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SKU: 1D12

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 1D12
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528273 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: GST-gurken protein fusion, containing amino acids 53-185 of Grk (extracellular domain)
Immunogen Sequence:
Myeloma Strain: Designation of SP2/0
Epitope Mapped: No
Antigen Name: Gurken protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/25/2001
Antigen Molecular Weight: 33 kDa
Depositor: Schupbach, T.
Antigen Sequence: a TGF-alpha-like protein
Depositor Institution: Princeton University
Antigen Species: Drosophila
Depositor Notes: The Chemical properties of the antigen are Grk protein : 294 aa, predicted ~33 kDa (but from work with rat polyclonal anti-Grk, appears to run at 47 kDa).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: The immunization in 1998 used 50 micrograms plus 50 micrograms plus 100 micrograms plus 25 micrograms. Using intraperitoneal route the adjuvant corixa MPL & TDM emulsion was utilized. RRID:AB_528273
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D12 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product 1D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References
    All References

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