1D12

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$40.00
SKU: 1D12
View product citations for antibody 1D12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Gurken protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schupbach, T.
Isotype: MIgG1, kappa light chain
Antigen Sequence: a TGF-alpha-like protein
Host Species: mouse
Depositors Institution: Dept. of Molecular Biology, Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The Chemical properties of the antigen are Grk protein : 294 aa, predicted ~33 kDa (but from work with rat polyclonal anti-Grk, appears to run at 47 kDa).
Antigen Molecular Weight: 33 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: GST-gurken protein fusion, containing amino acids 53-185 of Grk (extracellular domain)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation of SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528273 
Additional Information: The immunization in 1998 used 50 micrograms plus 50 micrograms plus 100 micrograms plus 25 micrograms. Using intraperitoneal route the adjuvant corixa MPL & TDM emulsion was utilized. RRID:AB_528273
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D12 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product 1D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References
    All References

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