Catalog Fields
Antigen: histone 2A gamma variant, phosphorylated
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b, kappa light chain
Depositor: Hawley, R.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Stowers Institute
Antigen Molecular Weight: 15 kDa
Depositors Notes:
Immunogen: Phosphorylated peptide conjugated to KLH
Gene: His2Av
Alternate Antibody Name:
Alternate Gene Names: gamma-H2Av
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63-AG8.653
Epitope Mapped: Yes
Uniprot ID: P08985
Epitope Location or Sequence: C-terminus
Entrez Gene ID: 43229
Immunogen Sequence: QDPQRKGNVILpSQAY (15 residues at C-terminus)
Antibody Registry ID: AB_2618077
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information: FLYBASE: FBgn0001197
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
UNC93-5.2.1 was deposited to the DSHB by Hawley, R.S. (DSHB Hybridoma Product UNC93-5.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
The development of a monoclonal antibody recognizing the Drosophila melanogaster phosphorylated histone H2A variant (γ-H2AV).
Hawley RS
G3 (Bethesda, Md.) 3.9 (2013 Sep 4): 1539-43.