ESC 53.1

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$40.00
SKU: ESC 53.1
View product citations for antibody ESC 53.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Extra Sex Combs/Polycomb protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge - UK
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 47.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: esc
Immunogen: Recombinant N-terminus of ESC protein excluding WD-40 domains
Alternate Gene Names: Dmel_CG14941
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NSO
Epitope Location or Sequence:
Uniprot ID: Q24338 
Immunogen Sequence:
Entrez Gene ID: 34595 
Additional Characterization:  
Antibody Registry ID: AB_10659720 
Additional Information: RRID: AB_10659720 FBgn0000588.
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ESC 53.1 was deposited to the DSHB by White, R. (DSHB Hybridoma Product ESC 53.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication
    WB References
    IHC References
    All References

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