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SKU: osa
View product citations for antibody osa on CiteAb

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Available: 46

DSHB Data Sheet

Catalog Fields

Antigen: Osa,trithorax group protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This monoclonal antibody was originally named 15A8. Osa protein is also known as eyelid protein.
Antigen Molecular Weight: 284 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: OSA
Immunogen: GST fusion protein with OSA ARID domain
Alternate Gene Names: eld
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q8IN94 
Immunogen Sequence: a.a. 965-1049
Entrez Gene ID: 42130 
Additional Characterization:  
Antibody Registry ID: AB_528420 
Additional Information: FBgn0261885. RRID: AB_528420
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
osa was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product osa)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    All References

    eyelid antagonizes wingless signaling during Drosophila development and has homology to the Bright family of DNA-binding proteins.
    Heberlein U
    Genes & development 11.15 (1997 Aug 1): 1949-62.

    Osa associates with the Brahma chromatin remodeling complex and promotes the activation of some target genes.
    Treisman JE
    The EMBO journal 18.24 (1999 Dec 15): 7029-40.

    Chromatin proteins and RNA are associated with DNA during all phases of mitosis.
    Mazo A
    Cell discovery 2. (2016): 16038.

    Stepwise histone modifications are mediated by multiple enzymes that rapidly associate with nascent DNA during replication.
    Mazo A
    Nature communications 4. (2013): 2841.

    The Osa-containing SWI/SNF chromatin-remodeling complex regulates stem cell commitment in the adult Drosophila intestine.
    Hou SX
    Development (Cambridge, England) 140.17 (2013 Sep): 3532-40.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    Earmuff restricts progenitor cell potential by attenuating the competence to respond to self-renewal factors.
    Lee CY
    Development (Cambridge, England) 141.5 (2014 Mar): 1036-46.

    Akirin links twist-regulated transcription with the Brahma chromatin remodeling complex during embryogenesis.
    Baylies MK
    PLoS genetics 8.3 (2012): e1002547.

    Hox proteins mediate developmental and environmental control of autophagy.
    Graba Y
    Developmental cell 28.1 (2014 Jan 13): 56-69.

    SWI/SNF complex prevents lineage reversion and induces temporal patterning in neural stem cells.
    Knoblich JA
    Cell 156.6 (2014 Mar 13): 1259-1273.

    p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes.
    Dyson NJ
    Molecular and cellular biology 24.20 (2004 Oct): 9124-36.

    A comprehensive platform for the analysis of ubiquitin-like protein modifications using in vivo biotinylation.
    Sutherland JD
    Scientific reports 7. (2017 Jan 18): 40756.

    The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions.
    Hirose S
    Development (Cambridge, England) 139.24 (2012 Dec): 4582-90.

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