mAB 30 (asv30)

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$40.00
SKU: mAB 30 (asv30)
View product citations for antibody mAB 30 (asv30) on CiteAb

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Available: 74

DSHB Data Sheet

Catalog Fields

Antigen: synaptotagmin-1, cytoplasmic domain
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Reichardt, L.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Physiology, University of California
Positive Tested Species Reactivity: Chicken, Fish, Human, Mouse, Rat, Xenopus
Depositors Notes: mAB 30 (asv30) as a lower affinity than mAb 48 (asv 48). The two antibodies recognize the same antigen, Syt1. mAB 30 (asv30) is detergent sensitive: TritonX-100 sensitive epitope (reversible), epitope present in presence of CHAPS.
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 65 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Syt1
Immunogen: Synaptic junctional complex proteins prepared from rat brain synaptic membranes
Alternate Gene Names: P65
Alternate Antibody Name: SV 30
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Cytoplasmic (aa 80-421)
Uniprot ID: P21707 
Immunogen Sequence: Full length protein
Entrez Gene ID: 25716 
Additional Characterization:  
Antibody Registry ID: AB_2295002 
Additional Information: *Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing dshb@uiowa.edu or by calling 319 335-3826.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 30 (asv30) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAB 30 (asv30))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
    Reichardt LF
    The Journal of cell biology 91.1 (1981 Oct): 257-69.

    Transmembrane protein 108 is required for glutamatergic transmission in dentate gyrus.
    Mei L
    Proceedings of the National Academy of Sciences of the United States of America 114.5 (2017 Jan 31): 1177-1182.

    IHC References

    Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
    Reichardt LF
    The Journal of cell biology 91.1 (1981 Oct): 257-69.

    Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 2.7 (1982 Jul): 843-52.

    IP References
    All References

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