M18

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$40.00
SKU: M18
View product citations for antibody M18 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Sex-lethal protein, female-specific isoform
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schedl, P.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 35-38 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Sxl
Immunogen: LacZ-Sxl fusion protein
Alternate Gene Names: Sx1, CG43770
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P19339 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 3772180 
Additional Characterization:  
Antibody Registry ID: AB_528464 
Additional Information: RRID:AB_528464 FBgn0264270
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M18 was deposited to the DSHB by Schedl, P. (DSHB Hybridoma Product M18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

23 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Development of the male germline stem cell niche in Drosophila.
    Van Doren M
    Developmental biology 294.1 (2006 Jun 1): 92-103.

    beta-Spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS.
    Bashaw GJ
    Development (Cambridge, England) 134.2 (2007 Jan): 273-84.

    The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway.
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Independent functions of Slit-Robo repulsion and Netrin-Frazzled attraction regulate axon crossing at the midline in Drosophila.
    Bashaw GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.13 (2007 Mar 28): 3584-92.

    Cytoplasmic domain requirements for Frazzled-mediated attractive axon turning at the Drosophila midline.
    Bashaw GJ
    Development (Cambridge, England) 134.24 (2007 Dec): 4325-34.

    Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Wingless signaling initiates mitosis of primordial germ cells during development in Drosophila.
    Niki Y
    Mechanisms of development 125.5-6 (2008 May-Jun): 498-507.

    Protein phosphatase 2A negatively regulates aPKC signaling by modulating phosphorylation of Par-6 in Drosophila neuroblast asymmetric divisions.
    Matsuzaki F
    Journal of cell science 122.Pt 18 (2009 Sep 15): 3242-9.

    Jak-STAT regulation of male germline stem cell establishment during Drosophila embryogenesis.
    Wawersik M
    Developmental biology 334.2 (2009 Oct 15): 335-44.

    Notch and Egfr signaling act antagonistically to regulate germ-line stem cell niche formation in Drosophila male embryonic gonads.
    Kobayashi S
    Proceedings of the National Academy of Sciences of the United States of America 107.32 (2010 Aug 10): 14241-6.

    Apical deficiency triggers JNK-dependent apoptosis in the embryonic epidermis of Drosophila.
    Vincent JP
    Development (Cambridge, England) 138.14 (2011 Jul): 3021-31.

    Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.
    Salz HK
    Proceedings of the National Academy of Sciences of the United States of America 109.24 (2012 Jun 12): 9465-70.

    Jak-STAT regulation of cyst stem cell development in the Drosophila testis.
    Wawersik M
    Developmental biology 372.1 (2012 Dec 1): 5-16.

    Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development.
    Wu CT
    PLoS genetics 9.12 (2013): e1004013.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    Cdc42 and formin activity control non-muscle myosin dynamics during Drosophila heart morphogenesis.
    Bodmer R
    The Journal of cell biology 206.7 (2014 Sep 29): 909-22.

    Gene Dosage Imbalance Contributes to Chromosomal Instability-Induced Tumorigenesis.
    Milán M
    Developmental cell 36.3 (2016 Feb 8): 290-302.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    The m<sup>6</sup>A pathway facilitates sex determination in Drosophila.
    Lai EC
    Nature communications 8. (2017 Jul 4): 15737.

    A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
    Stathopoulos A
    Developmental cell 47.6 (2018 Dec 17): 773-784.e6.

    WB References
    IHC References
    IP References
    All References

    Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster.
    Schedl P
    Genes & development 5.3 (1991 Mar): 403-15.

    Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Development of the male germline stem cell niche in Drosophila.
    Van Doren M
    Developmental biology 294.1 (2006 Jun 1): 92-103.

    beta-Spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS.
    Bashaw GJ
    Development (Cambridge, England) 134.2 (2007 Jan): 273-84.

    The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway.
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Independent functions of Slit-Robo repulsion and Netrin-Frazzled attraction regulate axon crossing at the midline in Drosophila.
    Bashaw GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.13 (2007 Mar 28): 3584-92.

    Cytoplasmic domain requirements for Frazzled-mediated attractive axon turning at the Drosophila midline.
    Bashaw GJ
    Development (Cambridge, England) 134.24 (2007 Dec): 4325-34.

    Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Wingless signaling initiates mitosis of primordial germ cells during development in Drosophila.
    Niki Y
    Mechanisms of development 125.5-6 (2008 May-Jun): 498-507.

    Protein phosphatase 2A negatively regulates aPKC signaling by modulating phosphorylation of Par-6 in Drosophila neuroblast asymmetric divisions.
    Matsuzaki F
    Journal of cell science 122.Pt 18 (2009 Sep 15): 3242-9.

    Jak-STAT regulation of male germline stem cell establishment during Drosophila embryogenesis.
    Wawersik M
    Developmental biology 334.2 (2009 Oct 15): 335-44.

    Notch and Egfr signaling act antagonistically to regulate germ-line stem cell niche formation in Drosophila male embryonic gonads.
    Kobayashi S
    Proceedings of the National Academy of Sciences of the United States of America 107.32 (2010 Aug 10): 14241-6.

    Apical deficiency triggers JNK-dependent apoptosis in the embryonic epidermis of Drosophila.
    Vincent JP
    Development (Cambridge, England) 138.14 (2011 Jul): 3021-31.

    Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.
    Salz HK
    Proceedings of the National Academy of Sciences of the United States of America 109.24 (2012 Jun 12): 9465-70.

    Jak-STAT regulation of cyst stem cell development in the Drosophila testis.
    Wawersik M
    Developmental biology 372.1 (2012 Dec 1): 5-16.

    Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development.
    Wu CT
    PLoS genetics 9.12 (2013): e1004013.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    Cdc42 and formin activity control non-muscle myosin dynamics during Drosophila heart morphogenesis.
    Bodmer R
    The Journal of cell biology 206.7 (2014 Sep 29): 909-22.

    Gene Dosage Imbalance Contributes to Chromosomal Instability-Induced Tumorigenesis.
    Milán M
    Developmental cell 36.3 (2016 Feb 8): 290-302.

    The sexual identity of adult intestinal stem cells controls organ size and plasticity.
    Miguel-Aliaga I
    Nature 530.7590 (2016 Feb 18): 344-8.

    The m<sup>6</sup>A pathway facilitates sex determination in Drosophila.
    Lai EC
    Nature communications 8. (2017 Jul 4): 15737.

    A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
    Stathopoulos A
    Developmental cell 47.6 (2018 Dec 17): 773-784.e6.

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