Flamingo #74

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$40.00
SKU: Flamingo #74
View product citations for antibody Flamingo #74 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Flamingo (starry night)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Uemura, T.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kyoto Univ. Graduate Sch.of Biostudies
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen.
Antigen Molecular Weight: Predicted: 394 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: stan
Immunogen: Extracellular domain, aa 539-1219.
Alternate Gene Names: CG11895, fmi, flam
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8-U1
Epitope Location or Sequence: Extracellular domain, aa 539-1219 (see depositor notes).
Uniprot ID: Q9V5N8 
Immunogen Sequence: Partial protein
Entrez Gene ID: 36125 
Additional Characterization:  
Antibody Registry ID: AB_2619583 
Additional Information: Flamingo is the protocadherin-like wing polarity protein stan.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Flamingo #74 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product Flamingo #74)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    The atypical cadherin Flamingo is required for sensory axon advance beyond intermediate target cells.
    Whitington PM
    Developmental biology 327.2 (2009 Mar 15): 447-57.

    Cohesin controls planar cell polarity by regulating the level of the seven-pass transmembrane cadherin Flamingo.
    Uemura T
    Genes to cells : devoted to molecular & cellular mechanisms 17.6 (2012 Jun): 509-24.

    A universal analysis tool for the detection of asymmetric signal distribution in microscopic images.
    Galic M
    Developmental dynamics : an official publication of the American Association of Anatomists 241.8 (2012 Aug): 1301-9.

    DWnt4 regulates the dorsoventral specificity of retinal projections in the Drosophila melanogaster visual system.
    Tabata T
    Nature neuroscience 9.1 (2006 Jan): 67-75.

    The cadherin Flamingo mediates level-dependent interactions that guide photoreceptor target choice in Drosophila.
    Clandinin TR
    Neuron 58.1 (2008 Apr 10): 26-33.

    Rabaptin-5 and Rabex-5 are neoplastic tumour suppressor genes that interact to modulate Rab5 dynamics in Drosophila melanogaster.
    Strutt D
    Developmental biology 385.1 (2014 Jan 1): 107-21.

    Temporal identity in axonal target layer recognition.
    Hummel T
    Nature 456.7223 (2008 Dec 11): 800-3.

    Distinct developmental mechanisms underlie the evolutionary diversification of Drosophila sex combs.
    Kopp A
    Proceedings of the National Academy of Sciences of the United States of America 106.12 (2009 Mar 24): 4764-9.

    The proteins encoded by the Drosophila Planar Polarity Effector genes inturned, fuzzy and fritz interact physically and can re-pattern the accumulation of "upstream" Planar Cell Polarity proteins.
    Adler PN
    Developmental biology 394.1 (2014 Oct 1): 156-69.

    Extensive nonmuscle expression and epithelial apicobasal localization of the Drosophila ALP/Enigma family protein, Zasp52.
    Stronach B
    Gene expression patterns : GEP 15.2 (2014 Jul): 67-79.

    IHC References
    All References

    The atypical cadherin Flamingo is required for sensory axon advance beyond intermediate target cells.
    Whitington PM
    Developmental biology 327.2 (2009 Mar 15): 447-57.

    Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix.
    Mlodzik M
    Open biology 9.8 (2019 Aug 30): 190148.

    Cohesin controls planar cell polarity by regulating the level of the seven-pass transmembrane cadherin Flamingo.
    Uemura T
    Genes to cells : devoted to molecular & cellular mechanisms 17.6 (2012 Jun): 509-24.

    A universal analysis tool for the detection of asymmetric signal distribution in microscopic images.
    Galic M
    Developmental dynamics : an official publication of the American Association of Anatomists 241.8 (2012 Aug): 1301-9.

    DWnt4 regulates the dorsoventral specificity of retinal projections in the Drosophila melanogaster visual system.
    Tabata T
    Nature neuroscience 9.1 (2006 Jan): 67-75.

    The cadherin Flamingo mediates level-dependent interactions that guide photoreceptor target choice in Drosophila.
    Clandinin TR
    Neuron 58.1 (2008 Apr 10): 26-33.

    Rabaptin-5 and Rabex-5 are neoplastic tumour suppressor genes that interact to modulate Rab5 dynamics in Drosophila melanogaster.
    Strutt D
    Developmental biology 385.1 (2014 Jan 1): 107-21.

    Temporal identity in axonal target layer recognition.
    Hummel T
    Nature 456.7223 (2008 Dec 11): 800-3.

    Distinct developmental mechanisms underlie the evolutionary diversification of Drosophila sex combs.
    Kopp A
    Proceedings of the National Academy of Sciences of the United States of America 106.12 (2009 Mar 24): 4764-9.

    The proteins encoded by the Drosophila Planar Polarity Effector genes inturned, fuzzy and fritz interact physically and can re-pattern the accumulation of "upstream" Planar Cell Polarity proteins.
    Adler PN
    Developmental biology 394.1 (2014 Oct 1): 156-69.

    Extensive nonmuscle expression and epithelial apicobasal localization of the Drosophila ALP/Enigma family protein, Zasp52.
    Stronach B
    Gene expression patterns : GEP 15.2 (2014 Jul): 67-79.

    Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled.
    Uemura T
    Cell 98.5 (1999 Sep 3): 585-95.

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