mAb 48 (asv 48)

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SKU: mAb 48 (asv 48)
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DSHB Data Sheet

Catalog Fields

Antigen: synaptotagmin-1, cytoplasmic domain
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Reichardt, L.
Isotype: MIgG2b, kappa light chain
Depositors Institution: Department of Physiology, University of California
Host Species: mouse
Depositors Notes: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing or by calling 319 335-3826.
Positive Tested Species Reactivity: Chicken, Fish, Human, Lamprey, Mouse, Rat, Xenopus, Zebrafish
Gene: Syt1
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 65 kDa
Alternate Gene Names: P65
Immunogen: Synaptic junctional complex proteins prepared from rat brain synaptic membranes
Clonality: Monoclonal
Alternate Antibody Name: SV 48
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Cytoplasmic (aa 80-421)
Uniprot ID: P21707 
Immunogen Sequence: Full length protein
Entrez Gene ID: 25716 
Additional Information: This antibody was validated for tissue fixed with 4% paraformaldehyde and cryosections following OCT embedding. Detergent sensitive / Triton X-100. This antibody recognizes synaptotagmin 1 and does not cross react with synaptotagmin 2. See Fox and Sanes (2007, PUDMED 17492637). mAb 48 (asv 48) has a higher affinity than mAb 30 (asv30). The two antibodies recognize the same antigen, Syt1.
Antibody Registry ID: AB_2199314 
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 48 (asv 48) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 48 (asv 48))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References

    Synaptotagmin I and II are present in distinct subsets of central synapses.
    Sanes JR
    The Journal of comparative neurology 503.2 (2007 Jul 10): 280-96.

    All References

    Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
    Reichardt LF
    The Journal of cell biology 91.1 (1981 Oct): 257-69.

    Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion.
    Reichardt LF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 2.7 (1982 Jul): 843-52.

    Synaptotagmins I and II in the developing rat auditory brainstem: Synaptotagmin I is transiently expressed in glutamate-releasing immature inhibitory terminals.
    Gillespie DC
    The Journal of comparative neurology 519.12 (2011 Aug 15): 2417-33.

    Synaptotagmin I and II are present in distinct subsets of central synapses.
    Sanes JR
    The Journal of comparative neurology 503.2 (2007 Jul 10): 280-96.

    Cyclic AMP promotes axon regeneration, lesion repair and neuronal survival in lampreys after spinal cord injury.
    Morgan JR
    Experimental neurology 250. (2013 Dec): 31-42.

    Reducing synuclein accumulation improves neuronal survival after spinal cord injury.
    Morgan JR
    Experimental neurology 278. (2016 Apr): 105-15.

    Neuroproteomics in the auditory brainstem: candidate proteins for ultrafast and precise information processing.
    Friauf E
    Molecular and cellular neurosciences 64. (2015 Jan): 9-23.

    Early presymptomatic cholinergic dysfunction in a murine model of amyotrophic lateral sclerosis.
    Navarro X
    Brain and behavior 3.2 (2013 Mar): 145-58.

    Brain region-dependent differential expression of alpha-synuclein.
    Tanaka M
    The Journal of comparative neurology 524.6 (2016 Apr 15): 1236-58.

    Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain.
    Purcell AW
    Proteomics 14.2-3 (2014 Feb): 241-61.

    Mesenchymal Stem Cells Therapy Improved the Streptozotocin-Induced Behavioral and Hippocampal Impairment in Rats.
    Reggiani PC
    Molecular neurobiology . (2019 Aug 10): .

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