Flamingo #74
Catalog Fields
Product Name/ID: Flamingo #74
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: stan
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619583
Uniprot ID: Q9V5N8
Entrez Gene ID: 36125
Clonality: Monoclonal
Immunogen: Extracellular domain, aa 539-1219.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3-X63-Ag8-U1
Epitope Mapped: Yes
Antigen Name: Flamingo (starry night)
Epitope Location or Sequence: Extracellular domain, aa 539-1219 (see depositor notes).
Alternate Antigen Name:
Deposit Date: 1/7/2004
Antigen Molecular Weight: Predicted: 394 kDa
Depositor: Uemura, T.
Antigen Sequence:
Depositor Institution: Kyoto Univ. Graduate Sch.of Biostudies
Antigen Species: Drosophila
Depositor Notes: Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Flamingo is the protocadherin-like wing polarity protein stan.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Flamingo #74 was deposited to the DSHB by Uemura, T. (DSHB Hybridoma Product Flamingo #74)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled.
Uemura T
Cell 98.5 (1999 Sep 3): 585-95.