EXD B11M
Catalog Fields
Product Name/ID: EXD B11M
Available to For-Profits: Yes
Alternate Antibody Name: B11M
Gene Name: exd
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_10683835
Uniprot ID: P40427
Entrez Gene ID: 32567
Clonality: Monoclonal
Immunogen: Recombinant EXD (lacking 150 aa at the N-terminus) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NSO
Epitope Mapped: Yes
Antigen Name: Extradenticle homeobox protein (Dpbx)
Epitope Location or Sequence: aa 150-376
Alternate Antigen Name:
Deposit Date: 5/25/2010
Antigen Molecular Weight: Predicted: 42 kDa
Depositor: White, R.
Antigen Sequence:
Depositor Institution: University of Cambridge
Antigen Species: drosophila
Depositor Notes: Doesn't label exd- mutant embryos.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bee, Crustacean, Drosophila, Locust, Sepsidae, Spider
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EXD B11M was deposited to the DSHB by White, R. (DSHB Hybridoma Product EXD B11M)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Nucleocytoplasmic localisation of extradenticle protein is spatially regulated throughout development in Drosophila.
White RA
Development (Cambridge, England) 124.3 (1997 Feb): 741-7.