LAM-B

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$40.00
SKU: LAM-B
View product citations for antibody LAM-B on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: laminin, murine parietal yolk sac
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Damjanov, I.
Isotype: RIgG2a
Depositors Institution: Kanas University Medical Center
Host Species: rat
Depositors Notes: Mouse laminin, yolk sac specific. This antibody reacts only with parietal yolk sac laminin, 6-18 day yolk sac of mouse; does not react with adult basement membrane or rat yolk sac.
Positive Tested Species Reactivity: Mouse
Clonality: Monoclonal
Antigen Molecular Weight: Apparent: 200 kDa (beta and gamma) and 400 kDa (alpha)
Epitope Mapped: No
Immunogen: Mouse yolk sac carcinoma cells derived from NF-1 teratocarcinoma.
Immunogen Sequence: full length protein
Myeloma Strain: P3-X63-Ag-8.653
Additional Information: Immunoblotting: LAM-B doesn't work well on denatured laminin.
Antibody Registry ID: AB_528340 
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LAM-B was deposited to the DSHB by Damjanov, I. (DSHB Hybridoma Product LAM-B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Distinct antigenic characteristics of murine parietal yolk sac laminin.
    Damjanov I
    Developmental biology 121.2 (1987 Jun): 397-407.

    IF References
    IP References

    Distinct antigenic characteristics of murine parietal yolk sac laminin.
    Damjanov I
    Developmental biology 121.2 (1987 Jun): 397-407.

    ELISA References

    Distinct antigenic characteristics of murine parietal yolk sac laminin.
    Damjanov I
    Developmental biology 121.2 (1987 Jun): 397-407.

    All References

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