anti-Bicaudal-D 1B11

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SKU: anti-Bicaudal-D 1B11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: anti-Bicaudal-D 1B11
Available to For-Profits: Yes
Alternate Antibody Name: 1B11
Gene Name: BicD
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528102 
Uniprot ID: P16568 
RRID:  
Entrez Gene ID: 35051 
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Bicaudal-D
Epitope Location or Sequence: C-terminus (aa 544-782)
Alternate Antigen Name:
Deposit Date: 3/20/2003
Antigen Molecular Weight: Predicted: 89kDa; Apparent: 97kDa
Depositor: Steward, R.
Antigen Sequence:
Depositor Institution: Rutgers University, Waksman Institute
Antigen Species: drosophila
Depositor Notes: Fails to detect BicD in some loss-of-function bicD alleles and alleles missing the C-terminal.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: 4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Bicaudal-D 1B11 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-Bicaudal-D 1B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FB References
    Epitope Map References
    All References

    Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
    Steward R
    Cell 67.5 (1991 Nov 29): 917-26.

    Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.
    Suter B
    Development (Cambridge, England) 141.9 (2014 May): 1915-26.

    The mRNA transportome of the BicD/Egl transport machinery.
    Suter B
    RNA biology 14.1 (2017 Jan 2): 73-89.

    The Ste20-like kinase misshapen functions together with Bicaudal-D and dynein in driving nuclear migration in the developing drosophila eye.
    Rao Y
    Mechanisms of development 122.1 (2005 Jan): 97-108.

    Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 134.7 (2007 Apr): 1419-30.

    Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis.
    Ish-Horowicz D
    Nature 414.6864 (2001 Dec 6): 611-6.

    Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.
    Bullock SL
    The Journal of cell biology 202.3 (2013 Aug 5): 479-94.

    Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor.
    Bullock SL
    Genes & development 23.13 (2009 Jul 1): 1546-58.

    Functional domains of the Drosophila bicaudal-D protein.
    Steward R
    Genetics 154.2 (2000 Feb): 713-24.

    Bicaudal-D regulates fragile X mental retardation protein levels, motility, and function during neuronal morphogenesis.
    Bullock SL
    Current biology : CB 20.16 (2010 Aug 24): 1487-92.

    Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate.
    Lehmann R
    Nature cell biology 6.5 (2004 May): 427-35.

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