Ubx FP3.38

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$40.00
SKU: Ubx FP3.38
View product citations for antibody Ubx FP3.38 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Ultrabithorax 5' exon (107 amino acids)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Drosophila melanogaster, Drosophila simulans
Depositors Notes: Limited species specificity
Antigen Molecular Weight: Predicted: multiple isoforms, from 35 to 40kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ubx
Immunogen: Recombinant Drosophila Ultrabithorax 5’ exon (107 aa)/lacZ fusion protein
Alternate Gene Names: CG10388, bx, Cbx, pbx, abx, bxd, DmUbx
Alternate Antibody Name: FP3.38
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: N-terminal region encoded by the 5' exon (aa 1-107)
Uniprot ID: P83949 
Immunogen Sequence: Partial protein
Entrez Gene ID: 42034 
Additional Characterization:  
Antibody Registry ID: AB_10805300 
Additional Information: The N-terminal region containing the epitope recognized by Ubx FP3.38 is common to all isoforms
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ubx FP3.38 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Ubx FP3.38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Protein products of the bithorax complex in Drosophila.
    Wilcox M
    Cell 39.1 (1984 Nov): 163-71.

    IF References

    Protein products of the bithorax complex in Drosophila.
    Wilcox M
    Cell 39.1 (1984 Nov): 163-71.

    A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox/PBC interaction mode.
    Graba Y
    Structure (London, England : 1993) 23.2 (2015 Feb 3): 270-9.

    Impact of Ultrabithorax alternative splicing on Drosophila embryonic nervous system development.
    Rogulja-Ortmann A
    Mechanisms of development 138 Pt 2. (2015 Nov): 177-189.

    Distinct Roles of Chromatin Insulator Proteins in Control of the Drosophila Bithorax Complex.
    Schwartz YB
    Genetics 202.2 (2016 Feb): 601-17.

    Variability in the number of abdominal leucokinergic neurons in adult Drosophila melanogaster.
    Herrero P
    The Journal of comparative neurology 525.3 (2017 Feb 15): 639-660.

    New insights for Drosophila GAGA factor in larvae.
    Bernués J
    Royal Society open science 2.3 (2015 Mar): 150011.

    Maintenance of Tissue Pluripotency by Epigenetic Factors Acting at Multiple Levels.
    Huang DH
    PLoS genetics 12.2 (2016 Feb): e1005897.

    Inhibitory activities of short linear motifs underlie Hox interactome specificity in vivo.
    Merabet S
    eLife 4. (2015 Apr 14): .

    Evolutionarily conserved domains required for activation and repression functions of the Drosophila Hox protein Ultrabithorax.
    McGinnis W
    Development (Cambridge, England) 132.23 (2005 Dec): 5271-81.

    Hox gene Ultrabithorax regulates distinct sets of target genes at successive stages of Drosophila haltere morphogenesis.
    Akam M
    Proceedings of the National Academy of Sciences of the United States of America 108.7 (2011 Feb 15): 2855-60.

    Structural basis for targeting the chromatin repressor Sfmbt to Polycomb response elements.
    Müller CW
    Genes & development 27.21 (2013 Nov 1): 2367-79.

    A role of Ultrabithorax in morphological differences between Drosophila species.
    Stern DL
    Nature 396.6710 (1998 Dec 3): 463-6.

    WB References

    Protein products of the bithorax complex in Drosophila.
    Wilcox M
    Cell 39.1 (1984 Nov): 163-71.

    Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic.
    Bondos SE
    Journal of biomedical materials research. Part A 103.4 (2015 Apr): 1546-53.

    Impact of Ultrabithorax alternative splicing on Drosophila embryonic nervous system development.
    Rogulja-Ortmann A
    Mechanisms of development 138 Pt 2. (2015 Nov): 177-189.

    Phosphorylation, expression and function of the Ultrabithorax protein family in Drosophila melanogaster.
    Hogness DS
    Development (Cambridge, England) 112.4 (1991 Aug): 1077-93.

    Homeotic control in Drosophila; the scabrous gene is an in vivo target of Ultrabithorax proteins.
    Pradel J
    The EMBO journal 11.9 (1992 Sep): 3375-84.

    IHC References
    Epitope Map References

    Protein products of the bithorax complex in Drosophila.
    Wilcox M
    Cell 39.1 (1984 Nov): 163-71.

    All References

    Drosophila Cyclin G and epigenetic maintenance of gene expression during development.
    Peronnet F
    Epigenetics & chromatin 8. (2015): 18.

    A Single MicroRNA-Hox Gene Module Controls Equivalent Movements in Biomechanically Distinct Forms of Drosophila.
    Alonso CR
    Current biology : CB 29.16 (2019 Aug 19): 2665-2675.e4.

    Protein products of the bithorax complex in Drosophila.
    Wilcox M
    Cell 39.1 (1984 Nov): 163-71.

    A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox/PBC interaction mode.
    Graba Y
    Structure (London, England : 1993) 23.2 (2015 Feb 3): 270-9.

    Impact of Ultrabithorax alternative splicing on Drosophila embryonic nervous system development.
    Rogulja-Ortmann A
    Mechanisms of development 138 Pt 2. (2015 Nov): 177-189.

    Distinct Roles of Chromatin Insulator Proteins in Control of the Drosophila Bithorax Complex.
    Schwartz YB
    Genetics 202.2 (2016 Feb): 601-17.

    Variability in the number of abdominal leucokinergic neurons in adult Drosophila melanogaster.
    Herrero P
    The Journal of comparative neurology 525.3 (2017 Feb 15): 639-660.

    New insights for Drosophila GAGA factor in larvae.
    Bernués J
    Royal Society open science 2.3 (2015 Mar): 150011.

    Maintenance of Tissue Pluripotency by Epigenetic Factors Acting at Multiple Levels.
    Huang DH
    PLoS genetics 12.2 (2016 Feb): e1005897.

    Inhibitory activities of short linear motifs underlie Hox interactome specificity in vivo.
    Merabet S
    eLife 4. (2015 Apr 14): .

    Evolutionarily conserved domains required for activation and repression functions of the Drosophila Hox protein Ultrabithorax.
    McGinnis W
    Development (Cambridge, England) 132.23 (2005 Dec): 5271-81.

    Hox gene Ultrabithorax regulates distinct sets of target genes at successive stages of Drosophila haltere morphogenesis.
    Akam M
    Proceedings of the National Academy of Sciences of the United States of America 108.7 (2011 Feb 15): 2855-60.

    Structural basis for targeting the chromatin repressor Sfmbt to Polycomb response elements.
    Müller CW
    Genes & development 27.21 (2013 Nov 1): 2367-79.

    A role of Ultrabithorax in morphological differences between Drosophila species.
    Stern DL
    Nature 396.6710 (1998 Dec 3): 463-6.

    Materials composed of the Drosophila Hox protein Ultrabithorax are biocompatible and nonimmunogenic.
    Bondos SE
    Journal of biomedical materials research. Part A 103.4 (2015 Apr): 1546-53.

    Phosphorylation, expression and function of the Ultrabithorax protein family in Drosophila melanogaster.
    Hogness DS
    Development (Cambridge, England) 112.4 (1991 Aug): 1077-93.

    Homeotic control in Drosophila; the scabrous gene is an in vivo target of Ultrabithorax proteins.
    Pradel J
    The EMBO journal 11.9 (1992 Sep): 3375-84.

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