Tango
Catalog Fields
Antigen: Tango
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Crews, S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of North Carolina at Chapel Hill
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Strong reduction in staining in mutant embryos.
Predicted Species Reactivity:
Gene: tgo
Immunogen: Recombinant Tgo (aa 1-393)/Gst fusion protein.
Alternate Gene Names: CG11987, dARNT, tango, bHLHe4, HIF-1beta
Alternate Antibody Name: mAb-Tgo
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63 Ag8 653
Epitope Location or Sequence: aa 1-393
Uniprot ID: O15945
Immunogen Sequence: Partial protein
Entrez Gene ID: 41084
Antibody Registry ID: AB_528486
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Tango was deposited to the DSHB by Crews, S. (DSHB Hybridoma Product Tango)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
The Drosophila tango gene encodes a bHLH-PAS protein that is orthologous to mammalian Arnt and controls CNS midline and tracheal development.
Crews S
Development (Cambridge, England) 124.22 (1997 Nov): 4571-82.