M114

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$40.00
SKU: M114
View product citations for antibody M114 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Sex lethal protein (female specific)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila Melanogaster
Depositor: Schedl, P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple female isoforms; Predicted: 34-40 kDa; Apparent 35-42 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Sxl
Immunogen: Recombinant lacZ-Sxl (255 C-terminal residues of isoform MS3/D) fusion protein
Alternate Gene Names: CG43770, Sx1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: 255 C-terminal residues of isoform MS3/D
Uniprot ID: P19339 
Immunogen Sequence: Partial protein
Entrez Gene ID: 3772180 
Additional Characterization:  
Antibody Registry ID: AB_528463 
Additional Information: Sxl is the master regulator of sexual development and dosage compensation. M114 recognizes Sxl female isoforms (it may not recognize C-terminus variants) and will not recognize the two short male-specific isoforms. By western blot M114 recognizes two faint bands of 33 and 35 kDa only in head and thorax. Whole mount staining of embryos is specific for female embryos.
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M114 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product M114)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References

    Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.
    Salz HK
    Proceedings of the National Academy of Sciences of the United States of America 109.24 (2012 Jun 12): 9465-70.

    The N-terminal domain of Sxl protein disrupts Sxl autoregulation in females and promotes female-specific splicing of tra in males.
    Schedl PD
    Development (Cambridge, England) 126.13 (1999 Jul): 2841-53.

    Sex-lethal interacts with splicing factors in vitro and in vivo.
    Schedl PD
    Molecular and cellular biology 16.9 (1996 Sep): 5036-47.

    PPS, a large multidomain protein, functions with sex-lethal to regulate alternative splicing in Drosophila.
    Salz HK
    PLoS genetics 6.3 (2010 Mar 5): e1000872.

    Epitope Map References
    All References

    Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster.
    Schedl P
    Genes & development 5.3 (1991 Mar): 403-15.

    The N-terminal domain of Sxl protein disrupts Sxl autoregulation in females and promotes female-specific splicing of tra in males.
    Schedl PD
    Development (Cambridge, England) 126.13 (1999 Jul): 2841-53.

    The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries.
    Buszczak M
    Developmental biology 417.1 (2016 Sep 1): 50-62.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    Evidence for chromatin-remodeling complex PBAP-controlled maintenance of the Drosophila ovarian germline stem cells.
    Li M
    PloS one 9.7 (2014): e103473.

    Wolbachia-mediated male killing is associated with defective chromatin remodeling.
    Callaini G
    PloS one 7.1 (2012): e30045.

    A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
    Stathopoulos A
    Developmental cell 47.6 (2018 Dec 17): 773-784.e6.

    Drosophila female germline stem cells undergo mitosis without nuclear breakdown.
    Geyer PK
    Current biology : CB . (2021 Feb 1): .

    Sex-lethal interacts with splicing factors in vitro and in vivo.
    Schedl PD
    Molecular and cellular biology 16.9 (1996 Sep): 5036-47.

    PPS, a large multidomain protein, functions with sex-lethal to regulate alternative splicing in Drosophila.
    Salz HK
    PLoS genetics 6.3 (2010 Mar 5): e1000872.

    Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.
    Salz HK
    Proceedings of the National Academy of Sciences of the United States of America 109.24 (2012 Jun 12): 9465-70.

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