mAb 48 (asv 48)
Catalog Fields
Product Name/ID: mAb 48 (asv 48)
Available to For-Profits: No
Alternate Antibody Name: SV 48
Gene Name: Syt1
Ab Isotype: MIgG2b, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2199314
Uniprot ID: P21707
Entrez Gene ID: 25716
Clonality: Monoclonal
Immunogen: Synaptic junctional complex proteins prepared from rat brain synaptic membranes
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: synaptotagmin-1, cytoplasmic domain
Epitope Location or Sequence: Cytoplasmic (aa 80-421)
Alternate Antigen Name:
Deposit Date: 4/20/1999
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 65 kDa
Depositor: Reichardt, L.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: rat
Depositor Notes: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing dshb@uiowa.edu or by calling 319 335-3826.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Human, Lamprey, Mouse, Rat, Xenopus, Zebrafish
Additional Information: *Not available to for-profit organizations. For further information please contact the Office of Technology Management and Advancement at the University of California. Refer to case number 95-082. Email : innovation@ucsf.edu. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing dshb@uiowa.edu or by calling 319 335-3826.
This antibody was validated for tissue fixed with 4% paraformaldehyde and cryosections following OCT embedding. Detergent sensitive / Triton X-100. This antibody recognizes synaptotagmin 1 and does not cross react with synaptotagmin 2. See Fox and Sanes (2007, PUDMED 17492637). mAb 48 (asv 48) has a higher affinity than mAb 30 (asv30). The two antibodies recognize the same antigen, Syt1.
This antibody was validated for tissue fixed with 4% paraformaldehyde and cryosections following OCT embedding. Detergent sensitive / Triton X-100. This antibody recognizes synaptotagmin 1 and does not cross react with synaptotagmin 2. See Fox and Sanes (2007, PUDMED 17492637). mAb 48 (asv 48) has a higher affinity than mAb 30 (asv30). The two antibodies recognize the same antigen, Syt1.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 48 (asv 48) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 48 (asv 48))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
Reichardt LF
The Journal of cell biology 91.1 (1981 Oct): 257-69.