ESC 53.1

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$40.00
SKU: ESC 53.1
View product citations for antibody ESC 53.1 on CiteAb

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Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: Extra Sex Combs/Polycomb protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge - UK
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 47.9 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: esc
Immunogen: Recombinant N-terminus of ESC protein excluding WD-40 domains
Alternate Gene Names: Dmel_CG14941
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NSO
Epitope Location or Sequence:
Uniprot ID: Q24338 
Immunogen Sequence:
Entrez Gene ID: 34595 
Additional Characterization:
Antibody Registry ID: AB_10659720 
Additional Information: RRID: AB_10659720 FBgn0000588.
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ESC 53.1 was deposited to the DSHB by White, R. (DSHB Hybridoma Product ESC 53.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication
    WB References
    IHC References
    All References

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