mAB 30 (asv30)

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SKU: mAB 30 (asv30)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: mAB 30 (asv30)
Available to For-Profits: No
Alternate Antibody Name: SV 30
Gene Name: Syt1
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2295002 
Uniprot ID: P21707 
Entrez Gene ID: 25716 
Clonality: Monoclonal
Immunogen: Synaptic junctional complex proteins prepared from rat brain synaptic membranes
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: synaptotagmin-1, cytoplasmic domain
Epitope Location or Sequence: Cytoplasmic (aa 80-421)
Alternate Antigen Name:
Deposit Date: 4/20/1999
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 65 kDa
Depositor: Reichardt, L.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: rat
Depositor Notes: mAB 30 (asv30) as a lower affinity than mAb 48 (asv 48). The two antibodies recognize the same antigen, Syt1. mAB 30 (asv30) is detergent sensitive: TritonX-100 sensitive epitope (reversible), epitope present in presence of CHAPS.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Human, Mouse, Rat, Xenopus
Additional Information: *Not available to for-profit organizations. For further information please contact the Office of Technology Management and Advancement at the University of California. Refer to case number 95-082. Email : Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing or by calling 319 335-3826.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 30 (asv30) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAB 30 (asv30))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
    Reichardt LF
    The Journal of cell biology 91.1 (1981 Oct): 257-69.

    Transmembrane protein 108 is required for glutamatergic transmission in dentate gyrus.
    Mei L
    Proceedings of the National Academy of Sciences of the United States of America 114.5 (2017 Jan 31): 1177-1182.

    IHC References

    Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
    Reichardt LF
    The Journal of cell biology 91.1 (1981 Oct): 257-69.

    Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 2.7 (1982 Jul): 843-52.

    IP References
    All References

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