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SKU: mAbdac1-1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: mAbdac1-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: dac
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_579773 
Uniprot ID: Q8IP19 
Entrez Gene ID: 34982 
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing aa 149-368 of Dachshund
Immunogen Sequence: Partial protein
Myeloma Strain: P3XAg8.653
Epitope Mapped: Yes
Antigen Name: Dachshund protein, Drosophila
Epitope Location or Sequence: aa 149-368
Alternate Antigen Name:
Deposit Date: 8/2/2006
Antigen Molecular Weight: Multiple isoforms; Predicted: between 112 and 114 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: The antibody was developed by Graeme Mardon in Dr. Rubin’s lab (contact: The antibody reproduces the pattern observed by in situ hybridization with a dac cDNA probe (unpublished observations) and an enhancer trap insert in dac.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAbdac1-1 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product mAbdac1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    dachshund encodes a nuclear protein required for normal eye and leg development in Drosophila.
    Rubin GM
    Development (Cambridge, England) 120.12 (1994 Dec): 3473-86.

    IF References
    IHC References
    Epitope Map References

    dachshund encodes a nuclear protein required for normal eye and leg development in Drosophila.
    Rubin GM
    Development (Cambridge, England) 120.12 (1994 Dec): 3473-86.

    All References

    dachshund encodes a nuclear protein required for normal eye and leg development in Drosophila.
    Rubin GM
    Development (Cambridge, England) 120.12 (1994 Dec): 3473-86.

    Sex-specific expression of a HOX gene associated with rapid morphological evolution.
    Kopp A
    Developmental biology 311.2 (2007 Nov 15): 277-86.

    Context-Dependent Tumorigenic Effect of Testis-Specific Mitochondrial Protein Tiny Tim 2 in Drosophila Somatic Epithelia.
    Gonzalez C
    Cells 9.8 (2020 Aug 6): .

    Regulation of glia number in Drosophila by Rap/Fzr, an activator of the anaphase-promoting complex, and Loco, an RGS protein.
    Venkatesh TR
    Genetics 178.4 (2008 Apr): 2003-16.

    Enteroendocrine cells are generated from stem cells through a distinct progenitor in the adult Drosophila posterior midgut.
    Hou SX
    Development (Cambridge, England) 142.4 (2015 Feb 15): 644-53.

    Hedgehog, transmitted along retinal axons, triggers neurogenesis in the developing visual centers of the Drosophila brain.
    Kunes S
    Cell 86.3 (1996 Aug 9): 411-22.

    A retinal axon fascicle uses spitz, an EGF receptor ligand, to construct a synaptic cartridge in the brain of Drosophila.
    Kunes S
    Cell 95.5 (1998 Nov 25): 693-703.

    The microcephaly protein Asp regulates neuroepithelium morphogenesis by controlling the spatial distribution of myosin II.
    Basto R
    Nature cell biology 15.11 (2013 Nov): 1294-306.

    Drosophila CORL is required for Smad2-mediated activation of Ecdysone Receptor expression in the mushroom body.
    Newfeld SJ
    Development (Cambridge, England) 139.18 (2012 Sep): 3392-401.

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