Antigen: GASP (Gene Analogous to Small Peritrophins)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Krasnow, M.A.
Host Species: mouse
Depositors Institution: Stanford University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 2A12 stains from stages 14/15 to 17. Fix the embryos about 20-30 minutes in fix solution described in the initial publication .
Antigen Molecular Weight: Predicted: 24 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Location or Sequence:
Uniprot ID: O97377
Entrez Gene ID: 40745
Antibody Registry ID: AB_528492
Additional Information: The antigen protein recognized by 2A12 was identified as GASP in 2013 [PMID: 23826295].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2A12 was deposited to the DSHB by Krasnow, M.A. (DSHB Hybridoma Product 2A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).