Clone ID / Name: Flamingo #74
Anti - Flamingo (starry night)
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: P3-X63-Ag8-U1
Antibody Registry / RRID: AB_2619583
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Extracellular domain, aa 539-1219.
Immunogen Sequence: Partial protein
Epitope Mapped: Yes
Epitope Location / Seq: Extracellular domain, aa 539-1219 (see depositor notes).
Clone: —
Additional Info: Flamingo is the protocadherin-like wing polarity protein stan.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Drosophila
Predicted Reactivity: —
Depositor
Depositor Name: T. Uemura
Institution: Kyoto Univ. Graduate Sch.of Biostudies
Deposit Date: January 7, 2004
Depositor Notes: Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen.
Recommended Applications
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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