6E8

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$40.00
SKU: 6E8
View product citations for antibody 6E8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Posterior sex combs protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Adler, P.N.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virgina
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 169.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Psc
Immunogen: B-galactosidase -N-terminus fusion protein
Alternate Gene Names: Dmel_CG3886
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp 2/0 - Ag 14
Epitope Location or Sequence:
Uniprot ID: P35820 
Immunogen Sequence: a.a. 1-300
Entrez Gene ID: 36431 
Additional Characterization:
Antibody Registry ID: AB_528437 
Additional Information: RRID:AB_528437. FBgn0005624.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6E8 was deposited to the DSHB by Adler, P.N. (DSHB Hybridoma Product 6E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    The Polycomb group gene Posterior Sex Combs encodes a chromosomal protein.
    Adler PN
    Development (Cambridge, England) 117.2 (1993 Feb): 641-55.

    IF References

    pipsqueak encodes a factor essential for sequence-specific targeting of a polycomb group protein complex.
    King B
    Molecular and cellular biology 22.17 (2002 Sep): 6261-71.

    Essential role of Drosophila Hdac1 in homeotic gene silencing.
    Huang DH
    Proceedings of the National Academy of Sciences of the United States of America 98.17 (2001 Aug 14): 9730-5.

    WB References
    All References

    pipsqueak encodes a factor essential for sequence-specific targeting of a polycomb group protein complex.
    King B
    Molecular and cellular biology 22.17 (2002 Sep): 6261-71.

    Essential role of Drosophila Hdac1 in homeotic gene silencing.
    Huang DH
    Proceedings of the National Academy of Sciences of the United States of America 98.17 (2001 Aug 14): 9730-5.

    The Polycomb group gene Posterior Sex Combs encodes a chromosomal protein.
    Adler PN
    Development (Cambridge, England) 117.2 (1993 Feb): 641-55.

    A Drosophila Polycomb group complex includes Zeste and dTAFII proteins.
    Kingston RE
    Nature 412.6847 (2001 Aug 9): 655-60.

    Analysis of a polycomb group protein defines regions that link repressive activity on nucleosomal templates to in vivo function.
    Wu CT
    Molecular and cellular biology 25.15 (2005 Aug): 6578-91.

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