anti-Bicaudal-D 1B11
Catalog Fields
Antigen: Bicaudal-D
Hybridoma Cells Available: Yes
Antigen Species: drosophila
Depositor: Steward, R.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Waksman Institute, Rutgers University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Fails to detect BicD in some loss-of-function bicD alleles and alleles missing the C-terminal.
Predicted Species Reactivity:
Gene: BicD
Immunogen: Recombinant protein
Alternate Gene Names: almond; anon-EST; Bic-D; Dmel\CG6605
Alternate Antibody Name: 1B11
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: C-terminus (aa 544-782)
Uniprot ID: P16568
Immunogen Sequence: Full length protein
Entrez Gene ID: 35051
Antibody Registry ID: AB_528102
Additional Information: 4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Bicaudal-D 1B11 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-Bicaudal-D 1B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
Steward R
Cell 67.5 (1991 Nov 29): 917-26.