Flamingo #74
Catalog Fields
Antigen: Flamingo (starry night)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Uemura, T.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kyoto Univ. Graduate Sch.of Biostudies
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen.
Predicted Species Reactivity:
Gene: stan
Immunogen: Extracellular domain, aa 539-1219.
Alternate Gene Names: CG11895, fmi, flam
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-U1
Epitope Location or Sequence: Extracellular domain, aa 539-1219 (see depositor notes).
Uniprot ID: Q9V5N8
Immunogen Sequence: Partial protein
Entrez Gene ID: 36125
Antibody Registry ID: AB_2619583
Additional Information: Flamingo is the protocadherin-like wing polarity protein stan.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Flamingo #74 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product Flamingo #74)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled.
Uemura T
Cell 98.5 (1999 Sep 3): 585-95.