Rip
Catalog Fields
Clone ID/Product Name: Rip
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Cnp
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 25275
Clonality: Monoclonal
Immunogen: Olfactory bulb granule cell layer from P [postnatal day] 12 rats and white matter from adult rats homogenized in phosphate-buffered saline.
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: CNP / Rip antigen
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/7/1996
Antigen Molecular Weight: Apparent: 23 and 160 kDa
Depositor: Hockfield, S.
Antigen Sequence:
Depositor Institution: Yale University School of Medicine
Antigen Species: Rat
Depositor Notes: Rip stains oligodendrocytes from early in their differentiation into adulthood. Expression preceeds myelin proteins. Rip stains mature oligodendrocytes, their cytoplasmic processes, and myelin in the adult rat spinal cord and cerebellum
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Hamster, Mouse, Rat
Additional Information: Rip antigen has been identified as 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP). See PMID: 16786579.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rip was deposited to the DSHB by Hockfield, S. (DSHB Hybridoma Product Rip)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
In situ demonstration of mature oligodendrocytes and their processes: an immunocytochemical study with a new monoclonal antibody, rip.
Waxman SG
Glia 2.5 (1989): 380-90.