LAM-B

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$45.00 to $425.00
SKU: LAM-B
View product citations for antibody LAM-B on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: laminin, murine parietal yolk sac
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Damjanov, I.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Kansas University Medical Center
Positive Tested Species Reactivity: Mouse
Depositors Notes: Mouse laminin, yolk sac specific. This antibody reacts only with parietal yolk sac laminin, 6-18 day yolk sac of mouse; does not react with adult basement membrane or rat yolk sac.
Antigen Molecular Weight: Apparent: 200 kDa (beta and gamma) and 400 kDa (alpha)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Mouse yolk sac carcinoma cells derived from NF-1 teratocarcinoma.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3-X63-Ag-8.653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528340 
Additional Information: Immunoblotting: LAM-B doesn't work well on denatured laminin.
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LAM-B was deposited to the DSHB by Damjanov, I. (DSHB Hybridoma Product LAM-B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Distinct antigenic characteristics of murine parietal yolk sac laminin.
    Damjanov I
    Developmental biology 121.2 (1987 Jun): 397-407.

    IF References
    IP References

    Distinct antigenic characteristics of murine parietal yolk sac laminin.
    Damjanov I
    Developmental biology 121.2 (1987 Jun): 397-407.

    ELISA References

    Distinct antigenic characteristics of murine parietal yolk sac laminin.
    Damjanov I
    Developmental biology 121.2 (1987 Jun): 397-407.

    All References

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