Antigen: Gurken protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schupbach, T.
Isotype: MIgG1, kappa light chain
Antigen Sequence: a TGF-alpha-like protein
Host Species: mouse
Depositors Institution: Dept. of Molecular Biology, Princeton University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The Chemical properties of the antigen are Grk protein : 294 aa, predicted ~33 kDa (but from work with rat polyclonal anti-Grk, appears to run at 47 kDa).
Antigen Molecular Weight: 33 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: GST-gurken protein fusion, containing amino acids 53-185 of Grk (extracellular domain)
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Designation of SP2/0
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_528273
Additional Information: The immunization in 1998 used 50 micrograms plus 50 micrograms plus 100 micrograms plus 25 micrograms. Using intraperitoneal route the adjuvant corixa MPL & TDM emulsion was utilized. RRID:AB_528273
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D12 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product 1D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).