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SKU: UNC93-5.2.1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: UNC93-5.2.1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: His2Av
Ab Isotype: MIgG2b, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2618077 
Uniprot ID: P08985 
Entrez Gene ID: 43229 
Clonality: Monoclonal
Immunogen: Phosphorylated peptide conjugated to KLH
Immunogen Sequence: QDPQRKGNVILpSQAY (15 residues at C-terminus)
Myeloma Strain: P3X63-AG8.653
Epitope Mapped: Yes
Antigen Name: histone 2A gamma variant, phosphorylated
Epitope Location or Sequence: C-terminus
Alternate Antigen Name:
Deposit Date: 9/9/2013
Antigen Molecular Weight: 15 kDa
Depositor: Hawley, R.S.
Antigen Sequence:
Depositor Institution: Stowers Institute
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: FLYBASE: FBgn0001197
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
UNC93-5.2.1 was deposited to the DSHB by Hawley, R.S. (DSHB Hybridoma Product UNC93-5.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ChIP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ChIP References
    Epitope Map References
    All References

    Polyploid mitosis and depolyploidization promote chromosomal instability and tumor progression in a Notch-induced tumor model.
    Deng WM
    Developmental cell 56.13 (2021 Jul 12): 1976-1988.e4.

    The development of a monoclonal antibody recognizing the Drosophila melanogaster phosphorylated histone H2A variant (γ-H2AV).
    Hawley RS
    G3 (Bethesda, Md.) 3.9 (2013 Sep 4): 1539-43.

    A role for tuned levels of nucleosome remodeler subunit ACF1 during Drosophila oogenesis.
    Becker PB
    Developmental biology 411.2 (2016 Mar 15): 217-230.

    Early development of Drosophila embryos requires Smc5/6 function during oogenesis.
    Kegel A
    Biology open 5.7 (2016 Jul 15): 928-41.

    Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila.
    Igaki T
    Nature communications 5. (2014 Oct 27): 5264.

    Heterochromatic breaks move to the nuclear periphery to continue recombinational repair.
    Chiolo I
    Nature cell biology 17.11 (2015 Nov): 1401-11.

    A Robust Transposon-Endogenizing Response from Germline Stem Cells.
    Zhang ZZ
    Developmental cell 47.5 (2018 Dec 3): 660-671.e3.

    Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    Cooperation between oncogenic Ras and wild-type p53 stimulates STAT non-cell autonomously to promote tumor radioresistance.
    Chabu CY
    Communications biology 4.1 (2021 Mar 19): 374.

    Drosophila p53 isoforms have overlapping and distinct functions in germline genome integrity and oocyte quality control.
    Calvi BR
    eLife 11. (2022 Jan 13): .

    Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.
    MacAlpine DM
    Nucleic acids research 44.15 (2016 Sep 6): 7204-18.

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