Ubx FP3.38
Catalog Fields
Antigen: Ultrabithorax 5' exon (107 amino acids)
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: White, R.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila melanogaster, Drosophila simulans
Depositors Institution: University of Cambridge
Antigen Molecular Weight: Predicted: multiple isoforms, from 35 to 40kDa
Depositors Notes: Limited species specificity
Immunogen: Recombinant Drosophila Ultrabithorax 5’ exon (107 aa)/lacZ fusion protein
Gene: Ubx
Alternate Antibody Name: FP3.38
Alternate Gene Names: CG10388, bx, Cbx, pbx, abx, bxd, DmUbx
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NSO
Epitope Mapped: Yes
Uniprot ID: P83949
Epitope Location or Sequence: N-terminal region encoded by the 5' exon (aa 1-107)
Entrez Gene ID: 42034
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_10805300
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: The N-terminal region containing the epitope recognized by Ubx FP3.38 is common to all isoforms
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ubx FP3.38 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Ubx FP3.38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Protein products of the bithorax complex in Drosophila.
Wilcox M
Cell 39.1 (1984 Nov): 163-71.