mAbdac1-1
Catalog Fields
Clone ID/Product Name: mAbdac1-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: dac
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Entrez Gene ID: 34982
Clonality: Monoclonal
Immunogen: Recombinant fusion protein containing aa 149-368 of Dachshund
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3XAg8.653
Epitope Mapped: Yes
Antigen Name: Dachshund protein, Drosophila
Epitope Location or Sequence: aa 149-368
Alternate Antigen Name:
Deposit Date: 8/2/2006
Antigen Molecular Weight: Multiple isoforms; Predicted: between 112 and 114 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: The antibody was developed by Graeme Mardon in Dr. Rubin’s lab (contact: gmardon@tmc.edu). The antibody reproduces the pattern observed by in situ hybridization with a dac cDNA probe (unpublished observations) and an enhancer trap insert in dac.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAbdac1-1 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product mAbdac1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
9 References
Initial Publication
dachshund encodes a nuclear protein required for normal eye and leg development in Drosophila.
Rubin GM
Development (Cambridge, England) 120.12 (1994 Dec): 3473-86.