M114
Catalog Fields
Antigen: Sex lethal protein (female specific)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila Melanogaster
Depositor: Schedl, P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple female isoforms; Predicted: 34-40 kDa; Apparent 35-42 kDa
Predicted Species Reactivity:
Gene: Sxl
Immunogen: Recombinant lacZ-Sxl (255 C-terminal residues of isoform MS3/D) fusion protein
Alternate Gene Names: CG43770, Sx1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: 255 C-terminal residues of isoform MS3/D
Uniprot ID: P19339
Immunogen Sequence: Partial protein
Entrez Gene ID: 3772180
Antibody Registry ID: AB_528463
Additional Information: Sxl is the master regulator of sexual development and dosage compensation. M114 recognizes Sxl female isoforms (it may not recognize C-terminus variants) and will not recognize the two short male-specific isoforms. By western blot M114 recognizes two faint bands of 33 and 35 kDa only in head and thorax. Whole mount staining of embryos is specific for female embryos.
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M114 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product M114)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster.
Schedl P
Genes & development 5.3 (1991 Mar): 403-15.