Rip

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$40.00
SKU: Rip
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DSHB Data Sheet

Catalog Fields

Antigen: CNP / Rip antigen
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Hockfield, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University School of Medicine
Positive Tested Species Reactivity: Hamster, Mouse, Rat
Depositors Notes: Rip stains oligodendrocytes from early in their differentiation into adulthood. Expression preceeds myelin proteins. Rip stains mature oligodendrocytes, their cytoplasmic processes, and myelin in the adult rat spinal cord and cerebellum
Antigen Molecular Weight: Apparent: 23 and 160 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Cnp
Immunogen: Olfactory bulb granule cell layer from P [postnatal day] 12 rats and white matter from adult rats homogenized in phosphate-buffered saline.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P13233 
Immunogen Sequence:
Entrez Gene ID: 25275 
Additional Characterization:  
Antibody Registry ID: AB_531796 
Additional Information: Rip antigen has been identified as 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP). See PMID: 16786579.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rip was deposited to the DSHB by Hockfield, S. (DSHB Hybridoma Product Rip)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    In situ demonstration of mature oligodendrocytes and their processes: an immunocytochemical study with a new monoclonal antibody, rip.
    Waxman SG
    Glia 2.5 (1989): 380-90.

    Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations.
    Magnuson DS
    Experimental cell research 252.1 (1999 Oct 10): 75-95.

    Astroglia induce neurogenesis from adult neural stem cells.
    Gage FH
    Nature 417.6884 (2002 May 2): 39-44.

    Cellular localization of TRPC3 channel in rat brain: preferential distribution to oligodendrocytes.
    Bernardi G
    Neuroscience letters 365.2 (2004 Jul 22): 137-42.

    Exogenous leukemia inhibitory factor stimulates oligodendrocyte progenitor cell proliferation and enhances hippocampal remyelination.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2100-9.

    Investigating tonic Wnt signaling throughout the adult CNS and in the hippocampal neurogenic niche of BatGal and ins-TopGal mice.
    Ring RH
    Cellular and molecular neurobiology 32.7 (2012 Oct): 1159-74.

    Efficient cultivation of neural stem cells with controlled delivery of FGF-2.
    Melone MA
    Stem cell research 10.1 (2013 Jan): 85-94.

    Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
    DiCicco-Bloom E
    Proceedings of the National Academy of Sciences of the United States of America 98.8 (2001 Apr 10): 4758-63.

    Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System.
    Schaffer DV
    Stem cell reports 8.6 (2017 Jun 6): 1770-1783.

    Monoclonal antibody Rip specifically recognizes 2',3'-cyclic nucleotide 3'-phosphodiesterase in oligodendrocytes.
    Itoh K
    Journal of neuroscience research 84.3 (2006 Aug 15): 525-33.

    Glioma-derived exosomes drive the differentiation of neural stem cells to astrocytes.
    Xie JY
    PloS one 15.7 (2020): e0234614.

    WB References
    IHC References

    In situ demonstration of mature oligodendrocytes and their processes: an immunocytochemical study with a new monoclonal antibody, rip.
    Waxman SG
    Glia 2.5 (1989): 380-90.

    Oligodendrocytes and myelin formation along the optic tract of the developing hamster: an immunohistochemical study using the Rip antibody.
    Schneider GE
    Glia 6.2 (1992): 138-48.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    IP References
    All References

    In situ demonstration of mature oligodendrocytes and their processes: an immunocytochemical study with a new monoclonal antibody, rip.
    Waxman SG
    Glia 2.5 (1989): 380-90.

    Oligodendrocytes and myelin formation along the optic tract of the developing hamster: an immunohistochemical study using the Rip antibody.
    Schneider GE
    Glia 6.2 (1992): 138-48.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations.
    Magnuson DS
    Experimental cell research 252.1 (1999 Oct 10): 75-95.

    Astroglia induce neurogenesis from adult neural stem cells.
    Gage FH
    Nature 417.6884 (2002 May 2): 39-44.

    Cellular localization of TRPC3 channel in rat brain: preferential distribution to oligodendrocytes.
    Bernardi G
    Neuroscience letters 365.2 (2004 Jul 22): 137-42.

    Exogenous leukemia inhibitory factor stimulates oligodendrocyte progenitor cell proliferation and enhances hippocampal remyelination.
    Patterson PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2100-9.

    Investigating tonic Wnt signaling throughout the adult CNS and in the hippocampal neurogenic niche of BatGal and ins-TopGal mice.
    Ring RH
    Cellular and molecular neurobiology 32.7 (2012 Oct): 1159-74.

    Efficient cultivation of neural stem cells with controlled delivery of FGF-2.
    Melone MA
    Stem cell research 10.1 (2013 Jan): 85-94.

    Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
    DiCicco-Bloom E
    Proceedings of the National Academy of Sciences of the United States of America 98.8 (2001 Apr 10): 4758-63.

    Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System.
    Schaffer DV
    Stem cell reports 8.6 (2017 Jun 6): 1770-1783.

    Monoclonal antibody Rip specifically recognizes 2',3'-cyclic nucleotide 3'-phosphodiesterase in oligodendrocytes.
    Itoh K
    Journal of neuroscience research 84.3 (2006 Aug 15): 525-33.

    Glioma-derived exosomes drive the differentiation of neural stem cells to astrocytes.
    Xie JY
    PloS one 15.7 (2020): e0234614.

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