Vasa 46F11

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SKU: Vasa 46F11
View product citations for antibody Vasa 46F11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Vasa ATP-dependent RNA helicase, isoform A
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Jan, Y.-N.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California San Francisco
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Mab46F11 was generated using horseradish peroxidase as the immunogen. Mab46F11 does not recognize HRP and the relationship of HRP to the Vasa antigen is not known. The antigen is associated with polar granules and nuclear bodies during Drosophila embryogenesis.
Antigen Molecular Weight: 72.3 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: vasa
Immunogen: Horseradish peroxidase (see Depositor's notes)
Alternate Gene Names: Dmel_CG46283
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P09052 
Immunogen Sequence: Full length
Entrez Gene ID: 26067080 
Additional Characterization:  
Antibody Registry ID: AB_10571464 
Additional Information: RRID:AB_10571464
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Vasa 46F11 was deposited to the DSHB by Jan, Y.-N. (DSHB Hybridoma Product Vasa 46F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Identification of a component of Drosophila polar granules.
    Jan YN
    Development (Cambridge, England) 103.4 (1988 Aug): 625-40.

    IF References
    WB References
    IHC References
    All References

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