2A12

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 2A12
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: GASP
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_528492 
Uniprot ID: O97377 
RRID:  
Entrez Gene ID: 40745 
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: GASP (Gene Analogous to Small Peritrophins)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/28/1995
Antigen Molecular Weight: Predicted: 24 kDa
Depositor: Krasnow, M.A.
Antigen Sequence:
Depositor Institution: Stanford University
Antigen Species: Drosophila
Depositor Notes: 2A12 stains from stages 14/15 to 17. Fix the embryos about 20-30 minutes in fix solution described in the initial publication [7707967].
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The antigen protein recognized by 2A12 was identified as GASP in 2013 [PMID: 23826295].
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2A12 was deposited to the DSHB by Krasnow, M.A. (DSHB Hybridoma Product 2A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

40 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes.
    Patel NH
    Methods in cell biology 44. (1994): 445-87.

    Three-dimensional folding and functional organization principles of the Drosophila genome.
    Cavalli G
    Cell 148.3 (2012 Feb 3): 458-72.

    Development of the Drosophila tracheal system occurs by a series of morphologically distinct but genetically coupled branching events.
    Krasnow MA
    Development (Cambridge, England) 122.5 (1996 May): 1395-407.

    Hedgehog is a positive regulator of FGF signalling during embryonic tracheal cell migration.
    Araújo SJ
    PloS one 9.3 (2014): e92682.

    Specific tracheal migration is mediated by complementary expression of cell surface proteins.
    Casanova J
    Genes & development 15.12 (2001 Jun 15): 1554-62.

    Control of tracheal tubulogenesis by Wingless signaling.
    Hayashi S
    Development (Cambridge, England) 127.20 (2000 Oct): 4433-42.

    Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea.
    Hayashi S
    Development (Cambridge, England) 126.20 (1999 Oct): 4455-63.

    Drosophila dopamine synthesis pathway genes regulate tracheal morphogenesis.
    O'Donnell JM
    Developmental biology 308.1 (2007 Aug 1): 30-43.

    Sequoia establishes tip-cell number in Drosophila trachea by regulating FGF levels.
    Casanova J
    Journal of cell science 124.Pt 14 (2011 Jul 15): 2335-40.

    Social interactions among epithelial cells during tracheal branching morphogenesis.
    Krasnow MA
    Nature 441.7094 (2006 Jun 8): 746-9.

    Gal4 in the Drosophila female germline.
    Rørth P
    Mechanisms of development 78.1-2 (1998 Nov): 113-8.

    A myocardin-related transcription factor regulates activity of serum response factor in Drosophila.
    Olson EN
    Proceedings of the National Academy of Sciences of the United States of America 101.34 (2004 Aug 24): 12567-72.

    The jing Zn-finger transcription factor is a mediator of cellular differentiation in the Drosophila CNS midline and trachea.
    Sonnenfeld MJ
    Development (Cambridge, England) 129.11 (2002 Jun): 2591-606.

    TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms.
    Schuh R
    BioTechniques 44.6 (2008 May): 765-72.

    Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size.
    Beitel GJ
    Nature cell biology 14.5 (2012 Mar 25): 518-25.

    Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila.
    Samakovlis C
    Nature cell biology 12.11 (2010 Nov): 1071-7.

    Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface.
    Vorbrüggen G
    Proceedings of the National Academy of Sciences of the United States of America 106.29 (2009 Jul 21): 11984-8.

    The Drosophila Extradenticle and Homothorax selector proteins control branchless/FGF expression in mesodermal bridge-cells.
    Affolter M
    EMBO reports 6.8 (2005 Aug): 762-8.

    Control of airway tube diameter and integrity by secreted chitin-binding proteins in Drosophila.
    Samakovlis C
    PloS one 8.6 (2013): e67415.

    An Org-1-Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila.
    Vincent A
    Development (Cambridge, England) 141.19 (2014 Oct): 3761-71.

    Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila.
    Samakovlis C
    Developmental cell 13.2 (2007 Aug): 214-25.

    The secreted AdamTS-A metalloprotease is required for collective cell migration.
    Andrew DJ
    Development (Cambridge, England) 140.9 (2013 May): 1981-93.

    O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions.
    Okajima T
    Nature communications 2. (2011 Dec 13): 583.

    Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components.
    Uv AE
    Development (Cambridge, England) 137.14 (2010 Jul): 2427-37.

    The claudin Megatrachea protein complex.
    Schuh R
    The Journal of biological chemistry 287.44 (2012 Oct 26): 36756-65.

    Echinoid regulates tracheal morphology and fusion cell fate in Drosophila.
    Nilson LA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.9 (2010 Sep): 2509-19.

    Tissue-autonomous EcR functions are required for concurrent organ morphogenesis in the Drosophila embryo.
    Uv A
    Mechanisms of development 127.5-6 (2010 May-Jun): 308-19.

    COPI vesicle transport is a common requirement for tube expansion in Drosophila.
    Samakovlis C
    PloS one 3.4 (2008 Apr 9): e1964.

    Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila trachea.
    Samakovlis C
    Current biology : CB 16.2 (2006 Jan 24): 180-5.

    Non-canonical roles for Yorkie and Drosophila Inhibitor of Apoptosis 1 in epithelial tube size control.
    Beitel GJ
    PloS one 9.7 (2014): e101609.

    Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous.
    Shilo BZ
    Developmental cell 16.6 (2009 Jun): 877-88.

    The Drosophila tango gene encodes a bHLH-PAS protein that is orthologous to mammalian Arnt and controls CNS midline and tracheal development.
    Crews S
    Development (Cambridge, England) 124.22 (1997 Nov): 4571-82.

    Specificity of FGF signaling in cell migration in Drosophila.
    Affolter M
    Development (Cambridge, England) 128.22 (2001 Nov): 4563-72.

    All References

    Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes.
    Patel NH
    Methods in cell biology 44. (1994): 445-87.

    Three-dimensional folding and functional organization principles of the Drosophila genome.
    Cavalli G
    Cell 148.3 (2012 Feb 3): 458-72.

    Development of the Drosophila tracheal system occurs by a series of morphologically distinct but genetically coupled branching events.
    Krasnow MA
    Development (Cambridge, England) 122.5 (1996 May): 1395-407.

    Hedgehog is a positive regulator of FGF signalling during embryonic tracheal cell migration.
    Araújo SJ
    PloS one 9.3 (2014): e92682.

    Specific tracheal migration is mediated by complementary expression of cell surface proteins.
    Casanova J
    Genes & development 15.12 (2001 Jun 15): 1554-62.

    Control of tracheal tubulogenesis by Wingless signaling.
    Hayashi S
    Development (Cambridge, England) 127.20 (2000 Oct): 4433-42.

    Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea.
    Hayashi S
    Development (Cambridge, England) 126.20 (1999 Oct): 4455-63.

    Drosophila dopamine synthesis pathway genes regulate tracheal morphogenesis.
    O'Donnell JM
    Developmental biology 308.1 (2007 Aug 1): 30-43.

    Sequoia establishes tip-cell number in Drosophila trachea by regulating FGF levels.
    Casanova J
    Journal of cell science 124.Pt 14 (2011 Jul 15): 2335-40.

    Social interactions among epithelial cells during tracheal branching morphogenesis.
    Krasnow MA
    Nature 441.7094 (2006 Jun 8): 746-9.

    Gal4 in the Drosophila female germline.
    Rørth P
    Mechanisms of development 78.1-2 (1998 Nov): 113-8.

    A myocardin-related transcription factor regulates activity of serum response factor in Drosophila.
    Olson EN
    Proceedings of the National Academy of Sciences of the United States of America 101.34 (2004 Aug 24): 12567-72.

    The jing Zn-finger transcription factor is a mediator of cellular differentiation in the Drosophila CNS midline and trachea.
    Sonnenfeld MJ
    Development (Cambridge, England) 129.11 (2002 Jun): 2591-606.

    TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms.
    Schuh R
    BioTechniques 44.6 (2008 May): 765-72.

    Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size.
    Beitel GJ
    Nature cell biology 14.5 (2012 Mar 25): 518-25.

    Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila.
    Samakovlis C
    Nature cell biology 12.11 (2010 Nov): 1071-7.

    Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface.
    Vorbrüggen G
    Proceedings of the National Academy of Sciences of the United States of America 106.29 (2009 Jul 21): 11984-8.

    The Drosophila Extradenticle and Homothorax selector proteins control branchless/FGF expression in mesodermal bridge-cells.
    Affolter M
    EMBO reports 6.8 (2005 Aug): 762-8.

    Control of airway tube diameter and integrity by secreted chitin-binding proteins in Drosophila.
    Samakovlis C
    PloS one 8.6 (2013): e67415.

    An Org-1-Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila.
    Vincent A
    Development (Cambridge, England) 141.19 (2014 Oct): 3761-71.

    Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila.
    Samakovlis C
    Developmental cell 13.2 (2007 Aug): 214-25.

    The secreted AdamTS-A metalloprotease is required for collective cell migration.
    Andrew DJ
    Development (Cambridge, England) 140.9 (2013 May): 1981-93.

    O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions.
    Okajima T
    Nature communications 2. (2011 Dec 13): 583.

    Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components.
    Uv AE
    Development (Cambridge, England) 137.14 (2010 Jul): 2427-37.

    The claudin Megatrachea protein complex.
    Schuh R
    The Journal of biological chemistry 287.44 (2012 Oct 26): 36756-65.

    Echinoid regulates tracheal morphology and fusion cell fate in Drosophila.
    Nilson LA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.9 (2010 Sep): 2509-19.

    Tissue-autonomous EcR functions are required for concurrent organ morphogenesis in the Drosophila embryo.
    Uv A
    Mechanisms of development 127.5-6 (2010 May-Jun): 308-19.

    COPI vesicle transport is a common requirement for tube expansion in Drosophila.
    Samakovlis C
    PloS one 3.4 (2008 Apr 9): e1964.

    Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila trachea.
    Samakovlis C
    Current biology : CB 16.2 (2006 Jan 24): 180-5.

    Non-canonical roles for Yorkie and Drosophila Inhibitor of Apoptosis 1 in epithelial tube size control.
    Beitel GJ
    PloS one 9.7 (2014): e101609.

    Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous.
    Shilo BZ
    Developmental cell 16.6 (2009 Jun): 877-88.

    The Drosophila tango gene encodes a bHLH-PAS protein that is orthologous to mammalian Arnt and controls CNS midline and tracheal development.
    Crews S
    Development (Cambridge, England) 124.22 (1997 Nov): 4571-82.

    Specificity of FGF signaling in cell migration in Drosophila.
    Affolter M
    Development (Cambridge, England) 128.22 (2001 Nov): 4563-72.

    Systematic expression and loss-of-function analysis defines spatially restricted requirements for Drosophila RhoGEFs and RhoGAPs in leg morphogenesis.
    Hatini V
    Mechanisms of development 128.1-2 (2011 Jan-Feb): 5-17.

    The Drosophila jing gene is a downstream target in the Trachealess/Tango tracheal pathway.
    Sonnenfeld M
    Development genes and evolution 220.7-8 (2010 Dec): 191-206.

    Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control.
    Hayashi S
    Nature communications 4. (2013): 1358.

    Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape.
    Hayashi S
    Cell reports 7.4 (2014 May 22): 941-50.

    The novel Smad protein Expansion regulates the receptor tyrosine kinase pathway to control Drosophila tracheal tube size.
    Jiang L
    Developmental biology 393.1 (2014 Sep 1): 93-108.

    rebuff regulates apical luminal matrix to control tube size in Drosophila trachea.
    Jiang L
    Biology open 7.9 (2018 Sep 5): .

    Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core.
    van der Wel PCA
    Nature communications 8. (2017 May 24): 15462.

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