615 (2003 Mar 28): 2039-45.