anti-Bicaudal-D 1B11

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$40.00
SKU: anti-Bicaudal-D 1B11
View product citations for antibody anti-Bicaudal-D 1B11 on CiteAb

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Available: 176

DSHB Data Sheet

Catalog Fields

Antigen: Bicaudal-D
Hybridoma Cells Available: Yes
Antigen Species: drosophila
Depositor: Steward, R.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Waksman Institute, Rutgers University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Fails to detect BicD in some loss-of-function bicD alleles and alleles missing the C-terminal.
Antigen Molecular Weight: Predicted: 89kDa; Apparent: 97kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: BicD
Immunogen: Recombinant protein
Alternate Gene Names: almond; anon-EST; Bic-D; Dmel\CG6605
Alternate Antibody Name: 1B11
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: C-terminus (aa 544-782)
Uniprot ID: P16568 
Immunogen Sequence: Full length protein
Entrez Gene ID: 35051 
Additional Characterization:  
Antibody Registry ID: AB_528102 
Additional Information: 4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Bicaudal-D 1B11 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-Bicaudal-D 1B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FB References
    Epitope Map References
    All References

    Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
    Steward R
    Cell 67.5 (1991 Nov 29): 917-26.

    Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.
    Suter B
    Development (Cambridge, England) 141.9 (2014 May): 1915-26.

    The mRNA transportome of the BicD/Egl transport machinery.
    Suter B
    RNA biology 14.1 (2017 Jan 2): 73-89.

    The Ste20-like kinase misshapen functions together with Bicaudal-D and dynein in driving nuclear migration in the developing drosophila eye.
    Rao Y
    Mechanisms of development 122.1 (2005 Jan): 97-108.

    Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 134.7 (2007 Apr): 1419-30.

    Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis.
    Ish-Horowicz D
    Nature 414.6864 (2001 Dec 6): 611-6.

    Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.
    Bullock SL
    The Journal of cell biology 202.3 (2013 Aug 5): 479-94.

    Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor.
    Bullock SL
    Genes & development 23.13 (2009 Jul 1): 1546-58.

    Functional domains of the Drosophila bicaudal-D protein.
    Steward R
    Genetics 154.2 (2000 Feb): 713-24.

    Bicaudal-D regulates fragile X mental retardation protein levels, motility, and function during neuronal morphogenesis.
    Bullock SL
    Current biology : CB 20.16 (2010 Aug 24): 1487-92.

    Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate.
    Lehmann R
    Nature cell biology 6.5 (2004 May): 427-35.

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