EXD B11M

(0) No Reviews yet
$40.00
SKU: EXD B11M
View product citations for antibody EXD B11M on CiteAb

In Stock

Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Extradenticle homeobox protein (Dpbx)
Hybridoma Cells Available: Yes
Antigen Species: drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge - UK
Positive Tested Species Reactivity: Bee, Crustacean, Drosophila, Locust, Sepsidae, Spider
Depositors Notes: Doesn't label exd- mutant embryos.
Antigen Molecular Weight: Predicted: 42 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: exd
Immunogen: Recombinant EXD (lacking 150 aa at the N-terminus) fusion protein
Alternate Gene Names: Dpbx, CG8933, Pbx1
Alternate Antibody Name: B11M
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 150-376
Uniprot ID: P40427 
Immunogen Sequence: Partial protein
Entrez Gene ID: 32567 
Additional Characterization:  
Antibody Registry ID: AB_10683835 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EXD B11M was deposited to the DSHB by White, R. (DSHB Hybridoma Product EXD B11M)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Nucleocytoplasmic localisation of extradenticle protein is spatially regulated throughout development in Drosophila.
    White RA
    Development (Cambridge, England) 124.3 (1997 Feb): 741-7.

    Evidence for a novel role for dachshund in patterning the proximal arthropod leg.
    Nagy L
    Development genes and evolution 218.6 (2008 Jun): 293-305.

    Partial co-option of the appendage patterning pathway in the development of abdominal appendages in the sepsid fly Themira biloba.
    Nijhout HF
    Development genes and evolution 219.11-12 (2009 Dec): 577-87.

    Mutational analysis of the Drosophila homothorax gene.
    Salzberg A
    Genetics 157.2 (2001 Feb): 689-98.

    A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species.
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Genetic evidence for the transcriptional-activating function of Homothorax during adult fly development.
    Salzberg A
    Development (Cambridge, England) 128.18 (2001 Sep): 3405-13.

    Comparative analysis of Wingless patterning in the embryonic grasshopper eye.
    Friedrich M
    Development genes and evolution 215.4 (2005 Apr): 177-97.

    Homeotic functions of the Teashirt transcription factor during adult Drosophila development.
    Yoder JH
    Biology open 2.1 (2013 Jan 15): 18-29.

    Homothorax plays autonomous and nonautonomous roles in proximodistal axis formation and migration of the Drosophila renal tubules.
    Salzberg A
    Developmental dynamics : an official publication of the American Association of Anatomists 243.1 (2014 Jan): 132-44.

    Segment-specific neuronal subtype specification by the integration of anteroposterior and temporal cues.
    Thor S
    PLoS biology 8.5 (2010 May 11): e1000368.

    Roles of cofactors and chromatin accessibility in Hox protein target specificity.
    White R
    Epigenetics & chromatin 9. (2016): 1.

    Increased avidity for Dpp/BMP2 maintains the proliferation of progenitors-like cells in the Drosophila eye.
    Casares F
    Developmental biology 418.1 (2016 Oct 1): 98-107.

    WB References
    IHC References
    Epitope Map References
    All References

    Nucleocytoplasmic localisation of extradenticle protein is spatially regulated throughout development in Drosophila.
    White RA
    Development (Cambridge, England) 124.3 (1997 Feb): 741-7.

    Dorsotonals/homothorax, the Drosophila homologue of meis1, interacts with extradenticle in patterning of the embryonic PNS.
    Salzberg A
    Development (Cambridge, England) 125.6 (1998 Mar): 1037-48.

    Evidence for a novel role for dachshund in patterning the proximal arthropod leg.
    Nagy L
    Development genes and evolution 218.6 (2008 Jun): 293-305.

    Partial co-option of the appendage patterning pathway in the development of abdominal appendages in the sepsid fly Themira biloba.
    Nijhout HF
    Development genes and evolution 219.11-12 (2009 Dec): 577-87.

    Mutational analysis of the Drosophila homothorax gene.
    Salzberg A
    Genetics 157.2 (2001 Feb): 689-98.

    A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species.
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Genetic evidence for the transcriptional-activating function of Homothorax during adult fly development.
    Salzberg A
    Development (Cambridge, England) 128.18 (2001 Sep): 3405-13.

    Comparative analysis of Wingless patterning in the embryonic grasshopper eye.
    Friedrich M
    Development genes and evolution 215.4 (2005 Apr): 177-97.

    Homeotic functions of the Teashirt transcription factor during adult Drosophila development.
    Yoder JH
    Biology open 2.1 (2013 Jan 15): 18-29.

    Homothorax plays autonomous and nonautonomous roles in proximodistal axis formation and migration of the Drosophila renal tubules.
    Salzberg A
    Developmental dynamics : an official publication of the American Association of Anatomists 243.1 (2014 Jan): 132-44.

    Segment-specific neuronal subtype specification by the integration of anteroposterior and temporal cues.
    Thor S
    PLoS biology 8.5 (2010 May 11): e1000368.

    Roles of cofactors and chromatin accessibility in Hox protein target specificity.
    White R
    Epigenetics & chromatin 9. (2016): 1.

    Increased avidity for Dpp/BMP2 maintains the proliferation of progenitors-like cells in the Drosophila eye.
    Casares F
    Developmental biology 418.1 (2016 Oct 1): 98-107.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review